Disinfection and inoculation method of microphyte seeds and pollen

A plant seed and pollen technology, applied in botany equipment and methods, plant genetic improvement, application, etc., can solve the problems of long germination time and low germination rate, and achieve simple and easy operation, speed up inoculation, reduce Effect of Pollution Rate

Inactive Publication Date: 2016-02-17
YANGTZE UNIVERSITY
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AI Technical Summary

Problems solved by technology

In addition, the major defect of live seedlings is that the germination rate is lower than 5%, and it takes a long time to germinate. It takes about 3-4 months to germinate, differentiate and sprout, and it takes 1 and a half years to grow into 5-10cm seedlings.

Method used

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  • Disinfection and inoculation method of microphyte seeds and pollen

Examples

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Effect test

Embodiment 1

[0034] Disinfection and inoculation of Bletilla striata fruit pods.

[0035] Take 10-15 pods of the orchid plant Bletilla striata, rinse them under tap water for 30 minutes, use sterile filter paper to dry the remaining water on the surface of the pods on an ultra-clean workbench, then soak them in 75% alcohol and rinse them for 2- After 3 minutes, put the fruit pods in a sterile petri dish with sterilized absorbent paper, first absorb the water on the fruit pods, and then cut the fruit pods of Bletilla striata on the previous petri dish, remove Fruit pod shell, cut Bletilla striata seeds, set aside. Put the surface-sterilized Bletilla striata seeds into a container on a sterile operating table, add 75% ethanol solution, shake the container gently until the seeds are completely contacted by the disinfectant, and after sterilizing for 30s-1min, suck out the ethanol solution with a syringe , leaving only the seeds. Then put 2% sodium hypochlorite solution into the container, a...

Embodiment 2

[0037] Disinfection and inoculation of rice pollen

[0038] Take 10-15 healthy and undamaged rice anthers or pollen sacs, rinse them under running water for 30 minutes, then put them in a sterile petri dish on a sterile operating table, and use sterilized forceps to remove the pollen inside Squeeze out the grains, add 70% ethanol solution, shake the container gently until the single grains of pollen are completely in contact with the disinfectant, and after 20-30 seconds of disinfection, suck out the ethanol solution with a syringe, leaving only single grains of pollen. Then put 2% sodium hypochlorite solution into the container, and after sterilizing the rice pollen for 5-8 minutes, use a syringe to suck out the sodium hypochlorite solution into the waste liquid tank next to the aseptic operating table, and finally use sterile water to disinfect the pollen according to the above method. Wash the pollen 3 to 5 times. Finally, add sterile water to the sterilized pollen grains pl...

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Abstract

The invention discloses a disinfection and inoculation method of microphyte seeds and pollen. A special disinfection and inoculation technology for small seeds and plant pollen in a tissue culture process is designed by utilizing seeds and plant pollen of microphytes including orchids as materials in combination of a disinfection method of the tissue culture technology and tools such as an injector and the like; according to the technology, the problems that effective disinfection cannot be realized and a disinfectant cannot be completely removed due to too small orchid seeds and most plant pollen are solved, and the contamination rate in the tissue culture can be effectively reduced to be lower than 5%. Besides, with the adoption of the technology, disinfection and inoculation speeds can be greatly increased, meanwhile, due to the fact that a low-concentration sodium hypochlorite solution can effectively destroy seed coats of the microphyte seeds and cell walls of the pollen and cannot damage seed embryos and pollen cells, accordingly, the microphyte seeds of the plants such as the orchids and the like or the pollen cells of the plants can absorb nutrition of a culture medium more easily, and the seeds germinate 3-5 days in advance.

Description

technical field [0001] The invention belongs to the technical field of agricultural inoculation, and in particular relates to a method for disinfection and inoculation of tiny plant seeds and pollen. Background technique [0002] Orchidaceae (Orchidaceae) plants are a large group of angiosperms, and many Orchidaceae plants have high value in ornamental and medicinal aspects. However, due to the destruction of its habitat due to a large number of plundering excavations, the population of wild orchid resources has declined sharply. The seeds of orchids are extremely small and have no endosperm, so it is difficult to germinate into seedlings in a natural state. However, many orchid plants are also high-end medicinal plants, such as: bletilla striata, dendrobium, gastrodia elata, etc., which have irreplaceable medicinal effects in Chinese medicinal materials. [0003] Bletilla striata (Bletillasfriata (Thunb.) Reiehb.f.) is a terrestrial orchid plant, and its main medicinal pa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02
Inventor 周燚余磊磊江建伟孙正祥邓建新龚天芝方守国
Owner YANGTZE UNIVERSITY
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