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Method for determining bioavailability and bioavailability of cadmium in boletus cinereus

A bioavailability, bolete technology, applied in the direction of bacteria, color/spectral property measurement, resistance to vector-borne diseases, etc. good effect

Pending Publication Date: 2022-07-08
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For dietary toxic and hazardous substances, the NOAEL value obtained in this way may cause significant biological effectiveness of the test substance between animal models and human diet due to physiological differences between animals and humans, and differences between exposure methods and normal diets. sexual difference

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  • Method for determining bioavailability and bioavailability of cadmium in boletus cinereus

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Embodiment 1

[0035] Example 1: as figure 1 As shown, the present embodiment utilizes the in vitro full biomimetic digestion and absorption method to measure the bioavailability and bioavailability of cadmium in Boletus boletus as follows:

[0036] Gray-brown boletus dry powder: The gray-brown boletus collected from various places is dried, crushed, and passed through a 40-mesh sieve by conventional drying methods, and the material under the sieve is taken. After microwave digestion-graphite furnace atomic absorption spectrometry, the cadmium content varies, and three samples of Boletus taupe with different cadmium content were selected (low: 7.31mg / kgDW; medium: 24.20mg / kgDW; high: 41.64mg / kgDW) for the following implementation process.

[0037] 1. Stomach physical and chemical digestion process: Accurately weigh 3g dry powder of boletus taupe into a 100mL anaerobic bottle, add 27mL deionized water, swell and rehydrate at room temperature. Add 10 mL of commercially available artificial...

Embodiment 2

[0050] Embodiment 2: At the same time in this embodiment, the standard substance GBW10025 (GSB-16) Spirulina is used for the recovery rate of cadmium in the detection method and the repeatability of the measurement results, and the steps are as follows:

[0051] The standard material for biological component analysis GBW10025 (GSB-16) Spirulina (developed by: Institute of Geophysical and Geochemical Exploration, Chinese Academy of Geological Sciences) was purchased, and the standard value of cadmium content was 0.37±0.03mg / kgDW.

[0052] 1. Stomach physical and chemical digestion process: Accurately weigh 3g of spirulina powder into a 100mL anaerobic bottle, add 27mL of deionized water, and swell and rehydrate at room temperature. Add 10mL of commercially available artificial gastric juice (Shanghai Yuanye Biotechnology Co., Ltd., product number: R41110), in a solution containing 10% CO 2 In the anaerobic box of 37℃, 100rpm water bath shaking for 1h. Pipette 4 mL of the mixtu...

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Abstract

The invention provides an in-vitro full-bionic digestion and absorption method for determining the bio-accessibility and bio-availability of a toxic pollutant cadmium in boletus griseus, and belongs to the field of ecological toxicology research. Full-bionic digestion and absorption in the method comprises the following three steps: (1) establishing an in-vitro stomach and small intestine bionic digestion process, and evaluating stomach and small intestine biological accessibility of cadmium in boletus cinereus; (2) establishing an in-vitro coliform fermentation process, and evaluating the large intestine biological accessibility of cadmium in boletus cinereus; and (3) establishing a Caco-2 single cell layer bionic intestinal absorption process, and evaluating the bioavailability of cadmium in boletus cinereus. The method provided by the invention provides a more rigorous data basis for health risk assessment of the toxic pollutant cadmium in boletus cinereus, and can support improvement and perfection of a method for formulating a limit standard of toxic and harmful substances in food.

Description

technical field [0001] The invention relates to the technical field of ecotoxicology research, in particular to a method for determining the bioavailability and bioavailability of cadmium in Boletus boletus. Background technique [0002] Compared with vascular plants, macrofungi have a stronger ability to absorb heavy metals in the growth environment, and can bioaccumulate typical toxic and harmful mineral elements such as cadmium, lead, mercury, and arsenic in their fruiting bodies. Cultivation of edible fungi controls the pollution of harmful minerals in products by selecting fine varieties, improving the production environment, and reforming cultivation techniques, so as to ensure food safety and the development of the edible fungi industry. Compared with cultivated edible fungi, wild edible fungi have a variety of species, complex growth environment, poor controllability of growth process, and serious pollution of harmful minerals. Yunnan Province is an important wild e...

Claims

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Application Information

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IPC IPC(8): G01N21/31C12N1/20
CPCG01N21/3103C12N1/20Y02A50/30
Inventor 庄永亮张婷婷谭俊杰孙丽平
Owner KUNMING UNIV OF SCI & TECH
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