Method and system for producing heterotrophic alga in high density

A density, seaweed technology, applied in the field of high-density growth of heterotrophic microalgae and production of omega-3 fatty acids, can solve problems such as by-product applications that are not discussed

Inactive Publication Date: 2010-12-01
WASHINGTON STATE UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of by-products such as crude glycerol is not discussed

Method used

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  • Method and system for producing heterotrophic alga in high density
  • Method and system for producing heterotrophic alga in high density
  • Method and system for producing heterotrophic alga in high density

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Implementation example 1. The regulation and control of the first stage reaction parameter

[0045] The effective control of the first-stage reaction parameters described in the present invention establishes fermentation conditions that convert limited cell resources into cell growth and exponential increase in cell number. See figure 2 and Table 2.

[0046] figure 2 It was shown that dissolved oxygen (DO) plays a major role in process control (in coordination with temperature and carbon / nitrogen source changes). As can be seen, as dissolved oxygen rises, cellular energy is diverted to the synthesis of chemicals necessary for cell division, thus significantly increasing cell number with little effect on cell size. Conversely, reducing dissolved oxygen will allow cellular energy to focus on cell growth and lipid accumulation.

[0047] The process control gave 181 × 10 after 24 hours at 50% DO 6 cells / ml, but decreased to 22.5×10 at 48 hours 6 cells / ml. The cell ...

Embodiment 2

[0055] The initial medium consisted of 40 g / L glycerol, 5 g / L yeast extract, 5 g / L ammonium acetate as nitrogen source and other insignificant factors in seawater. Cells from the smaller flasks were inoculated into fermentors at a 10% (v / v) inoculum size. The temperature is controlled at 30°C, and the dissolved oxygen is controlled at 20%-50%. The first stage, also called the cell number increase stage, lasts for 24 hours. During this period, the cell number increases rapidly, and the final cell density is 400-800×10 6 cells / ml, and the higher the better. When the number of cells stops increasing, the second phase begins, where fatty acids begin to accumulate. At this stage, DO is controlled at 3-5%, and the concentration of glycerol and nitrogen source is controlled at 30-50g / L and 0.5-1.0g / L respectively by feeding. The temperature was still at 30°C, and this phase lasted until the 60th-80th hour of the whole process, until the dry weight of the cells stopped increasing. ...

Embodiment 3

[0075] Implementation example 3. Energy consumption comparison

[0076] Matlab-Simulink software can compare the energy consumption of the system. Simulate seaweed fermentation in a 5-ton fermenter, and use the data of 5L and 30L experiments as the basis for evaluation.

[0077] The basic parameters set for this process are: a fermenter with an effective volume of 5 cubic meters; a fed-batch fermentation scheme; a reaction temperature of 30°C; an average room temperature of 25°C; an aeration rate of 0.5vvm and 1.0vvm, and an electricity cost of $0.046 / kwh . The basic setting parameters of the heating system are: heat loss equivalent, q is the heat loss, h is the thermal conductivity of steel, A is the internal area, Ti the real internal temperature, T is the external temperature; the required heat is equivalent, Q is The amount of heat required, M is the total mass of the solution, Cp is the heat capacity, T is the target internal temperature, and Ti is the true internal tem...

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Abstract

The invention provides a method and a system for producing heterotrophic alga in high density. In the method, unique biological characteristics of a microbial system are utilized, and the accumulation of lipid is increased by simulating cell division and the uncoupling of cell growth/amount. A process for producing polyunsaturated fatty acid (PUFA) by microalgae fermentation comprises three different stages: (1) a cell proliferation stage for increasing the number of cells; (2) a biomass and lipid accumulation stage for increasing cell biomass, particularly the amount of the lipid; and (3) a lipid concentration increasing stage by refining for producing more PUFA by adjusting the steering of the lipid. Various variables such as the types and concentration of a carbon source and nutritional factors, dissolved oxygen and temperature are changed continuously through a three-stage alga growing process to produce maximum biomass, lipid and docosahexaenoic acid (DHA) by final induction. Therefore, most cell viability leads to the cell division and the rapid formation of a large number of cells, so that the biomass and the lipid yield are integrally enhanced finally.

Description

technical field [0001] The present invention relates to methods for producing omega-3 fatty acids using microalgae. Specifically, the present invention proposes a multi-stage research and production method for the high-density growth and production of omega-3 fatty acids by heterotrophic microalgae. This approach decouples cell division, growth and production of omega-3 fatty acids by controlling their optimum conditions in their respective reactors. This uncoupling and optimization approach resulted in an overall increase in the yield and productivity of algae, lipids, and polyunsaturated fatty acids (PUFAs). Additionally, the process has been integrated into the biodiesel refining process by utilizing waste raw materials such as crude glycerol (a by-product of biofuel production) etc. as the main carbon source. Considering that the products of seaweed growth not only contain PUFA lipids but also non-polyunsaturated lipids, which can be utilized in the production of biofuel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C10L1/02C12R1/89
CPCY02E50/13Y02E50/10
Inventor 迟占有温志友克雷格·福莱尔陈树林
Owner WASHINGTON STATE UNIVERSITY
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