Method for manufacturing edible mushroom solid liquefied strains

Abstract

The invention relates to the field of mushroom cultivation technologies, in particular to a method for manufacturing edible mushroom solid liquefied strains. The method comprises the steps that first-level liquid strains are inoculated into a culture flask, and liquid spawn is obtained; a solid cultivation material is prepared, the liquid spawn is cultured in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, cultivation is conducted in a dark place, the cultivation humidity ranges from 50% to 60%, and the strains can grow full of the strain bottles after being cultured by 20-30 days; the strain bottles filled with the strains and having no infectious microbe are selected, and the strains are crushed in a pulverizer and inoculated into sterile water for direct production and inoculation. The method for manufacturing the edible mushroom solid liquefied strains has the advantages that the strain running speed is high and is 1 / 3 higher than that of solid strain running, 3-4 times of expansion inoculation are not needed, the strain contamination problem caused in the expansion inoculation process is solved, and time needed by expanding propagation is saved; growing points are more than the growing points of common liquid strains, strain running is uniform, the strain ages are consistent, produced mushrooms are orderly and good in quality, and fruit body yield can be improved by 20%-30%; sterile water is adopted for liquefying mycelia, molds and other infectious microbes can not grow, and contamination can be reduced by 5%-10%.

Description

technical field

[0001] The invention relates to the field of mushroom cultivation techniques, in particular to a method for preparing solid liquefied strains of edible mushrooms. Background technique

[0002] At present, edible fungus strains are divided into two types: solid strains and liquid strains. Solid strains are easy to operate and easy to observe, and can effectively prevent the introduction of contaminated strains. However, solid strains also have the characteristics of slow germination, low yield, and Mushrooms are not neat, and they are easy to be infected with miscellaneous bacteria during the fungus stage. The traditional liquid strains are used to produce first-level strains on a shaking table, which needs to be expanded 3-4 times, and then used for inoculation. Using this liquid strain can effectively speed up the growth of bacteria, but the operation is complicated and it is easy to cause large batches of contamination. question. Contents of the inventio...

Examples

Embodiment 1

[0021] Embodiment 1: oyster mushroom

[0022] First, prepare the oyster mushroom strain,

[0023] The preparation method of flat mushroom solid liquefaction strain comprises the following steps:

[0024] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, wi...

Embodiment 2

[0029] Embodiment 2: Jiji mushroom

[0030] First, prepare the jitake mushroom strain,

[0031] The preparation method of the solid liquefied bacterial strain of Jiji mushroom comprises the following steps:

[0032] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shak...

Embodiment 3

[0037] Embodiment 3: Xiuzhen mushroom

[0038] The preparation method of the solid liquefied bacterial strain of xiuzhen mushroom comprises the following steps:

[0039]Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, with 200-300ml of culture material in ...

technical field

[0001] The invention relates to the field of mushroom cultivation techniques, in particular to a method for preparing solid liquefied strains of edible mushrooms. Background technique

[0002] At present, edible fungus strains are divided into two types: solid strains and liquid strains. Solid strains are easy to operate and easy to observe, and can effectively prevent the introduction of contaminated strains. However, solid strains also have the characteristics of slow germination, low yield, and Mushrooms are not neat, and they are easy to be infected with miscellaneous bacteria during the fungus stage. The traditional liquid strains are used to produce first-level strains on a shaking table, which needs to be expanded 3-4 times, and then used for inoculation. Using this liquid strain can effectively speed up the growth of bacteria, but the operation is complicated and it is easy to cause large batches of contamination. question. Contents of the inventio...

Examples

Embodiment 1

[0021] Embodiment 1: oyster mushroom

[0022] First, prepare the oyster mushroom strain,

[0023] The preparation method of flat mushroom solid liquefaction strain comprises the following steps:

[0024] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, wi...

Embodiment 2

[0029] Embodiment 2: Jiji mushroom

[0030] First, prepare the jitake mushroom strain,

[0031] The preparation method of the solid liquefied bacterial strain of Jiji mushroom comprises the following steps:

[0032] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shak...

Embodiment 3

[0037] Embodiment 3: Xiuzhen mushroom

[0038] The preparation method of the solid liquefied bacterial strain of xiuzhen mushroom comprises the following steps:

[0039]Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, with 200-300ml of culture material in ...