64 results about "Boletus sp." patented technology

The invention discloses a mother strain culture medium for boletus xylophilus as well as a preparation method and application of the mother strain culture medium, and belongs to the technical field of microbial culture. The preservation number of the strain JSJ-Bx1 of the boletus sinensis mother strain is CGMCC (China General Microbiological Culture Collection Center) No.42007, and the strain JSJ-Bx1 is preserved in the China General Microbiological Culture Collection Center; the culture medium is prepared from 10-30 g of a carbon source, 2-6 g of a nitrogen source, 1-3 g of inorganic salt, 0.1-0.3 g of a growth factor suitable for the culture medium, 20 g of agar and 1 L of water, and the pH of the culture medium is 4-6. By the adoption of the culture medium, hyphae of the boletus sinensis mother strain grow fast, grow vigorously and are dense, the edges of bacterial colonies are neat, hypha degeneration phenomena such as pigment and water spitting are avoided, and the mother strain culture growth cycle is greatly shortened.

The invention relates to a mycorrhizal cultivation method for improving the stress resistance of pinus khasys seedlings, and belongs to the technical field of agricultural planting, the cultivation method comprises the following steps: (1) compounding fibrous belly fungi, bolete and russule virens into a microbial inoculum, and culturing to prepare microcapsules; (2) taking turf, vermiculite, humus soil and perlite as substrates, and adding a microcystis agent and a water-retaining agent; (3) the seeds are disinfected and sown, and seedlings are domesticated through light intensity gradient, water stress and temperature fluctuation; 4, during transplanting, abscisic acid is used for treating root systems, organic fertilizer is pre-filled, and mycorrhizal activation promoting liquid is perfused, through the synergistic effect of composite mycorrhizal fungi and stress domestication, the stress resistance, the survival rate and the afforestation adaptability of pinus khasys seedlings are remarkably improved.

This invention discloses a Chinese saprophytic bolete ( Buchwaldoboletus xylophilus This invention relates to the mother culture medium, its preparation method, and its application, belonging to the field of microbial culture technology. The strain JSJ-Bx1 of the *Boletus spp.* mother culture, with accession number CGMCC No. 42007, is deposited at the China General Microbiological Culture Collection Center. The culture medium composition is: 10–30 g carbon source, 2–6 g nitrogen source, 1–3 g inorganic salts, 0.1–0.3 g suitable growth factors, 20 g agar, and 1 L water, with a pH of 4–6. Using the culture medium of this invention, the mycelium of the *Boletus spp.* mother culture exhibits rapid, vigorous, and dense growth, with neat colony edges, and does not produce pigments or exhibit mycelial degeneration such as exudation, significantly shortening the mother culture growth cycle.

The invention discloses allium fistulosum and bolete polysaccharide as well as a preparation method and application thereof. The allium fistulosum and bolete polysaccharide is a glycopolymer composed of arabinose, xylose, mannose, glucose and galactose. The allium fistulosum bolete polysaccharide disclosed by the invention has strong antioxidant capacity, has a stimulation and activation effect on macrophage immune response, has an inhibition effect on alpha-glucosidase, has the effects of resisting oxidation, enhancing immunity and reducing blood sugar, and can be applied to development of health-care foods and functional foods.

The present invention relates to the field of microbial technology, and discloses Rhizopus microsporus HR3-26 and its application in the extraction of boletus polysaccharide. The present invention provides a new microbial strain, Rhizopus microsporus HR3-26, and allows Rhizopus microsporus HR3-26 to grow moderately in boletus powder added with sucrose to produce various hydrolases. After that, the fermented boletus powder is heated with water, and substances such as cellulose in the cell wall are hydrolyzed, which helps the bound boletus polysaccharide to dissolve during ultrasonic water extraction, thereby significantly improving the extraction yield of polysaccharide. The present invention applies Rhizopus microsporus HR3-26 to the extraction of boletus polysaccharide. Compared with the conventional method of water extraction and alcohol precipitation, the extraction yield of polysaccharide can be increased by 41.3%.

The invention provides a bolete concentrated powder composition lipid carrier with an anti-aging effect as well as a preparation method and application of the bolete concentrated powder composition lipid carrier. The lipid carrier is prepared from the following substances in percentage by mass: 10 to 40 percent of bolete extract, 0.2 to 0.8 percent of antioxidant, 45 to 66 percent of solid lipid, 10 to 16 percent of liquid lipid and 4.2 to 7.8 percent of surfactant. According to the bolete concentrated powder composition lipid carrier provided by the invention, liquid lipid with anti-oxidation and light protection effects is mixed into solid lipid to construct a nano carrier matrix with a light protection function. Plant active ingredients with a light protection effect and an anti-oxidation effect and ergothioneine in the bolete extract are jointly loaded in a nano-structure lipid carrier, so that synergistic interaction of multiple effects is realized. The synergistic delivery system of the nanostructured lipid carrier can inhibit the photodegradation reaction of the ergothioneine and the generation of peculiar smell, improve the photostability of the ergothioneine, enhance the oxidation resistance of the ergothioneine in an acid environment, and improve the anti-aging effect.

The invention discloses boletus brownii xylan palmitate as well as a preparation method and application thereof. The boletus brownii xylan palmitate is xylan palmitate which is formed by alpha-(1-> 4)-xylose as a main chain, alpha-(1->)-glucose as a branch chain and palmitic acid molecules through ester bonds. The invention further discloses a preparation method of the boletus brownii xylan palmitate and application of the boletus brownii xylan palmitate in the aspect of immunoregulation. The boletus brownii xylan palmitate can be used as a dietary supplement or a food additive for immunodeficiency patients and tumor patients.

The present application relates to a kind of compositions with anti-inflammatory and anti-allergic effects and its application, and the preparation raw materials of the composition include boletus, phyllanthus and rose apple.The present application uses steam explosion coupling heating reflux preparation to prepare the composition with boletus, phyllanthus, rose apple as preparation raw material, the method not only significantly improves extraction efficiency and biological activity, compared with traditional extraction technology, also avoids the use of organic reagent, more environmentally friendly and safe.

The invention relates to the field of biological fermentation, in particular to a method for efficiently preparing anti-aging ergothioneine through fermentation of compound bacteria combined with bolete and application thereof.The method comprises the steps that halophilic bacillus SC-1, halophilic archaea IR-2 and thermophilic casein dissolving bacteria PC-3 are mixed according to the volume ratio of 1: 1: 1, bolete and pleurotus citrinopileatus are used as raw materials, and a mixture is prepared through fermentation; fermenting for 120 hours in an optimized basic culture medium under the conditions that the temperature is 30 + / -0.5 DEG C, the speed is 150rpm + / -10rpm and the pH value is 5.8 + / -0.2; through a step-by-step process of purifying EGT by using D101 resin and purifying RNET-1 by using DEAE-52 resin, a double-active-component extract is synchronously obtained. Through the synergistic effect of three strains with complementary functions, an efficient system of raw material degradation, carbon source supply, double precursor synthesis and double active component accumulation is constructed, synchronous high yield of ergothioneine and novel antioxidant protein RNET-1 is realized for the first time, and the blank of single active component in the prior art is filled.

The present application relates to the technical field of fungus cultivation, and particularly relates to a high-nutrition-conversion-rate boletus edulis planting method. The method comprises the following steps: fermenting sugarcane residue to obtain fermented sugarcane residue; mixing the crushed fermented sugarcane residue and eucalyptus wood chips with soybean powder, oat powder and lime powder to obtain a cultivation substrate with a water content of 50-55% by adding water; bagging, sterilizing and cooling the cultivation substrate to obtain a treated cultivation substrate; inoculating boletus edulis liquid strain into the treated cultivation substrate, and culturing in the dark to obtain boletus edulis mycelium; covering the surface of the boletus edulis mycelium with paddy field soil, controlling temperature and humidity, and culturing under light, then adjusting temperature and humidity, and continuing to culture for 5-7 days to obtain high-nutrition-conversion-rate boletus edulis. The planting method effectively improves the nutritional components in boletus edulis, reduces the production cost of boletus edulis, reduces the strain culture time, has strong anti-miscibility, and has high nutrition conversion rate.

The utility model is suitable for the technical field of bolete storage, and provides a novel bolete fresh-keeping box which comprises a box body. The plurality of supporting plates are fixed on the inner wall of the bottom of the box body; the metal box is placed in the box body, the metal box is used for storing bolete, and the bottom of the metal box is in contact with the tops of the multiple supporting plates; the ice storage bin is arranged between the box body and the metal box, and the ice storage bin is filled with ice blocks; and the cover plate is placed at the top of the box body. The novel bolete fresh-keeping box provided by the scheme is convenient to disassemble and clean, meanwhile, the bolete can be isolated and stored, and the situation that the bolete is stacked and crushed to affect the quality of the bolete is prevented.

The invention belongs to the technical field of food processing, and particularly provides a composition with the effects of resisting oxidation and whitening as well as a preparation method and application of the composition with the effects of resisting oxidation and whitening. The health-care food at least comprises the following raw material components in parts by weight: 20-40 parts of a glutathione-enriched yeast extract, 0.5-5 parts of a burdock root extract, 0.5-5 parts of a cordyceps militaris extract, 0.2-2 parts of white tricholoma matsutake freeze-dried powder, 0.2-2 parts of bolete concentrated powder and 20-40 parts of sweet-scented osmanthus powder. The composition provided by the technical scheme is compounded according to a certain proportion, so that the composition has good antioxidant and whitening effects.

The invention discloses a beverage with an immunity enhancing effect and a preparation method thereof. The beverage with the immunity enhancing effect is prepared from the following raw materials in parts by weight: 5-10 parts of liquorice, 5-10 parts of schizophyllumcommuneh, 5-10 parts of endive, 5-10 parts of semen torreya, 3-5 parts of astragalus membranaceus, 5-8 parts of scutellaria baicalensis leaves, 2-3 parts of L-cysteine, 5-8 parts of coffee, 2-3 parts of lemon and 1-3 parts of wild bolete. According to the drink with the immunity enhancing effect, the drink has the effects of tonifying spleen and replenishing qi, nourishing the body, tonifying qi and lifting yang, enhancing the immunity, promoting salivation and soothing the nerves, enhancing the physique, relieving fatigue and the like by limiting all the raw materials and the dosage, and under the combined action of all the raw materials, the symptoms of easy tiredness, fatigue and easy cold caused by low immunity can be relieved; therefore, the beverage with the effect of enhancing the immunity can improve the physique of the human body and enhance the immunity, and the effect is remarkable.

The present invention discloses a Boletus aereus strain AC2266 and its application. This strain is obtained by self-crossing of YL1701-2, and was deposited in the China General Microbiological Culture Collection Center (CGMCC) on March 3, 2021. The deposit number is CGMCC NO.21923, and the taxonomic name is: Boletus aereus AC2266. The present invention also discloses the application of the Boletus aereus strain in the production of Boletus aereus. This strain has a relatively fast mycelial growth rate, a relatively low optimal growth temperature, a darker cap color, a thicker cap, and a yellower stipe, and is an excellent new variety that can be used for the industrial production of Boletus aereus.

The invention provides boletus nigrum G8 as well as a culture method and application thereof, and belongs to the technical field of edible mushrooms. The preservation number of the boletus nigrum G8 is CGMCC (China General Microbiological Culture Collection Center) No.20233. The invention also provides a corresponding culture method of the boletus nigrum G8 and application of the boletus nigrum G8 in culture of boletus nigrum mycorrhizal seedlings and potting. Compared with other strains, the boletus brownii G8 has the advantages that the growth speed is higher, and the obvious competitive advantage and application value are achieved; sporocarp can be formed in a short time through potting, and continuous mushroom production is achieved.

The present application belongs to the technical field of flavoring, and particularly relates to a black truffle composite flavoring and a preparation method thereof. The black truffle composite flavoring comprises embedded black truffle extract, which is prepared by embedding black truffle extract with an embedding agent. The black truffle extract is prepared by fermenting fresh black truffle with strains, wherein the strains comprise Lactobacillus acidophilus, Lactobacillus rhamnosus and Lactobacillus casei. In addition, the flavoring can further comprise other raw materials, such as one or more of boletus extract, edible salt, yeast extract, corn brewing sauce powder, corn starch, lactose, apple powder, lentinus edodes, pleurotus eryngii, grifola frondosa, laminaria japonica, lemon powder, wheat fermentation sauce, hericium erinaceus, cabbage powder, carrot powder, sophora flavescens powder and momordica grosvenori powder. The black truffle composite flavoring prepared by the present application has rich aroma and high storage stability, and is deeply loved by consumers.

The present invention belongs to the technical field of edible and medicinal fungi production, and specifically discloses an application of dark brown dichotoma boletus in producing cytochalasin E and a fermentation method for improving the yield. Specifically, the present invention provides a dark brown dichotoma boletus ( Phlebopus portentousus ) The application of YAFMF008 in the production of cytochalasin E, the present invention discovered that dark brown tricholoma boletus ( Phlebopus portentousus ) YAFMF008 can produce cytochalasin E, and the fermentation culture method has been optimized. The optimized culture medium can significantly increase the yield of cytochalasin E, providing a way to develop the efficient medicinal value of the dark brown boletus YAFMF008 and the efficient production of the bioactive ingredient cytochalasin E.

The present invention relates to the technical field of edible fungi. Specifically, it relates to a method for the secondary fruiting of Phlebopus portentosus. The method comprises the following steps: (1) raking the bacteria; (2) punching holes; (3) adding a nutrient supplement; (4) covering with soil; (5) fruiting management. The present invention can further transform and utilize the culture medium after the first fruiting, greatly improving the yield and reducing the production cost.

1. Name of the product in this design: Packaging Box (High-Fiber Bolete). 2. Intended use of this design: for food packaging. 3. The key design feature of this product is its pattern. 4. The picture or photo that best illustrates the key design points: Design 1 main view. 5. Design 1 is designated as the basic design.

The present invention belongs to the field of microbial technology and discloses a strain of Candida and its application in the fermentation of Yunnan Camellia. Candida was isolated from Yunnan wild edible fungi (white boletus) Candida railenensis )BTN‑HB‑M5, which was deposited in the Guangdong Microbial Culture Collection Center on September 20, 2024, with the deposit number GDMCC No: 65164. The Yunnan Camellia flower fermentation extract can be obtained by using Candida BTN‑HB‑M5 through appropriate culture and collection processes. The overall fermentation process is green and efficient. The Yunnan Camellia flower fermentation extract prepared is obtained by using only Candida ( Candida railenensis The fermented product of Camellia sinensis (Yunnan Camellia) can be used in food and daily chemical products. It maximizes the preservation of the natural active ingredients of Camellia sinensis, without any added ingredients or irritating byproducts, making it suitable for use in daily chemical products. This Camellia sinensis fermented extract has excellent antioxidant, anti-glycation, and anti-aging effects, significantly alleviating skin aging indicators through antioxidant activity, and has great application prospects in the field of daily chemical products.

The invention discloses a compound seasoning based on an in-situ enzymolysis and grease coating technology and a preparation method of the compound seasoning, and relates to the technical field of food processing and seasonings, the compound seasoning comprises fresh shiitake mushrooms, dried bolete, a yeast extract, onion powder, garlic powder, kelp powder, coconut oil and silicon dioxide, the seasoning takes the shiitake mushrooms and the bolete as main raw materials, and the seasoning is prepared by mixing the shiitake mushrooms, the dried bolete, the yeast extract, the onion powder, the garlic powder, the kelp powder, the coconut oil and the silicon dioxide. According to the invention, mushroom is taken as a raw material, natural components such as yeast extract and kelp powder are taken as auxiliary materials, guanylic acid and free amino acid in mushroom cells are efficiently released through a composite enzymatic hydrolysis technology, a multi-layer flavor system is constructed by combining a temperature-controlled and humidity-controlled Maillard reaction, and the stability and solubility are improved through microcapsule granulation. The product is completely free of sodium glutamate, I + G or synthetic essence, but the palatable taste strength can be 1.5-2 times of that of a traditional monosodium glutamate type product, and the product is suitable for the fields of healthy food, infant complementary food and plant-based food. The invention solves the technical bottleneck of insufficient delicate flavor of natural seasonings, and has obvious innovativeness and industrialization value.

The invention provides bovine liver mushroom and a molecular marker identification method thereof, and belongs to the technical field of breeding. The beef liver shiitake mushroom J3 is preserved in the China Center for Type Culture Collection on May 21, 2025, and the preservation number of the beef liver shiitake mushroom J3 is CCTCC NO: M 20251141. According to the method, the beef liver mushroom J3 with unique characters is successfully cultivated by combining a mushroom cross breeding technology with precise screening after fruiting. The sporocarp of the beef liver shiitake mushroom J3 inherits the advantage of high vigor of two parent hyphae, and obtains the characters different from the two parent sporocarp: the stem is thick, the cap is not easy to open, the meat quality is thick, the whole sporocarp looks like bolete without opening, the meat quality is fleshy, the taste is crisp and tender, the edibility of the sporocarp is high, and the development and application prospects are very good.

1. Name of the designed product: packing bag (high fiber boletus). 2. Use of the designed product: for food packing. 3. Design points of the designed product: in the pattern. 4. Picture or photo that best shows the design points: front view. 5. The designed product is a thin product, and the left view, right view, top view and bottom view are omitted.

The invention belongs to the technical field of microbial food fermentation engineering, and discloses umami soy sauce based on ion acceleration multi-strain graded fermentation and a preparation method of the umami soy sauce. According to the method, through step-by-step fermentation of boletus rubescens hyphae and thelephora ganbajun Zang hyphae, the problem that traditional soy sauce is insufficient in natural flavor is thoroughly solved, and the rich edible mushroom fresh fragrance is increased; original nutritional ingredients of the soy sauce are completely reserved through fermentation and enzymolysis of mycelia, and human body necessary nutrient substances such as glutamic acid, lysine and polyhydric alcohols are additionally supplemented; an ion regulation means is innovatively introduced, food-grade organic selenium and food-grade calcium carbonate are added in the fermentation stage of boletus rubescens hyphae, the fermentation time is shortened, and the production efficiency is improved. The technical path is not only suitable for soy sauce production, but also can be expanded to the production of umami products such as monosodium glutamate, umami powder and the like, a brand new upgrading direction is provided for the fermentation type seasoning industry, and the transformation of a traditional soy sauce process from homogeneous production to differentiation and nutrition is promoted.

The invention relates to the technical field of edible fungus cultivation, in particular to a method for accurately analyzing the matrix utilization rate of phlebopus portentosus, which comprises the following steps: performing conventional cultivation on phlebopus portentosus by using a cultivation matrix with grains as nutrient substances; after matrix samples at different cultivation stages are rubbed, grains are separated and dried, and shriveled grains are removed; randomly selecting 100 grains from the grain sample, and weighing to obtain the dry weight of 100 grains; 100 grain samples selected at different cultivation stages are hulled, the dry weight of 100 grains is calculated, and then the weight loss percentage of the rice grains and the accumulated weight loss percentage are calculated; and analyzing to obtain the utilization conditions of the phlebopus portentosus on the substrate in different cultivation stages. According to the method, the utilization condition of the matrix can be accurately analyzed in the culture process of the phlebopus portentosus, and timely and accurate adjustment reference data is provided for subsequent artificial cultivation of the phlebopus portentosus.

The present application relates to a boletus planting culture medium and a preparation method thereof, and belongs to the technical field of biology. The boletus planting culture medium is prepared by using bagasse fermented by Clostridium cellulolyticum, Halanaerobium sp. and Leuconostoc citreum as a culture medium raw material, and combining the bagasse with cottonseed hulls, bran, corn flour, gypsum powder, glucose, potassium dihydrogen phosphate and magnesium sulfate. The boletus cultivated by using the culture medium can effectively improve the mycelium growth of boletus, the time of boletus mushroom growth, the yield, the content of crude protein, polysaccharide and ergothioneine in the fruiting body, and the quality of boletus.

The utility model discloses a cultivation container for boletus aereus cultivation, which relates to the technical field of boletus aereus cultivation and comprises a shell, a plurality of planting frames distributed in an annular array are arranged in the shell, two symmetrically distributed lighting lamps are fixedly mounted on the upper surface in the shell, a swing pipeline is rotatably mounted on the upper portion of the shell, and the swing pipeline is communicated with the planting frames. A plurality of spraying heads distributed at equal intervals are fixedly installed on the lower portion of the swing pipeline, a driving frame is fixedly installed in the middle of the swing pipeline, a rotating disc is rotatably installed on the upper surface of the interior of the shell, a driving rod is slidably arranged on one side of the rotating disc in a penetrating mode, and one side of the driving rod is slidably clamped to the outer surface of one side of the driving frame; according to the device, when the boletus aereus is wetted with water, the swing pipeline can flexibly swing within a preset range, it is ensured that water mist can uniformly cover the whole culture medium layer, the water wetting efficiency is improved, and it is also ensured that the boletus aereus obtains sufficient and uniform water supply in the growth process.

The invention belongs to the technical field of microorganisms, and discloses candida and application thereof in fermentation of camellia yunnanensis. Candida ralensis BTN-HB-M5 is obtained by separating wild edible fungi (white boletus) in Yunnan, the candidida ralensis BTN-HB-M5 is preserved in Guangdong Microbial Culture Collection Center on September 20, 2024, and the preservation number is GDMCC No: 65164. The camellia yunnanensis fermentation extract can be obtained by utilizing the candida sp. BTN-HB-M5 through proper culture and collection processes, and the whole fermentation process is green and efficient; the prepared camellia japonica fermentation extract is a product fermented only by using Candida railensis, can be used in food and daily chemical products, keeps natural active substances of camellia japonica to the greatest extent, is free of other additional components and irritant by-products, and can be applied to the daily chemical products. The camellia yunnanensis fermentation extract has excellent anti-oxidation, anti-saccharification and anti-aging effects, can obviously relieve skin aging indexes through oxidation resistance, and has a great application prospect in the field of daily chemical products.

The application discloses a brown ring lactarius xylolipalm and a preparation method and application thereof. The brown ring lactarius xylolipalm is xylolipalm formed by taking alpha-(1→4)-xylose as a main chain, alpha-(1→)-glucose as a branch chain and a palmitic acid molecule through an ester bond. The application further discloses a preparation method of the brown ring lactarius xylolipalm and an application thereof in immune regulation, and the brown ring lactarius xylolipalm can be used as a dietary supplement or a food additive for immunodeficiency patients or tumor patients.

The invention discloses burkholderia cepacia and application thereof in promoting growth of edible mushrooms, and belongs to the technical field of microorganisms. According to the invention, a growth-promoting strain is separated and screened from an earthing material of phlebopus portentosus, the form, physiological and biochemical characteristics and 16S rDNA sequence of the growth-promoting strain are measured, and the strain is identified as Burkholderia cepacia. Experiments prove that the burkholderia cepacia disclosed by the invention can obviously promote the development of sporocarp of the phlebopus portentosus, and can play a role in improving the efficiency and improving the quality of the production of the phlebopus portentosus, so that the burkholderia cepacia has a relatively good application prospect.