Method for accelerating vegetation in low-nitrogen condition

A technology of growth method and conditions, applied in the field of plant genetic engineering, can solve problems such as no, and achieve the effects of reducing the degree of damage, enhancing photosynthesis, and strengthening the ability to resist adversity and stress.

Inactive Publication Date: 2008-09-24
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the MYB superfamily has a variety of functions, so far there is no report that it can function under low nitrogen stress

Method used

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  • Method for accelerating vegetation in low-nitrogen condition
  • Method for accelerating vegetation in low-nitrogen condition
  • Method for accelerating vegetation in low-nitrogen condition

Examples

Experimental program
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Embodiment 1

[0028]In this experiment, Arabidopsis wild-type WT (Columbia ecotype), T-DNA insertion mutants lnt1-1 and lnt1-2, and mutant complementation materials AtLNT1+lnt1, promoterAtLNT1::GUS transgenic lines were used. The Arabidopsis mutants lnt1-1 (SALK_074896) and lnt1-2 (SALK_051843) were purchased from Salk Institute Genomic Analysis Laboratory (http: / / signal.salk.edu / ).

[0029] 1. The preparation method of mutant complementation material AtLNT1+lnt1, promoterAtLNT1::GUS transgenic line is as follows:

[0030] 1. AtLNT1 RNA isolation and cDNA reverse transcription:

[0031] Isolation of total RNA and reverse transcription of cDNA can be carried out according to conventional methods ("Molecular Cloning Experiment Guide" (Third Edition) J. Sambrook, Science Press).

[0032] 2. Preparation of mutant complementary material AtLNT1+lnt1:

[0033] AtLNT1 cDNA was amplified with primers 5'-AAGCTT CTATGCAGGAACGTTG-3' and 5'-GTCGACCCAAAAGGATATGATAAT-3', after digestion with Hind III an...

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Abstract

The invention discloses a method for promoting the growth of plants under low-nitrogen conditions, which comprises adopting the general gene engineering method to inactivate AtLNT1 genes or disenable AtLNT1 genes to be expressed. With the method, the number of the branch roots of plants can be increased under low-nitrogen conditions, and the capability of absorbing nitrogen nutritive elements of plants is improved; the nitrogen white content of plants can be improved; and the photosynthesis of plants is improved. On the whole, the method disclosed by the invention is remarkably effective for promoting the growth of plants under low-nitrogen conditions.

Description

technical field [0001] The invention relates to a method for promoting plant growth, in particular to a method for promoting plant growth under low-nitrogen conditions by means of genetic engineering, and belongs to the field of plant genetic engineering. Background technique [0002] Nitrogen is an essential macroelement for plant growth and development, and is the main component of many important organic substances in plants. Although nitrogen exists in various forms in the ecosphere, plants generally can only use the readily available nitrogen in the soil, including inorganic nitrogen (nitrate nitrogen and ammonium nitrogen) and organic nitrogen (amino acids). The available nitrogen content in the soil is very low, and the total nitrogen content of most cultivated land is below 0.2%, or even below 0.075%. Therefore, the growth and development of plants are often severely deficient in nitrogen supply, and nitrogen stress has already become the biggest factor limiting plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/09C12N15/82C12N15/29
Inventor 王道文谢钰容安学丽秦焕菊刘昕
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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