Method for promoting synthesis of microalgae oil by low frequency and low intensity ultrasound

Abstract

The invention discloses a method for promoting the synthesis of microalgae oil by low frequency and low intensity ultrasound, aiming at the critical problem in the field of oil production from microalgae, i.e., the technical difficulty of how to improve the oil content in the microalgae. The method is characterized in that an ultrasonic probe is immersed in a microalgae culture solution which is cultured to a logarithmic phase; ultrasound is started to perform ultrasonic treatment on the microalgae, and circular treatment is carried out, wherein the ultrasonic frequency is 20-50kHz, the ultrasonic power is 10-30W / L, the ultrasonic treatment time is 15-35min / d, and the time interval of ultrasonic pulses is 1-6s; the treated microalgae are transferred to an illumination incubator for culture. The technique provides a new strategy for improving the oil content and cell biomass of the microalgae. The cultured microalgae has the advantages that not only is the synthesis amount of oil increased, but also the utilization rate of sugar and biomass are increased, which lays a good foundation for the industrialization development of the microalgae.

Description

technical field [0001] The invention relates to the field of microalgae bioenergy, and relates to a method for effectively increasing the oil content of microalgae. Background technique [0002] As the global energy shortage and environmental degradation become more and more serious, finding new clean and renewable energy has become one of the problems that need to be solved urgently. Biomass energy stands out among many new energy sources due to its advantages of safety, non-pollution, and renewability, and is expected to replace or partially replace fossil energy. As biodiesel is a research hotspot of biomass energy, the selection of its raw materials has received extensive attention. Because microalgae can be cultivated in seawater, sewage and tidal flats, they have the advantages of "not competing with people for food or land", strong photosynthesis can fix more carbon dioxide, and have high oil content. Therefore, the third generation Biodiesel has been extensively st...

AI Technical Summary

Problems solved by technology

Nutrient regulation is mainly based on the regulation of nitrogen and phosphorus elements. The lack of nitrogen, phosphorus and other elements can significantly increase the oil content of microalgae strains, but it will also have a negative impact on their biomass. Therefore, in the total oil content No obvious improvement

Increasing the oil content of algae strains through mutation breeding can achieve better results, but due to the non-directional nature of its mutations, it has certain limitations

By means of genetic engineering, it is possible to increase the oil content of microalgae strains in a targeted manner, but due to its technical difficulty, it is limited.

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