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Highly effective cultivating method for Chinese caterpillar fungus

A cultivation method and a technology for Cordyceps militaris are applied in the field of fungal biological cultivation, can solve problems such as the difficulty of industrialized production, and achieve the effects of shortened cultivation time, fast growth rate and short fermentation time

Inactive Publication Date: 2010-09-08
JIANGSU UNIV OF SCI & TECH
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Problems solved by technology

But these silkworm caterpillar fungus all are to utilize solid strain to cultivate, promptly at first inoculate the mother seed of solid strain on the solid culture medium, through step by step amplification production solid strain cultivated species, then inoculate on the culture medium such as silkworm chrysalis or rice etc. and produce. into, industrial production is more difficult

Method used

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Embodiment Construction

[0015] Concrete preparation steps of the present invention are as follows:

[0016] 1. Preparation of liquid medium. According to the ratio of N (such as yeast powder) : C (such as glucose) of 1:2, 0.05% MgSO 4 , 0.1% KH 2 PO 4 , 0.2% peptone and 3-4% viscosifier, after dissolving, adjust the pH to 6.5-7.0 to make a liquid medium, and then make a sterile liquid medium through procedures such as autoclaving;

[0017] 2. Cordyceps militaris strain inoculation and fermentation culture. Inoculate highly pathogenic Cordyceps militaris strains (such as Cordyceps militaris Link) into a sterile liquid medium in a sterile environment, and aerate and ferment in a fermenter for 3-5 days until a large amount of mycelium is formed;

[0018] 3. Cultivation of Cordyceps militaris. Inoculate the Cordyceps militaris mycelia formed after fermentation into sterilized silkworm chrysalis or on other mediums such as rice. 14-18 hours of light) and ventilation and other Cordyceps growth enviro...

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Abstract

The invention discloses a highly effective culture method of Chinese caterpillar fungus. The method is that with the liquid fermentation of Chinese caterpillar fungus strain, a large number of Chinese caterpillar fungus mycelium are cultivated after a few days; the cultivated species of Chinese caterpillar fungus can be produced in a large scale; then the Chinese caterpillar fungus mycelium produced after fermentation are inoculated on silkworm chrysalis or rice medium which is treated by surface sterilization; the further cultivation of the Chinese caterpillar fungus mycelium are processed at a certain appropriate temperature and humidity with necessary ventilation and light management; a large amount of artificial cordyceps militaris which has same form and similar function with the wild Chinese caterpillar fungus is obtained after a few days. The highly effective culture method of Chinese caterpillar fungus of the invention has not only rapidness and effectiveness but also low culture cost. The invention has important significance and popularization and application value for the development of precious Chinese medicine resource, the extension of novel application of .silkworm industry resource and the improvement of additional value.

Description

technical field [0001] The invention relates to the field of fungal biological cultivation, in particular to an efficient cultivation method of Cordyceps militaris. Background technique [0002] Cordyceps militaris has a variety of health and physiological functions such as improving immunity, anti-tumor, and anti-oxidation. The cultivation method of Cordyceps militaris has many reports, such as the artificial cultivation method of Cordyceps militaris (ZL87106987.3); the artificial cultivation method of Cordyceps militaris etc. (ZL97113190.2); the industrial production method of Cordyceps militaris (ZL00130381.3) etc. But these silkworm caterpillar fungus all are to utilize solid strain to cultivate, promptly at first inoculate the mother seed of solid strain on the solid culture medium, through step by step amplification production solid strain cultivated species, then inoculate on the culture medium such as silkworm chrysalis or rice etc. and produce. It is difficult to i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14
Inventor 桂仲争朱雅红侯成香
Owner JIANGSU UNIV OF SCI & TECH
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