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Method for effectively suppressing initial-stage browning phenomenon of liquorice cell suspension culture

A technology of seed cells and cells, which is applied in biochemical equipment and methods, plant cells, tissue culture, etc., can solve the problems of ineffective inhibition of browning, shortening of subculture time, time-consuming and labor-intensive effects, and achieves a good effect of inhibiting browning , good growth status, and increased cell biomass accumulation

Inactive Publication Date: 2014-02-19
INNER MONGOLIA UNIV OF SCI & TECH
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AI Technical Summary

Problems solved by technology

However, these treatments are often not effective in inhibiting browning
Shorten subculture time, continuous transfer of explants is not only time-consuming and labor-intensive but also greatly increases the chance of contamination

Method used

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  • Method for effectively suppressing initial-stage browning phenomenon of liquorice cell suspension culture
  • Method for effectively suppressing initial-stage browning phenomenon of liquorice cell suspension culture
  • Method for effectively suppressing initial-stage browning phenomenon of liquorice cell suspension culture

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Embodiment Construction

[0020] The implementation of the present invention is described in further detail below:

[0021] A method for inhibiting the browning of licorice cells after solid-liquid conversion, which adopts the following steps:

[0022] Step 1: using licorice cells in good growth state through continuous subculture on solid medium as seed cells for suspension culture;

[0023] The solid medium is a modified MS (Murashige-Skoog) medium, that is, based on the basic MS medium, it contains the following substances in mass volume concentration: 0.5 mg of 2,4-dichlorophenoxyacetic acid (2,4-D) / L, 6-benzylaminoadenine (6-BA) 0.5mg / L, naphthaleneacetic acid (NAA) 0.5mg / L, sucrose 30g / L, agar 8-10g / L, adjust the pH to 5.8- 6.0.

[0024] The basic MS medium components include macroelements: 1.65g / LNH 4 NO 3 , 1.90g / L KNO 3 , 0.17g / L KH 2 PO 4 , 0.1807g / L MgSO 4 ·7H 2 O, 0.332g / L CaCl 2 ;Trace elements: 22.3mg / L MgSO 4 4H 2 O, 6.2 mg / L N 3 BO 3 , 8.6mg / L ZnSO 4 ·7H 2 O, 0.83mg / L K...

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Abstract

The invention provides a method for effectively suppressing a browning phenomenon after liquorice cell solid-liquid transformation with simple operation, and belongs to the field of plant cell culture. The method comprises the steps of selecting and inoculating seed cells, culturing by controlling rotating speed in different stages, rinsing the cells and carrying out suspension culture. The browning phenomenon after the liquorice cell solid-liquid transformation process is obviously suppressed; cell biomass accumulation is obviously increased; compared with a control group (without addition of an inhibitor, rotating speed control at different stages and rinsing process), the relative browning degree of the suspension system is reduced by 55.8% maximally, and the biomass (wet weight) accumulation is at most increased by 35.2%. The obtained liquorice suspension cells are uniformly dispersed, well in growth situation, and can be used as the excellent seed cells for further propagation or an excellent scientific research material.

Description

Technical field: [0001] The invention mainly relates to a method for effectively inhibiting the initial browning phenomenon of licorice cell suspension culture, which belongs to the field of plant cell culture. Background technique: [0002] Licorice (Glyrrhiza uralensis Fisch), a perennial herb or subshrub of the leguminous family, is one of the oldest and most widely used Chinese herbal medicines in the world. In recent years, with the sharp increase in clinical application of licorice and the expansion of the scope of development and utilization of licorice, the demand and export of licorice have increased sharply. A drastic reduction in resources. In order to solve the contradiction between supply and demand of licorice, artificial licorice planting has become the first choice. But the disease of cultivated licorice is serious; both the output and the quality are far from meeting the existing demand, and it is imminent to solve the problem of licorice resources. [00...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/02
Inventor 李雅丽孟婷婷高山王秀娟
Owner INNER MONGOLIA UNIV OF SCI & TECH
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