Inoculation method of tissue culture seedlings of potatoes

Abstract

The invention discloses an inoculation method of tissue culture seedlings of potatoes. The inoculation method is characterized in that impact factors of the growth of the detoxified tissue culture seedlings of the potatoes are strictly controlled, and the tissue culture seedlings are subjected to integral cutting, dispersed tissue culture and cutting again after inoculation appliances, a culture room and an inoculation room are strictly disinfected and sterilized, so that the purposes of high speed, short cycle, low pollution, simple operation and low cost of the detoxified tissue culture and breeding of the potatoes are achieved.

Description

technical field

[0001] The invention belongs to the technical field of plant cultivation, in particular to a method for inoculating potato tissue culture seedlings. Background technique

[0002] With the continuous development of agricultural technology, potato detoxification technology has been widely used. The so-called virus-free means: cut out the growth cones of potato shoots at 0.2-0.3 mm, carry out tissue culture, cultivate virus-free test-tube seedlings, and then produce virus-free tubers. It has become the best method for preventing and controlling potato viruses and increasing potato yields. method. Combining shoot tip virus-free, tissue culture, and virus-free seed potato breeding to form a complete virus-free rapid propagation system can truly achieve the purpose of fast potato breeding and good quality.

[0003] However, in the existing virus-free tissue culture technology, the investment in tissue culture is large and the economic benefit is low, which has se...

Examples

Embodiment 1

[0022] Select and cultivate 10 strains of strong tissue-cultured virus-free seedlings with a seedling age of 15 days, and then adopt the technical scheme of the present invention to inoculate.

[0023] 1. Disinfection and sterilization

[0024] (1) Disinfection and sterilization of the medium: the medium used for the tissue culture of the virus-free seedlings of the present invention is a liquid medium, and the prepared nutrient base is sterilized under high temperature and high pressure for 20 minutes, wherein the temperature is 121 ° C, the air pressure 1.11-1.14kpa;

[0025] (2) Disinfection and sterilization of inoculation equipment: irradiate the inoculation room, cultivation room, preparation room, etc. with ultraviolet light, mop and wash the floor with 0.1% benzalkonium bromide, and fumigate with formazan and potassium permanganate from time to time; Soak the scissors, tweezers, and petri dishes with 75% alcohol before inoculation; the scissors and tweezers after soak...

Embodiment 2

[0032] Select and cultivate 10 strains of strong tissue-cultured virus-free seedlings with a seedling age of 15 days, and then adopt the technical scheme of the present invention to inoculate.

[0033] 1. Disinfection and sterilization

[0034] (1) Disinfection and sterilization of the medium: the medium used for the tissue culture of the virus-free seedlings of the present invention is a liquid medium, and the prepared nutrient base is sterilized under high temperature and high pressure for 20 minutes, wherein the temperature is 121 ° C, the air pressure 1.11-1.14kpa;

[0035] (2) Disinfection and sterilization of inoculation equipment: irradiate the inoculation room, cultivation room, preparation room, etc. with ultraviolet light, mop and wash the floor with 0.1% benzalkonium bromide, and fumigate with formazan and potassium permanganate from time to time; Soak the scissors, tweezers, and petri dishes with 75% alcohol before inoculation; the scissors and tweezers after soak...

technical field

[0001] The invention belongs to the technical field of plant cultivation, in particular to a method for inoculating potato tissue culture seedlings. Background technique

[0002] With the continuous development of agricultural technology, potato detoxification technology has been widely used. The so-called virus-free means: cut out the growth cones of potato shoots at 0.2-0.3 mm, carry out tissue culture, cultivate virus-free test-tube seedlings, and then produce virus-free tubers. It has become the best method for preventing and controlling potato viruses and increasing potato yields. method. Combining shoot tip virus-free, tissue culture, and virus-free seed potato breeding to form a complete virus-free rapid propagation system can truly achieve the purpose of fast potato breeding and good quality.

[0003] However, in the existing virus-free tissue culture technology, the investment in tissue culture is large and the economic benefit is low, which has se...

Examples

Embodiment 1

[0022] Select and cultivate 10 strains of strong tissue-cultured virus-free seedlings with a seedling age of 15 days, and then adopt the technical scheme of the present invention to inoculate.

[0023] 1. Disinfection and sterilization

[0024] (1) Disinfection and sterilization of the medium: the medium used for the tissue culture of the virus-free seedlings of the present invention is a liquid medium, and the prepared nutrient base is sterilized under high temperature and high pressure for 20 minutes, wherein the temperature is 121 ° C, the air pressure 1.11-1.14kpa;

[0025] (2) Disinfection and sterilization of inoculation equipment: irradiate the inoculation room, cultivation room, preparation room, etc. with ultraviolet light, mop and wash the floor with 0.1% benzalkonium bromide, and fumigate with formazan and potassium permanganate from time to time; Soak the scissors, tweezers, and petri dishes with 75% alcohol before inoculation; the scissors and tweezers after soak...

Embodiment 2

[0032] Select and cultivate 10 strains of strong tissue-cultured virus-free seedlings with a seedling age of 15 days, and then adopt the technical scheme of the present invention to inoculate.

[0033] 1. Disinfection and sterilization

[0034] (1) Disinfection and sterilization of the medium: the medium used for the tissue culture of the virus-free seedlings of the present invention is a liquid medium, and the prepared nutrient base is sterilized under high temperature and high pressure for 20 minutes, wherein the temperature is 121 ° C, the air pressure 1.11-1.14kpa;

[0035] (2) Disinfection and sterilization of inoculation equipment: irradiate the inoculation room, cultivation room, preparation room, etc. with ultraviolet light, mop and wash the floor with 0.1% benzalkonium bromide, and fumigate with formazan and potassium permanganate from time to time; Soak the scissors, tweezers, and petri dishes with 75% alcohol before inoculation; the scissors and tweezers after soak...