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Boletus and application thereof
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A technology of boletus and mother species, which is applied in the field of boletus fungi and its application, can solve the problems of heavy soil odor, affecting the appearance of dishes, and restricting the development of boletus artificial cultivation industry, etc.
Active Publication Date: 2021-06-22
郑永彬
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Black beef liver has a heavy earthy smell, which is difficult for some consumers to accept, and it will brown quickly after cooking, which will affect the appearance of dishes, which largely limits the development of boletus artificial cultivation industry
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Embodiment 1
[0056] Embodiment 1, the isolation and identification of boletus
[0057] 1. Separation
[0058] 1. Collection
[0059] From December 30, 2019 to April 6, 2020, Yueda Village, Datian Town, Dongfang City, Hainan Province, China, at an altitude of 30-63 meters, a habitat near latitude 19°12′33″N and longitude 108°53′58″E , Collected eleven samples of wild fruiting bodies successively. The habitat is the sandy soil layer in tropical and subtropical grasses dominated by Rosaceae leguminous plants.
[0064] Fresh, tender, pest-free fruiting bodies (called wild boletus, such as figure 1 shown) together with the base soil into the sample bag and brought back to the laboratory. The interva...
Embodiment 2
[0107] Example 2, artificial cultivation of boletus CCTCC No: M2020221
[0108] 1. Exploration of artificial cultivation conditions
[0109] (1) Raw material and culture medium for testing
[0110] The sawdust, cottonseed husks, miscellaneous sawdust, wheat bran, corn flour, and light calciumcarbonate in the cultivation material are all purchased from conventional commercial channels in the local market.
[0111] Tested strain: CCTCC No: M2020221 that has been preserved. Unless otherwise specified, the parent species used in this example are all obtained from the CCTCC No: M2020221 that has been patent preserved.
[0112] Potato agar medium (PDA medium): 200g of potatoes (peeled), 20g of glucose, 18-22g of agar, 1000mL of water, natural pH; the preparation method is conventional.
[0113] Carbon source test basal medium: peptone 3g, KH 2 PO 4 1.5g, MgSO 4 0.5g, 20g agar, add water 1000mL, pH natural.
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Abstract
The invention discloses boletus and application thereof. The invention provides Buchwaldoboletus sp. RGHUNG201903181D85 with a preservation number of CCTCC No: M2020221. The invention also provides a method for artificially cultivating the Buchwaldoboletus sp.. The method comprises the following steps of 1) carrying out mother strain culture on the Buchwaldoboletus sp. according to claim 1 in a PDA enriched solid culture medium to obtain a mother strain; 2) transferring the mother strain into a PDA enriched liquid culture medium for stock culture to obtain a stock; 3) transferring the stock into a cultivation material for cultivating a cultivar to obtain the cultivar; and 4) transferring the cultivar into a cultivation material for mycelium culture and fruiting in sequence to obtain the artificially cultivated Buchwaldoboletus sp.. According to the invention, a new Buchwaldoboletus sp. species is discovered; the fruiting bodies are generally medium to large and are single or scattered; the mushroom stems are thick and long; the structure is solid; the texture is crisp and tender; the taste is soft, smooth and refreshing; and the Buchwaldoboletus sp. species is a new edible mushroom species with good development prospect and can be used for artificial cultivation.
Description
technical field [0001] The invention belongs to the field of biotechnology, relates to the field of edible fungi, in particular to a boletusfungus and its application. Background technique [0002] Boletaceae fungi are an important group of macrobasidiomycetes. They have a high status among macrofungi and are one of the most concerned species. According to Kir's classification method, the family includes 35 genera and more than 400 species. [0003] Boletus has important economic value and ecological value. Edible boletus is delicious, nutritious, and contains a variety of amino acids, minerals and other active substances. The role of prevention and treatment of colds. [0004] Since boletus is mostly mycorrhizal, it is difficult to leave the host tree for artificial cultivation. At present, some scientific research institutions are doing research on the artificial cultivation of boletus, but they are all in the laboratory stage. The only known commercial artificial cu...
Claims
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