A long-term method for inhibiting the browning phenomenon of Glycyrrhizae glabra suspension cell culture

Abstract

The invention discloses a long-term method for inhibiting the browning phenomenon in the suspension cell culture process of Glycyrrhizae glabra. In order to be able to suppress the browning phenomenon in the suspension cell culture process of Glycyrrhiza glabra over a long period of time, the present invention selects ascorbic acid derivatives (calcium ascorbate) instead of ascorbic acid as the anti-browning agent, and simultaneously screens out lactose instead of sucrose as a carbon source for glazing. Glycyrrhiza glabra suspension cells were cultured, and it was found that this culture method can inhibit the activity of polyphenol oxidase in suspension cells for a long time (20 days), thereby inhibiting the occurrence of browning of Glycyrrhiza glabra suspension cells, and at the same time, the biomass accumulation of suspension cells obviously increase. In addition, the method of the invention is simple to operate, and the obtained licorice glabra suspension cells have good dispersibility and good growth state. The proposal of the invention provides an effective technical means for the enlarged culture of Glycyrrhiza glabra cells.

Description

technical field [0001] The invention relates to a method for culturing suspension cells of Glycyrrhizae glabra, in particular to a method capable of suppressing the browning phenomenon in the cultivation process of suspension cells of Glycyrrhiza glabra for a long time, and cultivating the suspension cells of Glycyrrhiza glabra. The invention belongs to the technical field of cell culture. Background technique [0002] Licorice (Glyrrhlza uralensis Fisch) belongs to the leguminosae plant and Papilionaceae subfamily, and is mainly distributed in the northeast, north and northwest of China. In China, there are Glycyrrhiza uralensis Fisch., Glycyrrhiza inflate Bat., Glycyrrhiza glabra L., Glycyrrhiza aspera Pall., Glycyrrhiza squamulosa Franch. , Glycyrrhiza inflata Batal., Glycyrrhiza pallidiflora Maxim., Glycyrrhiza yunnanensis Chengf. and Glycyrrhiza eglandulosa X.Y.Li. There are more than 400 known compounds in licorice, among which triterpenoid saponins and flavonoids ar...

AI Technical Summary

Problems solved by technology

These methods can effectively suppress the browning phenomenon of licorice suspension cells in short-term cultivation (within 10 days), but cannot effectively inhibit the browning phenomenon of licorice suspension cells in long-term cultivation (12-20 days).

Replacing the medium and supplementing the anti-browning agent during the cultivation process will increase the pollution rate of the operation and increase the workload, which is not conducive to the scale-up cultivation

When the adsorbent is added to the culture medium, it will adsorb the hormone in the culture system, resulting in a decrease in the concentration of the hormone in the medium. At the same time, the anti-browning effect of the adsorbent is not significant, and the accumulation of biomass (wet weight of cells) does not change significantly.

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