16 results about "Cell biomass" patented technology

The present invention relates to a cell biomass (e.g. food product or pet food product) or its components prepared from cultivated metazoan cells, method of its preparation and a system for its preparation.

The present invention provides a cocoa powder including at least one compound selected from the group consisting of: an essential amino acid, a sugar, a neurotransmitter, and any combination thereof, wherein the at least one compound is enriched in a biomass of Theobroma cacao cells compared to a control cocoa powder, and is derived therefrom.

The invention discloses a culture method for increasing the flavonoid content of suspension cells of codonopsis pilosula. The method comprises the following steps: (1) obtaining aseptic seedlings: disinfecting codonopsis pilosula seeds, inoculating the disinfected codonopsis pilosula seeds into an MS culture medium, and culturing to obtain the aseptic seedlings; (2) callus induction and subculture: taking the roots of the aseptic seedlings as explants, and performing induction and subculture on an MS culture medium containing 2, 4-D, 6-BA and KT to obtain loose calluses; (3) suspension culture: inoculating the callus into an MS liquid culture medium containing NAA, 6-BA and KT for suspension culture to obtain high-biomass codonopsis pilosula suspension cells; and (4) adding an elicitor / precursor: adding methyl jasmonate (MeJA) or sodium acetate (NaAC) into the suspension culture system, and inducing flavone synthesis and accumulation. By optimizing a plant growth regulator composition in the culture medium, the biomass of the suspension cells of the codonopsis pilosula is remarkably improved (the highest biomass reaches 57.57 g / L); by adding MeJA or NaAC, the daily average yield of flavone is increased to 16.69 mg / L / d, and the daily average yield of flavone is increased to 18.20 mg / L / d. According to the method, the culture period is about 70 days, and a feasible way is provided for efficient and large-scale production of codonopsis pilosula flavone.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing L-ornithine as well as a construction method and application of the recombinant corynebacterium glutamicum for producing L-ornithine, corynebacterium glutamicum SO30 is taken as an original strain, a SigD gene and a FruR gene are sequentially knocked out through a suicide plasmid pK18mobsacB mediated homologous recombination technology, and double knockout recombinant corynebacterium glutamicum SO30-SigD-FruR is constructed. The L-ornithine yield of the strain subjected to shake-flask fermentation reaches 51.88 g / L and is increased by 56.7% compared with that of an original strain SO30; the fermentation yield of a 5L bioreactor reaches 123 g / L and is increased by 57.6% compared with that of an original strain SO30, and the cell biomass and the sugar conversion rate are remarkably increased. Through global transcriptional regulation factor transformation, the existing yield bottleneck is broken through, and efficient strains and technical support are provided for industrial fermentation production of L-ornithine.

The present invention relates to a novel fermentation process using a particular strain of Kluyveromyces marxianus, referred to as KMPROTEO 1, to obtain a single-cell protein useful in the food industry.In the present description, the invention discloses a process for fermenting dextrose and other consumables to obtain a single-cell biomass. The disclosed process enables a high-protein product to be obtained that is in the range of 50 to 60% for use in the food industry. The disclosed process uses dextrose as a propagation medium for use by Kluyveromyces marxianus to obtain a single-cell biomass and, subsequently, a single-cell protein.

The present disclosure relates to a simple, economical and energy efficient process for production of fermentation product. The process of the present disclosure employs nutrient composition obtained from cell biomass of fermentation process. The process of the present disclosure provides for efficient production of fermentation product when compared to conventional process. The present disclosure also relates to a nutrient composition extracted from cell biomass obtained from fermentation process.

This invention relates to a method for preparing graphene-like materials based on microalgal cell biomass. The method includes the following steps: (1) pre-activating Spirulina platensis cells, drying the powder, then washing and grinding it; (2) pre-pyrolyzing the Spirulina platensis cell powder ground in step (1), followed by pre-pyrolysis and grinding; (3) secondary activation and pyrolysis of the microalgal cells: mixing the powder product obtained in step (2) with one or more of FeCl2·4H2O, FeCl3·6H2O, CaCl2·2H2O, and ZnCl2 in a mass ratio of 4:1 to 1:10, and performing secondary activation and pyrolysis; (4) post-processing to obtain graphene-like materials. This invention uses Spirulina platensis as biomass raw material and prepares graphene-like carbon materials with different morphologies, specific surface areas, and porosity through a pre-pyrolysis-secondary activation and pyrolysis process.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

The present application belongs to the field of biotechnology, and specifically relates to a method for expressing human serum albumin by using Nicotiana sylvestris NS suspension cell line, which comprises the following steps: establishing a stably cultured Nicotiana sylvestris suspension cell system, and producing by amplification, and the obtained cell biomass can reach up to 500 g / L. Then, taking human serum albumin as the research object, transformation screening is carried out in the Nicotiana sylvestris NS suspension cell system to realize high expression of HSA. The present application removes the complex tissue regeneration step, and through plant codon optimization and subcellular targeting strategy design, aims to overcome the problem of low expression of exogenous genes in plants, so as to realize efficient enrichment of target proteins in specific organelles (such as vacuole / endoplasmic reticulum, etc.). The technical scheme of the present application can be popularized and applied to the expression and screening of other types of functional proteins, and provides important technical reference and practical support for the application of plant suspension cell lines in the field of recombinant protein expression.

A synthetic meat product for human and animal consumption and methods for producing such food product are disclosed. The synthetic food product comprises or essentially consists of a cell biomass of avian cells grown in vitro in a chemically-defined serum free culture medium under controlled conditions and do not contain ant hazard contaminations.

The invention discloses application of cationic polymer compounded bentonite flocculation for harvesting wide-salinity microalgae and a harvesting method, and belongs to the technical field of environmental protection. The method comprises the following steps: adding a polydimethyldiallylammonium chloride solution and a sodium bentonite suspension into an algae solution according to the cell biomass of microalgae to be harvested, stirring, settling and collecting to finish harvesting. According to the method disclosed by the invention, the microalgae in the wide-salt environment are harvested by combining PDDAC and BE for flocculation, the microalgae can be efficiently flocculated and harvested within the range of 0-40% of water salinity, and the harvesting rate can reach 90% or above; the flocculating efficiency is high, the dosage of a flocculating agent is small, the floc is large in size and compact, and the salt-resistant characteristic is remarkable; the method is suitable for efficient harvesting of microalgae in artificial culture or natural water under a wide salinity condition, provides a key technology for the field of microalgae harvesting, and has a wide application prospect; the problems of poor salinity tolerance of a flocculating agent, large dosage of the flocculating agent and poor harvesting effect in microalgae harvesting through flocculation at present are solved.

This invention relates to cell biomass (e.g., food products or pet food products) or components thereof cultured from metazoan cells, preparation methods and preparation systems.

The application relates to a Clostridium permanentii fixed with a carbon gas and application thereof in production of alcohol and cell protein, and belongs to the technical field of industrial microbial synthesis. The Clostridium permanentii is named Clostridium permanentii GG8, classified as Clostridium ljungdahlii , and preserved in the China General Microbiological Culture Collection Center. The Clostridium permanentii GG8 is obtained through adaptive laboratory evolution and sorting from a wild type strain Clostridium permanentii DSM13528. Compared with the wild type strain, the Clostridium permanentii GG8 improves the ability of producing ethanol and cell protein by taking the carbon gas as a carbon source; in a batch fermentation process, the Clostridium permanentii GG8 can accumulate cell biomass OD600 greater than or equal to 20, ethanol concentration greater than or equal to 50 g / L, and acetic acid concentration greater than or equal to 5 g / L; in a single-tank continuous fermentation process, the Clostridium permanentii GG8 can be stably fermented for more than 5 months, and the ethanol production intensity is more than 2 g / L / h, which is helpful to improving the economy of gas fermentation.

The invention discloses a method for efficiently synthesizing succinic acid by improving cell biomass and cell viability through quorum sensing regulation and control. Through overexpression of ptsH, a normal quorum sensing system of Escherichia coli can be effectively destroyed, and an AI-2 signal channel is quenched. According to the method, the autolysis rate of escherichia coli can be reduced, and the cell biomass and the cell activity can be improved. Fermentation verifies that after ptsH is over-expressed, the high thallus amount level that OD600 is larger than 7 is maintained in the whole succinic acid fermentation process, the maximum OD600 is 8.5, and the final succinic acid yield is 80.12 g / L. Compared with escherichia coli without over-expression of ptsH, the maximum OD600 is improved by 30.7%, and the yield of succinic acid is improved by 17.9%. According to the method, the cell biomass and the cell activity in the fermentation process can be effectively improved, the succinic acid production capacity of the recombinant escherichia coli is remarkably enhanced, and the method has important application value.

The invention relates to a method for improving the organoleptic properties of a vegetable protein-rich material, comprising the following steps: 1. Suspending the vegetable protein-rich material in a preferentially aqueous solvent in order to obtain a suspension having a dry matter content of between 5% and 20%; 2. Adding to the suspension of step 1 one or more microbial strains, where the one or more microbial strains are selected from the group consisting of lactic acid bacteria strains, yeast strains or mixtures thereof, so as to obtain an inoculated suspension having a cell density of between 1.105 cfu / mL and 1.1010 cfu / mL; 3. Incubating the suspension of step 2 at a temperature of between 20 DEG C and 40 DEG C for a duration of between 30 minutes and 120 minutes. The invention also relates to a vegetable protein isolate obtainable by the method according to the invention and to the use of the vegetable protein isolate.

The present invention belongs to the fields of genetic engineering and microbial technology, and specifically relates to the use of split genes in regulating threonine synthesis, cell morphology, and growth. Experiments have demonstrated that regulating the expression intensity of split genes through biological elements can affect threonine synthesis, cell morphology, and growth of target microorganisms. For example, when ftsZ expression is too strong, it has little effect on microbial cell growth and threonine production, and may even be detrimental to threonine production. However, when ftsZ expression is relatively weak, it is beneficial to the accumulation of cell biomass and promotes threonine synthesis. Therefore, by regulating the expression intensity of the split genes, dynamic regulation of threonine production by bacterial strain fermentation is achieved, which is not only beneficial for actual production but also can be used as a model strain for research on the mechanism of threonine synthesis, cell morphology, and growth, thus having excellent practical application value.