Method of preparing lycopene from trispore Bruce mould

Abstract

The invention relates the method for fermenting trisporic Bang's fungus to produce lycopersicin. The carrier of oxygen is added into fermentation culture medium to provide a carrier of oxygen fermentation system, the invention improves the oxygen concentration without consuming excess energy source, and it improves cell biomass and lycopersicin content, and it improves the productivity of lycopersicin.

Description

Technical field: [0001] The invention relates to a method for preparing lycopene by fermenting the three spora boulardii. It belongs to the fields of food, medicine and chemical industry. Background technique: [0002] Lycopene is a fat-soluble carotenoid and is an intermediate in the biosynthesis of many carotenoids. Compared with β-carotene, although lycopene is not the precursor of vitamin A, its ability to eliminate singlet oxygen and free radicals and its antioxidant effect are superior to β-carotene. A series of important physiological functions such as cardiovascular disease. [0003] Lycopene is a straight-chain hydrocarbon composed of 11 conjugated and 2 non-conjugated carbon-carbon double bonds. It is easy to oxidize and isomerize, so it is difficult to extract. At present, the commonly used extraction methods are: organic solvent extraction, supercritical extraction, saponification, and its detection methods mainly include spectrophotometry, paper chromatograph...

AI Technical Summary

Problems solved by technology

[0005] In the method of using Blakeslea trispora to produce lycopene, since Blakeslea trispora is a highly aerobic and viscophilic microorganism, the medium used has a high viscosity and a high solid content, and also contains 5% vegetable oil (providing double for metabolism). key, resulting in a low dissolved oxygen rate in the fermentation system); during the growth process, it is easy to tangle into agglomerates, resulting in a far insufficient amount of dissolved oxygen in the logarithmic growth period, so the problem of oxygen supply is to restrict the biological reaction in the fermentation process of B. trispora and important factors of life process (Jeong JC.Lee IY.Kim SW.Park YH.1999.Biotechnol.Lett.21:683-686; Kim SW.SeoWT.Park YH.1997.J.Ferment.Bioeng.84:330 -332)

[0006] For most fermentations, the lack of oxygen will cause metabolic abnormalities, biomass and target metabolite yield reduction; and because the solubility of oxygen in aqueous solution is very low (its saturation is less than 7mg / L), the oxygen supply in fermentation is very low It is easy to become the main contradiction, so how to ensure the supply of oxygen in fermentation to meet the oxygen demand of production strains is one of the keys to improve fermentation production capacity and reduce costs

At present, the traditional mechanical oxygen supply methods such as stirring ventilation and shaking table oscillation have the following problems: when the operating conditions of the equipment are reduced, the oxygen supply is severely limited, and the formation of target metabolites is blocked; when the operating conditions are increased, the high mechanical strength will be enhanced The damage to the cells caused by shearing will cause the bacteria to be damaged and cannot metabolize normally, and the operating cost will also increase greatly; while improving the design of bioreactors, stirring paddles and air distributors, and using oxygen-enriched air will reduce production costs. greatly improved, so it is necessary to find a new way to strengthen oxygen supply