Method for manufacturing edible mushroom solid liquefied strains

A technology for solid liquefaction strains and production methods, which is applied in the directions of botanical equipment and methods, applications, fungi, etc., can solve the problems of slow growth of solid strains, irregular fruiting, and large-scale pollution, and achieves the development of bacteria. Consistent, tidy fruiting, reducing the effect of bacterial contamination

Inactive Publication Date: 2015-06-24
青岛联合菌业科技发展有限公司
View PDF9 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, edible fungus strains are divided into two types: solid strains and liquid strains. Solid strains are easy to operate and easy to observe, and can effectively prevent the introduction of contaminated strains. However, solid strains also have the characteristics of slow germination, low yield, and Mushrooms are not neat and easy to be infected with miscellaneous bacteria during the fungal stage
The traditional liquid strains are used to produce first-level strains on a shaking table, which needs to be expanded 3-4 times, and then used for inoculation. Using this liquid strain can effectively speed up the growth of bacteria, but the operation is complicated and it is easy to cause large batches of contamination. question

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: oyster mushroom

[0022] First, prepare the oyster mushroom strain,

[0023] The preparation method of flat mushroom solid liquefaction strain comprises the following steps:

[0024] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, wi...

Embodiment 2

[0029] Embodiment 2: Jiji mushroom

[0030] First, prepare the jitake mushroom strain,

[0031] The preparation method of the solid liquefied bacterial strain of Jiji mushroom comprises the following steps:

[0032] Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shak...

Embodiment 3

[0037] Embodiment 3: Xiuzhen mushroom

[0038] The preparation method of the solid liquefied bacterial strain of xiuzhen mushroom comprises the following steps:

[0039]Step 1: Inoculate the first-level liquid bacteria (sterilized by ultraviolet light for 20 minutes before inoculation, inoculate in the ultra-clean workbench, inoculate 4-5 pieces of bacteria with an area of ​​3mm*3mm in each bottle) in culture bottles, 22- Cultivate statically at 25°C for 2-3 days, the growth of the bacterial block is normal, adjust the rotating speed of the oscillator to 200r / min under the condition of no bacteria, and cultivate for 5-10 days to obtain the liquid species; the liquid species described in step 1 The culture medium is: 1L culture material formula is: 200g of potatoes, 20g of bran, 20g of glucose, 1g of potassium dihydrogen phosphate, and 0.5g of magnesium sulfate. After the culture solution is boiled, it is divided into 1000ml shake flasks, with 200-300ml of culture material in ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to the field of mushroom cultivation technologies, in particular to a method for manufacturing edible mushroom solid liquefied strains. The method comprises the steps that first-level liquid strains are inoculated into a culture flask, and liquid spawn is obtained; a solid cultivation material is prepared, the liquid spawn is cultured in strain bottles, the cultivation temperature ranges from 22 DEG C to 25 DEG C, cultivation is conducted in a dark place, the cultivation humidity ranges from 50% to 60%, and the strains can grow full of the strain bottles after being cultured by 20-30 days; the strain bottles filled with the strains and having no infectious microbe are selected, and the strains are crushed in a pulverizer and inoculated into sterile water for direct production and inoculation. The method for manufacturing the edible mushroom solid liquefied strains has the advantages that the strain running speed is high and is 1/3 higher than that of solid strain running, 3-4 times of expansion inoculation are not needed, the strain contamination problem caused in the expansion inoculation process is solved, and time needed by expanding propagation is saved; growing points are more than the growing points of common liquid strains, strain running is uniform, the strain ages are consistent, produced mushrooms are orderly and good in quality, and fruit body yield can be improved by 20%-30%; sterile water is adopted for liquefying mycelia, molds and other infectious microbes can not grow, and contamination can be reduced by 5%-10%.

Description

technical field [0001] The invention relates to the field of mushroom cultivation techniques, in particular to a method for preparing solid liquefied strains of edible mushrooms. Background technique [0002] At present, edible fungus strains are divided into two types: solid strains and liquid strains. Solid strains are easy to operate and easy to observe, and can effectively prevent the introduction of contaminated strains. However, solid strains also have the characteristics of slow germination, low yield, and Mushrooms are not neat, and they are easy to be infected with miscellaneous bacteria during the fungus stage. The traditional liquid strains are used to produce first-level strains on a shaking table, which needs to be expanded 3-4 times, and then used for inoculation. Using this liquid strain can effectively speed up the growth of bacteria, but the operation is complicated and it is easy to cause large batches of contamination. question. Contents of the inventio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04C05G3/00
CPCC12N1/14
Inventor 陈翠翠巩玉辉宋芳芳周绍辉隋海周陈燕于鄂
Owner 青岛联合菌业科技发展有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap