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A boletus and saprophytic technology, applied in the field of microorganisms, can solve the problems of different resistance to impurities, fruiting rate, mushrooming time, unstable yield and quality, and restrictions on the expansion of Chinese saprophytic boletus cultivation.
Active Publication Date: 2021-09-21
YUNNAN INST OF TROPICAL CROPS +1
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[0003] At present, the artificial cultivation of saprophytic boletus in mushroom house has been successful, but different strains have different mycelial properties and resistance to impurities, resulting in unstable fruiting rate, fruiting time, yield and quality, which restricts Chinese saprophytic boletus from the source. Bacteria expanded cultivation
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Embodiment 1
[0017] Embodiment 1 Separation and Identification of Boletus saprophyticus strain
[0018] It was isolated from wild boletus tissue in Jinghong City, Xishuangbanna Prefecture, Yunnan Province, and its morphology, physiological and biochemical properties, and ITS sequencing were identified. The strain was named: Boletus sinensis BU001, belonging to the order Boletes, Boletus, Saprophytic Boletus.
[0019] Boletus saprophyticus strain BU001 was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 17, 2021. The preservation number is: CGMCC No.21959, and the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101.
[0020] 1. Production of mother seed medium: prepare PDA enriched medium.
[0021] 2. Separation of strains: Collect the fruiting bodies of wild Boletus sinensis, and clean up the dead branches, soil, sand ...
Embodiment 2
[0030] Embodiment 2 Chinese saprophytic boletus BU001 bacterial strain cultivation
[0031] 1. Production of the original species of Boletus saprophyticus
[0032] (1) Liquid strain: transfer the parent species of Boletus sinensis BU001 to a liquid medium, place it at 140 r / min, and culture it on a shaker at 27°C in dark light for 6-8 days to obtain a liquid strain.
[0033] (2) Solid stock: insert the stock Boletus sinensis BU001 into the solid seed medium, culture at 27°C in the dark for 45-55 days, and mycelia are covered with fungus bags to obtain the solid stock.
[0034] 2. Production of Chinese Saprophytic Boletus cultivars
[0035] The above-mentioned liquid strains or solid original strains are inserted into the culture medium of Boletus saprotrophus sinensis, cultivated in dark light at 26-28°C for 50-65 days, and the hyphae are covered with fungus bags to obtain cultivars.
[0036] 3. Cultivation and fruiting
[0037] Cover the cultivated species with mycelium fu...
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Abstract
The invention discloses an excellent Chinese saprophytic bolete strain which is stable in cultivation character and high in yield. The Chinese saprophytic bolete strain BU001 is preserved in the China General Microbiological Culture Collection Center on May 17, 2021, and the preservation number of the Chinese saprophytic bolete strain BU001 is CGMCC No. 21959. According to the Chinese saprophytic bolete strain BU001 provided by the invention, a fungal colony is in a round or irregular shape, and hyphae are thick, strong and dense and are yellowish white; when the strain BU001 is used for preparing a liquid strain, hypha pellets are light brown and uniform in size, and the liquid strain can be prepared by fermenting and culturing for 6-8 days; when the strain is used for preparing and cultivating strains, the strain age is consistent, hyphae are golden and dense, the hybrid resistance is high, the spawn running speed is high, fruiting is neat, the yield is high, the cultivation period is short, and large-scale cultivation and production of the Chinese saprophytic boletus are facilitated. Therefore, the Chinese saprophytic boletus strain BU001 has important application value in germplasm resource protection, development, utilization and scientific research of the Chinese saprophytic boletus.
Description
technical field [0001] The invention relates to a strain of Boletus sinensis saprophyticus, in particular to a strain of Boletus sinensis saprophyticus which has stable cultivation properties and high yield and is suitable for artificial cultivation, and belongs to the technical field of microorganisms. Background technique [0002] Chinese saprophytic boletus (Buchwaldoboletus xylophilus) is named for its ability to live a saprophytic life, belongs to the order Boletus, Boletaceae, and the genus Saprophytic Boletus. It is a rare boletus in tropical and subtropical regions; Distributed in Sri Lanka, the Philippines, Malaysia, and domestically distributed in warm regions such as Yunnan, Hainan, and Hong Kong. The fruiting body of Boletus sinensis is medium in size, the cap is yellowish brown or brown, and the edge of the cap is involuted when young; the stipe is reddish brown or brown, and dense yellow mycelium can be seen at the base of the stipe; the flesh is yellow or ligh...
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