A Strain of Boletus sinensis

A boletus and saprophytic technology, applied in the field of microorganisms, can solve the problems that restrict the expansion of Chinese saprophytic boletus cultivation, fruiting rate, fruiting time, unstable yield and quality, and different degrees of resistance to impurities.

Active Publication Date: 2021-11-23
YUNNAN INST OF TROPICAL CROPS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the artificial cultivation of saprophytic boletus in mushroom house has been successful, but different strains have different mycelial properties and resistance to impurities, resulting in unstable fruiting rate, fruiting time, yield and quality, which restricts Chinese saprophytic boletus from the source. Bacteria expanded cultivation

Method used

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  • A Strain of Boletus sinensis
  • A Strain of Boletus sinensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 Separation and Identification of Boletus saprophyticus strain

[0018] It was isolated from wild boletus tissue in Jinghong City, Xishuangbanna Prefecture, Yunnan Province, and its morphology, physiological and biochemical properties, and ITS sequencing were identified. The strain was named: Boletus sinensis BU001, belonging to the order Boletes, Boletus, Saprophytic Boletus.

[0019] Boletus saprophyticus strain BU001 was preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee on May 17, 2021. The preservation number is: CGMCC No.21959, and the preservation address is No. 1, Beichen West Road, Chaoyang District, Beijing No. 3 Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101.

[0020] 1. Production of mother seed medium: prepare PDA enriched medium.

[0021] 2. Separation of strains: Collect the fruiting bodies of wild Boletus sinensis, and clean up the dead branches, soil, sand ...

Embodiment 2

[0030] Embodiment 2 Chinese saprophytic boletus BU001 bacterial strain cultivation

[0031] 1. Production of the original species of Boletus saprophyticus

[0032] (1) Liquid strain: transfer the parent species of Boletus sinensis BU001 to a liquid medium, place it at 140 r / min, and culture it on a shaker at 27°C in dark light for 6-8 days to obtain a liquid strain.

[0033] (2) Solid stock: insert the stock Boletus sinensis BU001 into the solid seed medium, culture at 27°C in the dark for 45-55 days, and mycelia are covered with fungus bags to obtain the solid stock.

[0034] 2. Production of Chinese Saprophytic Boletus cultivars

[0035] The above-mentioned liquid strains or solid stock species are inserted into the culture medium of Boletus saprotrophus sinensis, cultivated in dark light at 26-28°C for 50-65 days, and the hyphae are covered with fungus bags to obtain cultivars.

[0036] 3. Cultivation and fruiting

[0037] Cover the cultivated species after the mycelium ...

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Abstract

The invention discloses an excellent strain of Boletus sinensis with stable cultivation properties and high yield. The Boletus saprophyticus strain BU001 was deposited in the General Microorganism Center of China Committee for the Collection of Microorganisms on May 17, 2021, and the preservation number is: CGMCC No.21959. The boletus sinensis saprophyticus BU001 bacterial strain provided by the invention has round or irregular colonies, strong and dense hyphae, and is yellowish-white. The BU001 bacterial strain provided by the invention is used to prepare the liquid strain, the mycelial balls are light brown and uniform in size, and can be produced after 6-8 days of fermentation and cultivation. It is used to make cultivated strains, with consistent bacterial age, golden mycelium, dense, strong resistance to miscellaneous, fast germination, neat fruiting, high yield, short cultivation period, and is conducive to the large-scale cultivation and production of Chinese saprophytic boletus. Therefore, the BU001 strain of Boletus sinensis has important application value in the protection, development, utilization and scientific research of Boletus sinensis germplasm resources.

Description

technical field [0001] The invention relates to a strain of Boletus sinensis saprophyticus, in particular to a strain of Boletus sinensis saprophyticus which has stable cultivation properties and high yield and is suitable for artificial cultivation, and belongs to the technical field of microorganisms. Background technique [0002] Chinese saprophytic boletus (Buchwaldoboletus xylophilus) is named for its ability to live a saprophytic life, belongs to the order Boletus, Boletaceae, and the genus Saprophytic Boletus. It is a rare boletus in tropical and subtropical regions; Distributed in Sri Lanka, the Philippines, Malaysia, and domestically distributed in warm regions such as Yunnan, Hainan, and Hong Kong. The fruiting body of Boletus sinensis is medium in size, the cap is yellowish brown or brown, and the edge of the cap is involuted when young; the stipe is reddish brown or brown, and dense yellow mycelium can be seen at the base of the stipe; the flesh is yellow or ligh...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01G18/40A01G18/00
CPCA01G18/40A01G18/00
Inventor 何明霞杨天伟张春霞许欣景曾念开刘静高锋方艺伟王文兵
Owner YUNNAN INST OF TROPICAL CROPS
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