Chinese saprophytic boletus liquid strain culture medium and culture method
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A technology of liquid strains and culture methods, applied in the field of microorganisms, can solve the problems of long germination time of strains, long preparation cycle, slow mycelium growth, etc.
Pending Publication Date: 2021-09-07
YUNNAN INST OF TROPICAL CROPS
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[0004] The Chinese saprophytic boletus cultivars are inoculated with solid strains, the germination time of the strains is long, and the mycelium grows slowly, resulting in a long preparation period for the Chinese saprophy
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Embodiment 1
[0027] (1) a kind of Chinese saprophytic boletus liquid strain culture medium, the culture medium formula is:
[0028] Potato: 200g
[0029] Bran: 15g
[0030] Glucose: 15g
[0031] Yeast powder: 2g
[0032] CaCO 3 : 2g
[0033] K H 2 PO 4 : 2g
[0034] MgSO 4 : 1g
[0035] Vitamin B 1 :2 tablets
[0036] Water: 1000mL.
[0037] (2) a kind of Chinese saprophytic boletus liquid strain culture method:
[0038] a. Culture medium preparation: weigh potatoes, bran, glucose, yeast powder, CaCO 3 、 KH 2 PO 4 , MgSO 4 , Vitamin B 1 , wash, peel and slice 200g of potatoes, wrap 15g of bran with 4 layers of gauze, put the potatoes and bran in 1000mL tap water, boil for 25min; Add glucose 15g, yeast powder 2g, CaCO 3 2g, KH 2 PO 4 2g, MgSO 4 1g, vitamin B 1 2 tablets (20mg), dilute to 1000mL with tap water to obtain the Chinese saprophytic boletus liquid culture medium;
[0039] b. Subpackage: Subpackage the prepared medium into 500mL Erlenmeyer flasks, each w...
Embodiment 2
[0044] (1) a kind of Chinese saprophytic boletus liquid strain culture medium, the culture medium formula is:
[0045] Potato: 150g
[0046] Bran: 20g
[0047] Glucose: 15g
[0048] Yeast powder: 3g
[0049] CaCO 3 : 2g
[0050] K H 2 PO 4 : 3g
[0051] MgSO 4 : 2g
[0052] Vitamin B 1 :1
[0053] Water: 1000mL.
[0054] (2) a kind of Chinese saprophytic boletus liquid strain culture method:
[0055] a. Culture medium preparation: weigh potatoes, bran, glucose, yeast powder, CaCO 3 、 KH 2 PO 4 , MgSO 4 , Vitamin B 1 , wash, peel and slice 150g potatoes, wrap 20g bran with 4 layers of gauze, put the potatoes and bran in 1000mL tap water, boil for 20-25min; filter with 4 layers of gauze to obtain potato and bran extract, Then add glucose 15g, yeast powder 3g, CaCO 3 2g, KH 2 PO 4 3g, MgSO 4 2g, vitamin B 1 1 tablet (10mg), dilute to 1000mL with tap water to obtain a liquid culture medium for Boletus saprophyticus;
[0056] b. Subpackage: Subpackage the ...
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Abstract
The invention belongs to the field of microorganisms, and discloses a Chinese saprophytic boletus liquid strain culture medium and a culture method. The culture medium is prepared from 150 to 200 g of potatoes, 10 to 25 g of bran, 10 to 15 g of glucose, 2 to 3 g of yeast powder, 1 to 2 g of CaCO3, 2 to 3 g of KH2PO4, 1 to 2 g of MgSO4, 1 to 2 tablets of vitamin B1 and 1000 mL of water. The culture medium provided by the invention is suitable for rapid growth of the Chinese saprophytic boletus, the rotating speed of a shaking table is controlled in different periods according to the growth characteristics of mycelia in the culture process, the recovery, germination and growth of the mycelia are promoted, the method is simple to operate and low in cost, mycelium pellets are fine and uniform, after mushroom sticks are inoculated, the mycelium pellets germinate in a multi-point mode, and therefore, the contact area between the mycelia and the culture material is effectively increased; compared with a solid strain, the mycelia are high in growth speed, low in pollution rate and consistent in strain age, and the strain culture period is effectively shortened.
Description
technical field [0001] The invention relates to the field of microorganisms, in particular to a liquid culture medium and a culture method of boletus saprophyticus sinensis. Background technique [0002] Yunnan Institute of Tropical Crops Science and Hainan Medical College conducted molecular identification and biological characteristics research on wild Boletus sinensis collected from Xishuangbanna Prefecture, Yunnan Province and Haikou City, Hainan Province. In April 2021, Buchwaldoboletus xylophilus Named "Boletus saprophyticus", the Boletus saprophyticus strain BU001 was preserved in the General Microbiology Center of China Committee for Microbial Culture Collection on May 17, 2021, and the preservation number is: CGMCC No.: 21959. Address of Preservation Unit: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. [0003] Chinese saprophytic boletus (Buchwaldoboletus xylophilus) belongs to Fungi Kingdo...
Claims
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