Method for promoting synthesis of microalgae oil by low frequency and low intensity ultrasound

A microalgae oil, low-intensity technology, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of mutation inhomogeneity, limitation, negative impact on biomass, etc., to accelerate metabolic rate, improve The accumulation of , the effect of enhancing permeability

Active Publication Date: 2017-06-06
HARBIN INST OF TECH
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  • Application Information

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Problems solved by technology

Nutrient regulation is mainly based on the regulation of nitrogen and phosphorus elements. The lack of nitrogen, phosphorus and other elements can significantly increase the oil content of microalgae strains, but it will also have a negative impact on their biomass. Therefore, in the total oil content No obvious improvement
Increasing t

Method used

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  • Method for promoting synthesis of microalgae oil by low frequency and low intensity ultrasound
  • Method for promoting synthesis of microalgae oil by low frequency and low intensity ultrasound
  • Method for promoting synthesis of microalgae oil by low frequency and low intensity ultrasound

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Experimental program
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Embodiment 1-17

[0019] The alga species used in the present invention is Scenedesmus screened in a laboratory, and the ultrasonic experiments are all completed in a clean bench in a sterile room, and the temperature is room temperature. In order to maintain a sterile state, the ultrasonic biological growth promotion instrument and the ultrasonic probe were placed in the ultra-clean bench during the test, and sterilized by ultraviolet light for 25 minutes; Placed in a ultra-clean bench sterilized by ultraviolet light; in order to make the ultrasound evenly act on the microalgae cells, the algae liquid should be shaken before the ultrasonic treatment, and then the sterilized ultrasonic probe should be immersed in the microalgae liquid for about 2mm for ultrasonication Treatment (Due to the large diameter of the ultrasonic probe, this experiment uses a plant tissue culture bottle with a volume of about 240ml with air holes to cultivate microalgae. Note that after a sample is processed, the probe ...

Embodiment 18-27

[0026] The difference with Example 15 is in Table 2.

[0027] Table 2 Embodiment 18-27 differentiating conditions and experimental results.

[0028]

[0029]

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Abstract

The invention discloses a method for promoting the synthesis of microalgae oil by low frequency and low intensity ultrasound, aiming at the critical problem in the field of oil production from microalgae, i.e., the technical difficulty of how to improve the oil content in the microalgae. The method is characterized in that an ultrasonic probe is immersed in a microalgae culture solution which is cultured to a logarithmic phase; ultrasound is started to perform ultrasonic treatment on the microalgae, and circular treatment is carried out, wherein the ultrasonic frequency is 20-50kHz, the ultrasonic power is 10-30W/L, the ultrasonic treatment time is 15-35min/d, and the time interval of ultrasonic pulses is 1-6s; the treated microalgae are transferred to an illumination incubator for culture. The technique provides a new strategy for improving the oil content and cell biomass of the microalgae. The cultured microalgae has the advantages that not only is the synthesis amount of oil increased, but also the utilization rate of sugar and biomass are increased, which lays a good foundation for the industrialization development of the microalgae.

Description

technical field [0001] The invention relates to the field of microalgae bioenergy, and relates to a method for effectively increasing the oil content of microalgae. Background technique [0002] As the global energy shortage and environmental degradation become more and more serious, finding new clean and renewable energy has become one of the problems that need to be solved urgently. Biomass energy stands out among many new energy sources due to its advantages of safety, non-pollution, and renewability, and is expected to replace or partially replace fossil energy. As biodiesel is a research hotspot of biomass energy, the selection of its raw materials has received extensive attention. Because microalgae can be cultivated in seawater, sewage and tidal flats, they have the advantages of "not competing with people for food or land", strong photosynthesis can fix more carbon dioxide, and have high oil content. Therefore, the third generation Biodiesel has been extensively st...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12R1/89
CPCC12P7/6463
Inventor 刘冰峰裴轩瑗肖若楠马超任南琪
Owner HARBIN INST OF TECH
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