38results about How to "Increased biomass accumulation" patented technology

The invention belongs to the technical field of aquatic product processing or biophysical processing, and relates to a method for improving tribonema biomass and metabolites by using magnetic field intervention. The specific steps are as follows: firstly, preparing a BG11 culture medium, pouring the culture medium into a column reactor, and inoculating the culture medium with tribonema sp. in logarithmic phase; placing the reactor in a climate incubator, setting a certain temperature, illumination intensity and illumination time, introducing a certain amount of air containing CO2, and carryingout culturing; and after 1 day of culturing, applying additional magnetic field treatment, continuing the culturing, and detecting biomass and oil accumulation after 7 days of culturing. The method can increase the content of lipids, saccharides, proteins or natural metabolites with natural biological activity in the cells by adding a magnetic field to a culture system of filamentous microalgae such as the tribonema, and can fully exert the economic potential of the filamentous microalgae.

The invention relates to a method for optimizing the water level height of a Sanjiang currant seedling and a method for rapidly recovering degraded saline-alkali wetland plants, belonging to the technical field of ecological restoration and aiming at determining the influence of the water level height on the growth of the three-river currant seedling. The method for optimizing the water level height comprises the following steps of: 1, collecting the corms of the field three-river currant; secondly, indoor seedling raising; 3, preparing a substrate; fourthly, setting a plurality of water levelgradients, and respectively culturing the Sanjiang currant seedlings; 5, calculating the relative growth rate of the seedlings, and measuring the biomass of the currant; and 6, measuring and analyzing. The quick recovery method comprises the following steps of: injecting water into a recovery area until the water depth is between 40cm and 50cm when the Sanjiang currant bulb grows out and the height of the Sanjiang currant seedlings is between 12 and 18cm. According to the method for optimizing water level height of Sanjiang currant seedling and method for rapidly recovering degraded saline-alkali wetland plants , the optimal water level height of the growth of the Sanjiang currant seedlings is determined by researching the influence of the water level on the growth of the three-river currant seedlings, so that the accumulation of the biomass of the Sanjiang currant is effectively improved, and the rapid planting and construction of the Sanjiang currant population are facilitated.

The invention discloses a method for inhibiting browning in glycyrrhiza glabra suspension cell culture process for long time, and aims at inhibiting browning in the glycyrrhiza glabra suspension cellculture process for long time. The method is characterized in that an ascorbic acid derivate (calcium ascorbate) is adopted to replace ascorbic acid as a browning resisting agent, and meanwhile, lactose is screened to replace saccharose as a carbon source in the culture of glycyrrhiza glabra suspension cells. The result shows that the activity of polyphenol oxidase in the suspension cells can be inhibited for long time (20d) by the culture method, and the browning in the glycyrrhiza glabra suspension cell culture process can be inhibited; and meanwhile, the biomass accumulation of the suspension cells is obviously increased. Additionally, the method is simple to operate; the obtained glycyrrhiza glabra suspension cells are high in dispersing performance and good in growing state. An effective technical mean is provided for amplification culture of glycyrrhiza glabra cells.

The invention discloses a seed dressing agent for promoting premature germination of peanuts and a preparation method thereof, belonging to the technical field of biological agents. The seed dressing agent for promoting premature germination of peanuts disclosed by the invention comprises at least one selected from melatonin and gibberellin, and further comprises a bactericide and an adhesive. When the seed dressing agent is used for treating peanut seeds, growth of peanut root systems can be promoted, nutrient absorption of plants is improved, biomass accumulation is increased, and finally, peanut yield is increased.

The present invention relates generally to the use of plants as bioreactors for the production of molecules having useful properties such as inter alia polymers, metabolites, proteins, pharmaceuticals and nutraceuticals. More particularly, the present invention contemplates the use of grasses, and even more particularly C4 grasses, such as sugar-cane, for the production of a range of compounds such as, for example, polyhydroxyalkanoates, pHBA, vanillin, indigo, adipic acid, 2-phenylethanol, 1,3-propane-diol, sorbitol, fructan polymers and lactic acid as well as other products including, inter alia, other plastics, silks, carbohydrates, therapeutic and nutraceutic proteins and antibodies. The present invention further extends to transgenic plants and, in particular, transgenic C4 grass plants, capable of producing the compounds noted above and other products, and to methods for generating such plants. The ability to utilize the high growth rate and efficient carbon fixation of C4 grasses is advantageous, in that it obviates the significant growth penalties observed in other plants, and results in high yields of desired product without necessarily causing concomitant deleterious effects on individual plants. In addition, the C4 grass, sugarcane, is particularly advantageous, as in addition to the features common to all C4 grasses, this plant accumulates sucrose. This sucrose store provides a ready supply of carbon based compounds and energy which may further obviate any deleterious effects on the growth of the plant associated with the production of the product. The present invention provides, therefore, a bioreactor system comprising a genetically modified plant designed to produce particular metabolic or biosynthetic products of interest.

The invention discloses a method for promoting the growth of plants under low-nitrogen conditions, which comprises adopting the general gene engineering method to inactivate AtLNT1 genes or disenable AtLNT1 genes to be expressed. With the method, the number of the branch roots of plants can be increased under low-nitrogen conditions, and the capability of absorbing nitrogen nutritive elements of plants is improved; the nitrogen white content of plants can be improved; and the photosynthesis of plants is improved. On the whole, the method disclosed by the invention is remarkably effective for promoting the growth of plants under low-nitrogen conditions.

The invention discloses a method for preparing whole cells of gluconobacter oxydans by using glycerol as a carbon source. The method includes performing genetic engineer transformation on wild-type gluconobacter oxydans to make the wild-type gluconobacter oxydans grow with the glycerol as the carbon source, to make a recombinant gluconobacter oxydans DeltaGOX1068DeltaGOX0854, and then inoculatingthe obtained recombinant gluconobacter oxydans in a glycerol inorganic salt medium, to obtain the whole cells of gluconobacter oxydans after shaking culture through a shaking table at 30+ / -1 DEG C. Experiments prove that the process of preparing the whole cells in the method is simple and convenient, a prepared whole cell biocatalyst has catalytic efficiency equivalent to that of a gluconobacter oxydans 621H whole cell biocatalyst prepared in a sorbitol complex medium, and the biocatalyst has a low preparation cost and has good application values.

The invention discloses an improved culture medium suitable for vegetative growth of nostoc sphaeroids kutz and a preparation method. The preparation method comprises the following steps: respectively preparing a solution A mother solution, a solution B mother solution, a solution C mother solution, a solution D mother solution, a solution E mother solution and a solution F mother solution;sequentially dissolving a certain amount of mother liquor of the solution A, the solution B, the solution C, the solution D, the solution E and the solution F into a solvent during use, adjusting the pH value to 7.1 by using sodium hydroxide or hydrochloric acid, and sterilizing for 20 minutes at 121 DEG C and 0.1 Mpa. The solution A is prepared from 25.0 g / L of sodium nitrate, 0.800 g / L of dipotassium phosphate and 1.50 g / L of magnesium sulfate heptahydrate; the solution B is prepared from 3.00 g / L of citric acid, 3.00 g / L of ferric ammonium citrate and 0.500 g / L of ethylenediamine tetraacetic acid disodium salt; the solution C is 13.6 g / L anhydrous calcium chloride; the solution D is 20.0 g / L sodium carbonate; the solution E is prepared from 2.86 g / L of boric acid, 1.81 g / L of manganese chloride tetrahydrate, 0.220 g / L of zinc sulfate heptahydrate, 0.390 g / L of sodium molybdate, 0.0800 g / L of copper sulfate pentahydrate and 0.0400 g / L of cobalt chloride hexahydrate; the solution F is a soil leaching solution preparedby adding 1000mL of clean tap water into 1000g of soil, stirring, and filtering with filter paper; and the solvent is distilled water. The improved culture medium can be stored.

The invention discloses a method for promoting the growth of plants under low-nitrogen conditions, which comprises adopting the general gene engineering method to inactivate AtLNT1 genes or disenableAtLNT1 genes to be expressed. With the method, the number of the branch roots of plants can be increased under low-nitrogen conditions, and the capability of absorbing nitrogen nutritive elements of plants is improved; the nitrogen white content of plants can be improved; and the photosynthesis of plants is improved. On the whole, the method disclosed by the invention is remarkably effective for promoting the growth of plants under low-nitrogen conditions.

The invention discloses an endophytic fungus of the genus Phoma and its application. The Phoma sp. endophytic fungus is named as Phoma sp. KDZ-5, and the preservation number is CCTCC No.M 2014162. The application is the application of the endophytic fungus of the Phoma genus in improving the salt tolerance of rice or wheat. The present invention finds for the first time that Phoma sp. KDZ-5 can improve the resistance ability of rice and wheat to salt stress. The test shows that after establishing a co-cultivation system with KDZ-5 strain and rice seedlings, the KDZ-5 strain can significantly Promote the growth of rice seedlings on salt-added MS plates; after establishing a co-culture system with wheat seedlings under nutrient solution hydroponic and soil pot conditions, KDZ-5 strains can significantly slow down or delay the salinity of wheat in saline-alkali soil damage symptoms, while greatly increasing the biomass accumulation of wheat.

The invention discloses a screening method for optimal salt and nitrogen conditions of reed seedlings, which belongs to the technical field of ecological restoration, and specifically relates to a screening method for optimal salt and nitrogen conditions of reed seedlings in saline-alkali wetlands. The purpose of the invention is to solve the problems of low survival rate of transplanted reeds and slow growth of seedlings in the existing degraded reed wetlands. A screening method for optimal salt and nitrogen conditions for reed seedlings: 1. Collection of reed rhizomes; In the case of nitrogen concentration / nitrogen addition concentration combination, the seedlings were cultivated respectively; 4. Detection and analysis: according to the plant height of reed seedlings, the biomass of aboveground plants of reed seedlings, the biomass of underground rhizomes of reed seedlings, and the total biomass of reed seedlings, SPSS 19.0 was used One-way analysis of variance was carried out, and finally the optimal salt and nitrogen conditions for reed seedlings were obtained. Advantages: The survival rate of transplanted reed seedlings reaches 100%. The invention is mainly used for rapid recovery and reconstruction of reeds in saline-alkali wetlands.

The invention provides a compound fertilizer for cordate houttuynia and a preparation method thereof, the compound fertilizer comprises the following components in parts by weight: 30-36 parts of urea, 40-48 parts of potassium sulfate, 10-16 parts of industrial monoammonium and 0.6-1 part of trace elements, and also comprises 6-10 mg / Kg of polyurethane and 0.08-0.12 mg / Kg of intelligent hearing. The fertilizer also comprises laxogenin, and the content of the laxogenin in the fertilizer is 0.2-0.6 mg / kg; and the content of the potassium brownish yellow rot in the fertilizer is 6-10mg / kg. The compound fertilizer provided by the invention contains a large number of nutrient elements and trace elements required by the growth of cordate houttuynia crops, and polyurethane peptide, smart hearing aid, potassium brownish fulvic acid and laxogenin are added as required, so that the compound fertilizer is relatively comprehensive in nutrients, can better promote the growth and development of the cordate houttuynia crops, and can improve the yield of the cordate houttuynia crops.

The invention discloses an improved culture medium applicable to Haematococcus pluvialis vegetative growth and a preparation method of the improved culture medium. The preparation method of the improved culture medium comprises the following steps: firstly, respectively preparing mother liquor of a solution A, mother liquor of a solution B, mother liquor of a solution C and mother liquor of a solution D, wherein the solution A comprises potassium nitrate with the concentration of 200 mg / L, dipotassium phosphate with the concentration of 20 mg / L and magnesium sulfate heptahydrate with the concentration of 100 mg / L; the solution B is anhydrous calcium chloride with the concentration of 40.5 mg / L; the solution C comprises ethylenediamine tetraacetic acid disodium salt with the concentration of 19.8 [mu]g / L and ferric trichloride hexahydrate with the concentration of 24.4 [mu]g / L; the solution D comprises zinc chloride with the concentration of 4.1 [mu]g / L, boric acid with the concentration of 61 [mu]g / L, cobalt chloride hexahydrate with the concentration of 5.1 [mu]g / L, copper sulfate pentahydrate with the concentration of 6.0 [mu]g / L, manganese chloride hexahydrate with the concentration of 4.1 [mu]g / L, and ammonium molybdate tetrahydrate with the concentration of 38 [mu]g / L; sterilized tap water serves as a solvent; secondly, during use, dissolving the fixed amount of the solution A, solution B, solution C and solution D into the solvent in sequence, adjusting the pH value to be 6.9-7.1, and inoculating the culture medium with strains of the Haematococcus pluvialis. The improved MCM culture medium provided by the invention is balanced in nutrition, low in cost, simple to operate during preparation, and high in growth rate and biomass accumulation of cultivated strains, can be stored, and facilitates the improvement of industrialized production efficiency.

The invention discloses a long-term method for inhibiting the browning phenomenon in the suspension cell culture process of Glycyrrhizae glabra. In order to be able to suppress the browning phenomenon in the suspension cell culture process of Glycyrrhiza glabra over a long period of time, the present invention selects ascorbic acid derivatives (calcium ascorbate) instead of ascorbic acid as the anti-browning agent, and simultaneously screens out lactose instead of sucrose as a carbon source for glazing. Glycyrrhiza glabra suspension cells were cultured, and it was found that this culture method can inhibit the activity of polyphenol oxidase in suspension cells for a long time (20 days), thereby inhibiting the occurrence of browning of Glycyrrhiza glabra suspension cells, and at the same time, the biomass accumulation of suspension cells obviously increase. In addition, the method of the invention is simple to operate, and the obtained licorice glabra suspension cells have good dispersibility and good growth state. The proposal of the invention provides an effective technical means for the enlarged culture of Glycyrrhiza glabra cells.

The invention discloses a method for improving the carbon fixation efficiency of microalgae. Chlamydomonas chloroplast-type glyceraldehyde-3-phosphate dehydrogenase gene was overexpressed to enhance the ability of Chlamydomonas photosynthetic carbon fixation. It specifically includes the construction of a glyceraldehyde-3-phosphate dehydrogenase gene recombinant expression vector, and transforming the glyceraldehyde-3-phosphate dehydrogenase gene recombinant expression vector into Chlamydomonas reinhardtii cells by electric shock transformation, and screening to obtain glyceraldehyde-3 ‑Phosphate dehydrogenase enhanced expression in transgenic Chlamydomonas reinhardtii. Microalgae can synthesize biomass such as oil, protein, starch, and carotenoids through photosynthetic carbon fixation, and accelerating the production efficiency of microalgal biomass plays an important role in downstream industries. The invention transfers the target gene into Chlamydomonas reinhardtii, accelerates the carbon fixation efficiency and increases its growth rate, and has significant application prospects in the field of microalgae bioengineering.

The invention provides a method for expressing Vitiligo hyaline hemoglobin in Phaeodactylum tricornutum for autotrophic and adjunct culture, mainly by optimizing the VHb gene codon and constructing the expression vector to construct the VHb expression vector, and the expression vector Use PCR to amplify the linearized fragment, introduce it into cells by electroporation, and obtain transgenic algal strains by screening; the transgenic algal strains obtained in the present invention can continuously improve the effect of the gene on Phaeodactylum tricornutum under autotrophic and concurrent conditions. Growth efficiency and production of high value-added products.

The present disclosure provides a novel modified gene, rGRF3, or an ortholog thereof, which is shown to be decoupled from control by miR396, particularly in the presence of over-expression of at least one GIF gene, such as GIF1, AtGIF 2, AtGIF 3, Os11g40100, Os12g31350, Os03g52320 or combinations thereof. When present in a plant, the rGRF3 results in a phenotype of increased productivity (e.g. increased yield, increased biomass, increased stress resistance, increased seed production, increased seed yield, increased root growth, increased root elongation speed, delayed leaf senescence or increased drought tolerance and combinations thereof).