A Strain of Boletus sinensis
A boletus and saprophytic technology, applied in the field of microorganisms, can solve the problems that restrict the expansion of Chinese saprophytic boletus cultivation, fruiting rate, fruiting time, unstable yield and quality, and different degrees of resistance to impurities.
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Embodiment 1
[0017] Embodiment 1 Separation and Identification of Boletus saprophyticus strain
[0018] It was isolated from wild boletus tissue in Jinghong City, Xishuangbanna Prefecture, Yunnan Province, and its morphology, physiological and biochemical properties, and ITS sequencing were identified. The strain was named: Boletus sinensis BU001, belonging to the order Boletes, Boletus, Saprophytic Boletus.
[0019] Boletus saprophyticus strain BU001 was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 17, 2021. The preservation number is: CGMCC No.21959, and the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101.
[0020] 1. Production of mother seed medium: prepare PDA enriched medium.
[0021] 2. Separation of strains: Collect the fruiting bodies of wild Boletus sinensis, and clean up the dead branches, soil, sand ...
Embodiment 2
[0030] Embodiment 2 Chinese saprophytic boletus BU001 bacterial strain cultivation
[0031] 1. Production of the original species of Boletus saprophyticus
[0032] (1) Liquid strain: transfer the parent species of Boletus sinensis BU001 to a liquid medium, place it at 140 r / min, and culture it on a shaker at 27°C in dark light for 6-8 days to obtain a liquid strain.
[0033] (2) Solid stock: insert the stock Boletus sinensis BU001 into the solid seed medium, culture at 27°C in the dark for 45-55 days, and mycelia are covered with fungus bags to obtain the solid stock.
[0034] 2. Production of Chinese Saprophytic Boletus cultivars
[0035] The above-mentioned liquid strains or solid stock species are inserted into the culture medium of Boletus saprotrophus sinensis, cultivated in dark light at 26-28°C for 50-65 days, and the hyphae are covered with fungus bags to obtain cultivars.
[0036] 3. Cultivation and fruiting
[0037] Cover the cultivated species after the mycelium ...
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