Method for culturing paenibacillus polymyxa at high density

A polymyx spore-like, high-density culture technology for use in microorganism-based methods, biochemical equipment and methods, bacteria, etc.

Inactive Publication Date: 2013-09-04
FUZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] At present, domestic research on Paenibacillus polymyxa mainly focuses on strain screening, isolation and identification, antibacterial effect and analysis of chemical components of fermentation broth, but there are rare reports on the fermentation and cultivation of Paenibacillus polymyxa

Method used

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  • Method for culturing paenibacillus polymyxa at high density
  • Method for culturing paenibacillus polymyxa at high density
  • Method for culturing paenibacillus polymyxa at high density

Examples

Experimental program
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Embodiment 1

[0026] 1. Determination of Growth Curve of Paenibacillus polymyxa

[0027] After activating Paenibacillus polymyxa, inoculate it into beef extract-peptone liquid medium (beef extract 3g, peptone 10g, sodium chloride 5g, distilled water 1000mL, pH7.0-7.2) at an inoculum size of 5% (v / v) In the medium, the liquid volume is 100mL / 250mL, cultured at 30°C and 200r / min, and the OD at different culture time is measured by spectrophotometry and plate colony counting method 600 value and the number of colonies, with the culture time as the abscissa, respectively with OD 600 The value and the number of colonies are plotted as a growth curve on the ordinate ( figure 1 ). According to the growth curve, the optimal culture time of Paenibacillus polymyxa was 18h.

[0028] 2. Optimization of medium formula for Paenibacillus polymyxa

[0029] The effect of different carbon sources, nitrogen sources and inorganic salts on the biomass of Paenibacillus polymyxa cells was studied using bee...

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Abstract

The invention belongs to the field of microbial fermentation and particularly relates to a method for culturing paenibacillus polymyxa at high density, which can increase the yield of paenibacillus polymyxa. According to the method, paenibacillus polymyxa serving as a strain is activated and inoculated onto a culture medium. After being optimized, the cell biomass of paenibacillus polymyxa reaches 5.3 *10<9>cfu / mL which is 15.72 times higher than before (3.17*18<8>). Through study on culture medium formula and culture conditions, the method lays a foundation for industrial fermentation and further development and utilization of paenibacillus polymyxa.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and more specifically relates to a high-density culture of Paenibacillus polymyxa ( Paenibacillus polymyxa )Methods. technical background [0002] Paenibacillus polymyxa ( Paenibacillus polymyxa ) is a spore-forming G + Bacteria, which are not pathogenic to humans, animals or plants, can produce various biologically active substances such as polypeptide antibiotics, antagonistic proteins, enzymes, plant hormones, flocculants, etc., and have the functions of biological pesticides and biological fertilizers, and have been widely used in agriculture , industrial mining and wastewater treatment and other fields. The U.S. Environmental Protection Agency (EPA) has listed it as one of the types of microorganisms that can be used commercially, and the Ministry of Agriculture of my country has also listed it as a first-class strain that is exempt from safety identification. [0003] Paenibacillus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/12
Inventor 林娟陈晓红叶秀云李仁宽严芬杨捷
Owner FUZHOU UNIV
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