Composite microbial inoculant containing bacillus subtilis and application of composite microbial inoculant in degrading straws at low temperature

Abstract

The invention provides a composite microbial inoculant containing bacillus subtilis, the composite microbial inoculant is composed of six strains, the decomposition days of rice straw can be increasedby 2-3 days in an environment with the outdoor temperature of 1-6 DEG C, and the composite microbial inoculant is especially suitable for northern areas. The composite microbial inoculant is mixed with rice straw, the tomato planting effect is good, the tomato plants can grow more vigorously even at the room temperature of about 10 DEG C compared with a control group without the composite microbial inoculant, the harvest time is advanced by 14 days, the yield is increased by 607 kg / mu, and the occurrence rate of diseases and insect pests is reduced by 7.1%. A large amount of bacillus is added, so that harmful microorganisms such as vibrio, escherichia coli and baculovirus in soil can be effectively inhibited, and the very strong effect of purifying the soil is achieved. The composite microbial inoculant provided by the invention has an obvious yield-increasing effect as an auxiliary fertilizer, can effectively enhance the resistance of tomatoes to diseases and insect pests, and improves the quality of tomatoes.

Description

technical field

[0001] The invention relates to the field of microorganisms, in particular to a composite bacterium containing Bacillus subtilis and its application in degrading straw at low temperature. Background technique

[0002] Rice straw, or rice straw, is a rather distinctive economic by-product with many uses. In addition to being used for livestock such as cattle and sheep, straw ropes, straw sandals and coir raincoats made of straw are essential items for many farmers. But the best way is to bury the straw on the spot, which can strengthen the nutrients of the soil in addition to not causing air pollution. According to research conducted by the Taiwan Agricultural Improvement Association, burying rice straw on the spot in rice fields can increase rice production, and at the same time, the content of phosphorus, potassium, calcium, iron, and silicon in the fields will also increase.

[0003] Due to the long-term low temperature in the north, rice straw is not eas...

Examples

Embodiment 1

[0020] The mutagenic screening of embodiment 1 verrucous Myromyces

[0021] (1) Morphological identification and screening

[0022] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0023] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0024] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0030] The low-temperature acclimatization of embodiment 2 bacterial strains

[0031] (1) Enrichment culture strains

[0032] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L, enrichment culture for 4 generations.

[0033] (2) Preliminary screening and domestication of low-temperature cellulose-degrading composite strains

[0034] Using the filter paper disintegration method, the above-mentioned enriched culture was inoculated into the filter paper strip medium to...

Embodiment 3

[0045] The preparation of embodiment 3 composite bacterial agents

[0046] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Bacillus subtilis and Bacillus subtilis were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial liquid reached 2.0×10 8 individual / mL.

[0047] Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 10% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% of Bacillus plasmodia, 10% of Manganese peroxidase-producing Layseia lanceolata, and 30% of Bacillus subtilis, fully stirred and mixed to obtain a mixed bacterial liquid.

technical field

[0001] The invention relates to the field of microorganisms, in particular to a composite bacterium containing Bacillus subtilis and its application in degrading straw at low temperature. Background technique

[0002] Rice straw, or rice straw, is a rather distinctive economic by-product with many uses. In addition to being used for livestock such as cattle and sheep, straw ropes, straw sandals and coir raincoats made of straw are essential items for many farmers. But the best way is to bury the straw on the spot, which can strengthen the nutrients of the soil in addition to not causing air pollution. According to research conducted by the Taiwan Agricultural Improvement Association, burying rice straw on the spot in rice fields can increase rice production, and at the same time, the content of phosphorus, potassium, calcium, iron, and silicon in the fields will also increase.

[0003] Due to the long-term low temperature in the north, rice straw is not eas...

Examples

Embodiment 1

[0020] The mutagenic screening of embodiment 1 verrucous Myromyces

[0021] (1) Morphological identification and screening

[0022] The soil in the forest area of ​​Changbai Mountain Nature Reserve was collected and cultivated on PDA medium by conventional methods. The mycelium of the strain was white flocculent at first, and grew divergently towards the periphery. After 8 days of culture, the color of conidia continued to deepen, and glue dots appeared; after 10 days of culture, the colony was in the shape of concentric rings, the conidia showed black glue spots, and the back of the colony appeared Hazel radial folds.

[0023] Use a puncher to get a 1cm-diameter bacterial block, inoculate it on an aniline blue selective medium, and culture it in an aerobic incubator at 30°C for 10 days, observe the fading of aniline blue, and select a strain with a fast fading rate and strong ability as candidate strains.

[0024] At the same time, in order to directional screen lignin-deg...

Embodiment 2

[0030] The low-temperature acclimatization of embodiment 2 bacterial strains

[0031] (1) Enrichment culture strains

[0032] Inoculate the strain into a Erlenmeyer flask filled with 150mL of PDA medium, add filter paper pieces with a length of 5cm and a width of 2cm, incubate at a constant temperature at 30°C for 1 hour and mix evenly, and make 10 -1 -10 -9 For dilution, 1 mL of each dilution was inoculated in PDA medium with filter paper as the only carbon source, and static enrichment culture was carried out at 28°C for 4 generations. Screen out the materials with short breakage time and high degree of ulceration of filter paper. PDA medium: potato 200g / L, glucose 20g / L, agar 15g / L, enrichment culture for 4 generations.

[0033] (2) Preliminary screening and domestication of low-temperature cellulose-degrading composite strains

[0034] Using the filter paper disintegration method, the above-mentioned enriched culture was inoculated into the filter paper strip medium to...

Embodiment 3

[0045] The preparation of embodiment 3 composite bacterial agents

[0046] Cellulase-producing Paenibacillus, xylanase-producing Alternaria, laccase-producing Mycobacterium verrucosus, lignin-peroxidase-producing Bacillus colloid, manganese-peroxidase-producing Lys Bacillus subtilis and Bacillus subtilis were activated in a conventional manner and cultured until the number of viable bacteria in the bacterial liquid reached 2.0×10 8 individual / mL.

[0047] Mix the bacterial solution according to the following mass ratio: 20% of Paenibacillus cellulase-producing bacteria, 10% of Alternaria xylanase-producing bacteria, 20% of Laccase-producing Mycobacterium verrucosus, lignin peroxidase glue 10% of Bacillus plasmodia, 10% of Manganese peroxidase-producing Layseia lanceolata, and 30% of Bacillus subtilis, fully stirred and mixed to obtain a mixed bacterial liquid.

Images