Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress

A technology of suspension cells and Luo Han Guo, applied in plant cells, fermentation, etc., to achieve the effects of increased biomass, increased yield, and higher specific growth rate of cells

Active Publication Date: 2018-07-06
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At home and abroad, a lot of research has been done on the physiological, biochemical, and molecular biological response mechanisms o

Method used

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  • Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress
  • Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress
  • Method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress

Examples

Experimental program
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Example Embodiment

[0032] Example 1

[0033] Step 1: Take the mature embryos of Luo Han Guo and place them in B5 containing sucrose 25g / L, 6-benzylamino adenine 0.05mg / L, naphthalene acetic acid 2mg / L, inositol 50mg / L, agar 4g / L, pH 5.9 Subculture on solid medium, select fresh embryogenic callus that are subcultured three times in succession and have vigorous growth, loose texture, and stable and uniform state; Step 2: Inoculate the fresh embryogenic callus obtained in Step 1 to 25g / L was transferred to B5 liquid medium containing sucrose 20g / L, 6-benzylaminoadenine 0.05mg / L, naphthaleneacetic acid 3mg / L, inositol 50mg / L, pH 5.9, and rotating at 80r / L Min, the culture temperature is 24 ℃, culture in a dark shaker to obtain the primary generation of Luo Han Guo suspension cell system.

[0034] Step 3: Use the primary Luo Han Guo suspension cell system obtained in Step 2 to cultivate mature Luo Han Guo suspension cells; the ratio of potassium to sodium in the original medium is 25:1, the potassium io...

Example Embodiment

[0037] Example 2

[0038] Step 1: Take the seed embryo of Momordica grosvenori and place it in B5 solid containing 35g / L of sucrose, 0.15mg / L of 6-benzylamino adenine, 6mg / L of naphthaleneacetic acid, 150mg / L of inositol, 6g / L of agar, and pH 6.0 Subculture on the medium, and select embryogenic callus subcultured 5 times in succession;

[0039] Step 2: Transfer the embryogenic callus obtained in Step 1 with an inoculation amount of 75g / L to containing 40g / L of sucrose, 0.15mg / L of 6-benzylamino adenine, 5mg / L of naphthaleneacetic acid, and 150mg / L of inositol , B5 liquid medium with pH 6.0, and in a dark shaker with a rotation speed of 150r / min, a culture temperature of 26°C, and a primary generation Luo Han Guo suspension cell system.

[0040] Step 3: Use the first generation Luo Han Guo suspension cell system obtained in Step 2 to cultivate mature Luo Han Guo suspension cells; the ratio of potassium to sodium in the original medium is 25:1, the potassium ion concentration is contr...

Example Embodiment

[0043] Example 3

[0044] Step 1: Take the mature embryos of Momordica grosvenori, which contains 30g / L of sucrose, 0.1mg / L of 6-benzylamino adenine, 4.0mg / L of naphthaleneacetic acid, 100mg / L of inositol, 5.0g / L of agar, and the pH range is Subculture on 5.9 B5 solid medium, select fresh embryogenic callus that are subcultured 4 times in succession and have vigorous growth, loose texture, stable and uniform state;

[0045] Step 2: Transfer the fresh embryogenic callus obtained in Step 1 with an inoculum amount of 50g / L to 30g / L 6-benzylamino adenine 0.1mg / L, 4.0mg / L naphthaleneacetic acid, and 100mg inositol. / L, pH range of 5.9 B5 liquid medium, and in the rotation speed of 110r / min, culture temperature of 25 ℃, dark shaker to obtain the first generation of Luo Han Guo suspension cell system;

[0046] Step 3: Use the first generation Luo Han Guo suspension cell system obtained in Step 2 to cultivate mature Luo Han Guo suspension cells; the ratio of potassium to sodium in the origi...

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Abstract

The invention relates to a method for promoting suspension cell growth of momordica grosvenori and improving content of mogroside V through salt stress. The method is characterized by comprising the steps: controlling a potassium ion concentration to be 100-2000 mg/kg in a process of culturing mature momordica grosvenori suspension cells by a primary momordica grosvenori suspension cell system, wherein a potassium-sodium ion concentration ratio is (5-20):1. According to the method disclosed by the invention, the growth amount and the specific growth rate of momordica grosvenori and the yield and synthesis rate of the mogroside V are improved, and momordica grosvenori suspension cell growth and large-scale market production of the mogroside V are facilitated; meanwhile, the production and culture cycle of cells is effectively shortened, the biomass of the momordica grosvenori suspension cells and the production process of the mogroside V are regulated in a manual manner, and quality control and management of the product can be facilitated.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for promoting the growth of monk fruit suspension cells and increasing the content of sweet glycoside V through salt stress. Background technique [0002] Mogroside V, also known as mogroside, is the main sweet component of Luo Han Guo in Cucurbitaceae, and its sweetness is 350 times that of sucrose. Currently, seven monomer components have been identified, among which mogroside V and Sai The sweetness of phylloside Ⅰ is the strongest. Mogroside Ⅴ has medicinal effects such as clearing away heat and moistening the lungs, soothing the throat, relaxing the bowels, etc. At the same time, Mogroside Ⅴ also has the characteristics of novel taste and pure taste, which makes it deeply loved by people. It is the most suitable sweetener and health care product for special groups such as patients with diabetes, high blood pressure, and diabetes, and has a wide range of application val...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12P33/00
CPCC12N5/04C12P33/00
Inventor 郭美锦宋云飞陈雨霞王泽建李杰杨文国庄英萍储炬肖慈英
Owner EAST CHINA UNIV OF SCI & TECH
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