8 results about "Cell biomass" patented technology

The present invention provides a cocoa powder including at least one compound selected from the group consisting of: an essential amino acid, a sugar, a neurotransmitter, and any combination thereof, wherein the at least one compound is enriched in a biomass of Theobroma cacao cells compared to a control cocoa powder, and is derived therefrom.

The invention discloses a culture method for increasing the flavonoid content of suspension cells of codonopsis pilosula. The method comprises the following steps: (1) obtaining aseptic seedlings: disinfecting codonopsis pilosula seeds, inoculating the disinfected codonopsis pilosula seeds into an MS culture medium, and culturing to obtain the aseptic seedlings; (2) callus induction and subculture: taking the roots of the aseptic seedlings as explants, and performing induction and subculture on an MS culture medium containing 2, 4-D, 6-BA and KT to obtain loose calluses; (3) suspension culture: inoculating the callus into an MS liquid culture medium containing NAA, 6-BA and KT for suspension culture to obtain high-biomass codonopsis pilosula suspension cells; and (4) adding an elicitor / precursor: adding methyl jasmonate (MeJA) or sodium acetate (NaAC) into the suspension culture system, and inducing flavone synthesis and accumulation. By optimizing a plant growth regulator composition in the culture medium, the biomass of the suspension cells of the codonopsis pilosula is remarkably improved (the highest biomass reaches 57.57 g / L); by adding MeJA or NaAC, the daily average yield of flavone is increased to 16.69 mg / L / d, and the daily average yield of flavone is increased to 18.20 mg / L / d. According to the method, the culture period is about 70 days, and a feasible way is provided for efficient and large-scale production of codonopsis pilosula flavone.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing L-ornithine as well as a construction method and application of the recombinant corynebacterium glutamicum for producing L-ornithine, corynebacterium glutamicum SO30 is taken as an original strain, a SigD gene and a FruR gene are sequentially knocked out through a suicide plasmid pK18mobsacB mediated homologous recombination technology, and double knockout recombinant corynebacterium glutamicum SO30-SigD-FruR is constructed. The L-ornithine yield of the strain subjected to shake-flask fermentation reaches 51.88 g / L and is increased by 56.7% compared with that of an original strain SO30; the fermentation yield of a 5L bioreactor reaches 123 g / L and is increased by 57.6% compared with that of an original strain SO30, and the cell biomass and the sugar conversion rate are remarkably increased. Through global transcriptional regulation factor transformation, the existing yield bottleneck is broken through, and efficient strains and technical support are provided for industrial fermentation production of L-ornithine.

The present invention relates to a novel fermentation process using a particular strain of Kluyveromyces marxianus, referred to as KMPROTEO 1, to obtain a single-cell protein useful in the food industry.In the present description, the invention discloses a process for fermenting dextrose and other consumables to obtain a single-cell biomass. The disclosed process enables a high-protein product to be obtained that is in the range of 50 to 60% for use in the food industry. The disclosed process uses dextrose as a propagation medium for use by Kluyveromyces marxianus to obtain a single-cell biomass and, subsequently, a single-cell protein.

A three-dimensional porous growth surface (carriers) and cell culture devices including the same are provided. The carriers are made by multiple layers of netting or mesh, especially large dimension and area of fabrics that are capable to form a column-type fixed bed by rolling the layers or other shape of fixed bed by stacking or randomly disposed packing the carriers to form a packed-bed for cell culture the layers together, thereby sealing the surrounding of the multilayer nettings or meshes to reduce particle generation during cell harvest, or ease of separation by filtration due to larger wall dimension on the nettings or meshes than cells. The fixed bed make by large dimension of the growth surface can easily to manufacture a fixed bed simply by rolling the multiple layers of sheets, which can reduce the manufacture cost and also facilitate mass production of carriers for fixed bed bioreactors.

The present application belongs to the field of biotechnology, and specifically relates to a method for expressing human serum albumin by using Nicotiana sylvestris NS suspension cell line, which comprises the following steps: establishing a stably cultured Nicotiana sylvestris suspension cell system, and producing by amplification, and the obtained cell biomass can reach up to 500 g / L. Then, taking human serum albumin as the research object, transformation screening is carried out in the Nicotiana sylvestris NS suspension cell system to realize high expression of HSA. The present application removes the complex tissue regeneration step, and through plant codon optimization and subcellular targeting strategy design, aims to overcome the problem of low expression of exogenous genes in plants, so as to realize efficient enrichment of target proteins in specific organelles (such as vacuole / endoplasmic reticulum, etc.). The technical scheme of the present application can be popularized and applied to the expression and screening of other types of functional proteins, and provides important technical reference and practical support for the application of plant suspension cell lines in the field of recombinant protein expression.

This invention relates to cell biomass (e.g., food products or pet food products) or components thereof cultured from metazoan cells, preparation methods and preparation systems.

The application relates to a Clostridium permanentii fixed with a carbon gas and application thereof in production of alcohol and cell protein, and belongs to the technical field of industrial microbial synthesis. The Clostridium permanentii is named Clostridium permanentii GG8, classified as Clostridium ljungdahlii , and preserved in the China General Microbiological Culture Collection Center. The Clostridium permanentii GG8 is obtained through adaptive laboratory evolution and sorting from a wild type strain Clostridium permanentii DSM13528. Compared with the wild type strain, the Clostridium permanentii GG8 improves the ability of producing ethanol and cell protein by taking the carbon gas as a carbon source; in a batch fermentation process, the Clostridium permanentii GG8 can accumulate cell biomass OD600 greater than or equal to 20, ethanol concentration greater than or equal to 50 g / L, and acetic acid concentration greater than or equal to 5 g / L; in a single-tank continuous fermentation process, the Clostridium permanentii GG8 can be stably fermented for more than 5 months, and the ethanol production intensity is more than 2 g / L / h, which is helpful to improving the economy of gas fermentation.