38results about How to "Increased biomass accumulation" patented technology

The invention discloses an improved culture medium suitable for vegetative growth of nostoc sphaeroids kutz and a preparation method. The preparation method comprises the following steps: respectively preparing a solution A mother solution, a solution B mother solution, a solution C mother solution, a solution D mother solution, a solution E mother solution and a solution F mother solution;sequentially dissolving a certain amount of mother liquor of the solution A, the solution B, the solution C, the solution D, the solution E and the solution F into a solvent during use, adjusting the pH value to 7.1 by using sodium hydroxide or hydrochloric acid, and sterilizing for 20 minutes at 121 DEG C and 0.1 Mpa. The solution A is prepared from 25.0 g / L of sodium nitrate, 0.800 g / L of dipotassium phosphate and 1.50 g / L of magnesium sulfate heptahydrate; the solution B is prepared from 3.00 g / L of citric acid, 3.00 g / L of ferric ammonium citrate and 0.500 g / L of ethylenediamine tetraacetic acid disodium salt; the solution C is 13.6 g / L anhydrous calcium chloride; the solution D is 20.0 g / L sodium carbonate; the solution E is prepared from 2.86 g / L of boric acid, 1.81 g / L of manganese chloride tetrahydrate, 0.220 g / L of zinc sulfate heptahydrate, 0.390 g / L of sodium molybdate, 0.0800 g / L of copper sulfate pentahydrate and 0.0400 g / L of cobalt chloride hexahydrate; the solution F is a soil leaching solution preparedby adding 1000mL of clean tap water into 1000g of soil, stirring, and filtering with filter paper; and the solvent is distilled water. The improved culture medium can be stored.

The invention discloses a method for promoting the growth of plants under low-nitrogen conditions, which comprises adopting the general gene engineering method to inactivate AtLNT1 genes or disenableAtLNT1 genes to be expressed. With the method, the number of the branch roots of plants can be increased under low-nitrogen conditions, and the capability of absorbing nitrogen nutritive elements of plants is improved; the nitrogen white content of plants can be improved; and the photosynthesis of plants is improved. On the whole, the method disclosed by the invention is remarkably effective for promoting the growth of plants under low-nitrogen conditions.

The invention discloses a screening method for optimal salt and nitrogen conditions of reed seedlings, which belongs to the technical field of ecological restoration, and specifically relates to a screening method for optimal salt and nitrogen conditions of reed seedlings in saline-alkali wetlands. The purpose of the invention is to solve the problems of low survival rate of transplanted reeds and slow growth of seedlings in the existing degraded reed wetlands. A screening method for optimal salt and nitrogen conditions for reed seedlings: 1. Collection of reed rhizomes; In the case of nitrogen concentration / nitrogen addition concentration combination, the seedlings were cultivated respectively; 4. Detection and analysis: according to the plant height of reed seedlings, the biomass of aboveground plants of reed seedlings, the biomass of underground rhizomes of reed seedlings, and the total biomass of reed seedlings, SPSS 19.0 was used One-way analysis of variance was carried out, and finally the optimal salt and nitrogen conditions for reed seedlings were obtained. Advantages: The survival rate of transplanted reed seedlings reaches 100%. The invention is mainly used for rapid recovery and reconstruction of reeds in saline-alkali wetlands.

The invention discloses an improved culture medium applicable to Haematococcus pluvialis vegetative growth and a preparation method of the improved culture medium. The preparation method of the improved culture medium comprises the following steps: firstly, respectively preparing mother liquor of a solution A, mother liquor of a solution B, mother liquor of a solution C and mother liquor of a solution D, wherein the solution A comprises potassium nitrate with the concentration of 200 mg / L, dipotassium phosphate with the concentration of 20 mg / L and magnesium sulfate heptahydrate with the concentration of 100 mg / L; the solution B is anhydrous calcium chloride with the concentration of 40.5 mg / L; the solution C comprises ethylenediamine tetraacetic acid disodium salt with the concentration of 19.8 [mu]g / L and ferric trichloride hexahydrate with the concentration of 24.4 [mu]g / L; the solution D comprises zinc chloride with the concentration of 4.1 [mu]g / L, boric acid with the concentration of 61 [mu]g / L, cobalt chloride hexahydrate with the concentration of 5.1 [mu]g / L, copper sulfate pentahydrate with the concentration of 6.0 [mu]g / L, manganese chloride hexahydrate with the concentration of 4.1 [mu]g / L, and ammonium molybdate tetrahydrate with the concentration of 38 [mu]g / L; sterilized tap water serves as a solvent; secondly, during use, dissolving the fixed amount of the solution A, solution B, solution C and solution D into the solvent in sequence, adjusting the pH value to be 6.9-7.1, and inoculating the culture medium with strains of the Haematococcus pluvialis. The improved MCM culture medium provided by the invention is balanced in nutrition, low in cost, simple to operate during preparation, and high in growth rate and biomass accumulation of cultivated strains, can be stored, and facilitates the improvement of industrialized production efficiency.

The invention discloses a long-term method for inhibiting the browning phenomenon in the suspension cell culture process of Glycyrrhizae glabra. In order to be able to suppress the browning phenomenon in the suspension cell culture process of Glycyrrhiza glabra over a long period of time, the present invention selects ascorbic acid derivatives (calcium ascorbate) instead of ascorbic acid as the anti-browning agent, and simultaneously screens out lactose instead of sucrose as a carbon source for glazing. Glycyrrhiza glabra suspension cells were cultured, and it was found that this culture method can inhibit the activity of polyphenol oxidase in suspension cells for a long time (20 days), thereby inhibiting the occurrence of browning of Glycyrrhiza glabra suspension cells, and at the same time, the biomass accumulation of suspension cells obviously increase. In addition, the method of the invention is simple to operate, and the obtained licorice glabra suspension cells have good dispersibility and good growth state. The proposal of the invention provides an effective technical means for the enlarged culture of Glycyrrhiza glabra cells.

The invention provides a method for expressing Vitiligo hyaline hemoglobin in Phaeodactylum tricornutum for autotrophic and adjunct culture, mainly by optimizing the VHb gene codon and constructing the expression vector to construct the VHb expression vector, and the expression vector Use PCR to amplify the linearized fragment, introduce it into cells by electroporation, and obtain transgenic algal strains by screening; the transgenic algal strains obtained in the present invention can continuously improve the effect of the gene on Phaeodactylum tricornutum under autotrophic and concurrent conditions. Growth efficiency and production of high value-added products.

The present disclosure provides a novel modified gene, rGRF3, or an ortholog thereof, which is shown to be decoupled from control by miR396, particularly in the presence of over-expression of at least one GIF gene, such as GIF1, AtGIF 2, AtGIF 3, Os11g40100, Os12g31350, Os03g52320 or combinations thereof. When present in a plant, the rGRF3 results in a phenotype of increased productivity (e.g. increased yield, increased biomass, increased stress resistance, increased seed production, increased seed yield, increased root growth, increased root elongation speed, delayed leaf senescence or increased drought tolerance and combinations thereof).