An improved medium suitable for the vegetative growth of Haematococcus pluvialis and its preparation method

A technology of Haematococcus pluvialis and a preparation method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high raw material prices, lack of nutrients, and inability to store, and achieves low cost and balanced nutrition. , the effect of improving production efficiency

Inactive Publication Date: 2017-11-10
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention aims at the deficiencies in the prior art, and provides a kind of improved culture medium and preparation method suitable for the vegetative growth of Haematococcus pluvialis, improves the traditional MCM culture medium, can effectively solve the traditional The culture medium is easy to generate precipitation, leading to nutrient deficiency, unable to store, cumbersome to use, high raw material price and other technical defects

Method used

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  • An improved medium suitable for the vegetative growth of Haematococcus pluvialis and its preparation method
  • An improved medium suitable for the vegetative growth of Haematococcus pluvialis and its preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for preparing an improved medium suitable for the vegetative growth of Haematococcus pluvialis, comprising the following steps:

[0023] (1) Prepare the mother liquor of solution A: Weigh 20g of potassium nitrate and dissolve it in a small amount of solvent to completely dissolve it; weigh 2g of dipotassium hydrogen phosphate and dissolve it in a small amount of solvent to completely dissolve it; weigh 10g of magnesium sulfate heptahydrate and dissolve it in a small amount of solvent Make it completely dissolved in the solvent; mix the dissolved potassium nitrate solution, dipotassium hydrogen phosphate solution and magnesium sulfate heptahydrate solution evenly, and use the solvent to make the volume to 1L to make A liquid mother liquor, so that the concentration of potassium nitrate is 20g / L, phosphoric acid The concentration of dipotassium hydrogen is 2g / L, and the concentration of magnesium sulfate heptahydrate is 10g / L;

[0024] (2) Preparation of solution...

Embodiment 2

[0032] Embodiment 2: The present invention is now equipped with improved medium to cultivate 712 strains of Haematococcus pluvialis:

[0033] (1) Preparation: 6 5L transparent glass conical flasks that have been autoclaved, 3 5L transparent glass conical flasks of the experimental group contain the improved MCM medium of the present invention, and 3 5L transparent glass conical flasks of the control group contain traditional MCM medium, the pH is adjusted to 7 with a pH meter, and the medium is ready-to-use, and each bottle contains 3L of medium;

[0034] (2) Inoculation: On the 1st day, 712 strains of Haematococcus pluvialis in the exponential growth phase were inoculated respectively in the experimental group and the control group, so that the cell inoculation density in each Erlenmeyer flask was 1.0×10 6 cells / ml;

[0035] (3) Culture: the experimental group and the control group were cultured in the same light incubator, the light-dark cycle was 12:12, the temperature was...

Embodiment 3

[0037] Embodiment 3: The improved culture medium of the present invention stored for 1 month cultivated 712 strains of Haematococcus pluvialis:

[0038] (1) Preparation: 6 5L transparent glass conical flasks that have been autoclaved, 3 5L transparent glass conical flasks of the experimental group contain the improved MCM medium of the present invention, and 3 5L transparent glass conical flasks of the control group contain traditional MCM culture medium, the A solution mother liquor, the B solution mother liquor, the C solution mother liquor and the D solution mother liquor of the improved culture medium of the present invention are respectively stored at 4°C and protected from light for 1 month, and then prepared into an improved MCM medium; traditional MCM culture It was also stored at 4°C and protected from light for 1 month before use; the pH of the two media was adjusted to 7 with a pH meter, and each bottle contained 3L of media;

[0039] (2) Inoculation: On the 1st day...

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Abstract

The invention discloses an improved culture medium applicable to Haematococcus pluvialis vegetative growth and a preparation method of the improved culture medium. The preparation method of the improved culture medium comprises the following steps: firstly, respectively preparing mother liquor of a solution A, mother liquor of a solution B, mother liquor of a solution C and mother liquor of a solution D, wherein the solution A comprises potassium nitrate with the concentration of 200 mg / L, dipotassium phosphate with the concentration of 20 mg / L and magnesium sulfate heptahydrate with the concentration of 100 mg / L; the solution B is anhydrous calcium chloride with the concentration of 40.5 mg / L; the solution C comprises ethylenediamine tetraacetic acid disodium salt with the concentration of 19.8 [mu]g / L and ferric trichloride hexahydrate with the concentration of 24.4 [mu]g / L; the solution D comprises zinc chloride with the concentration of 4.1 [mu]g / L, boric acid with the concentration of 61 [mu]g / L, cobalt chloride hexahydrate with the concentration of 5.1 [mu]g / L, copper sulfate pentahydrate with the concentration of 6.0 [mu]g / L, manganese chloride hexahydrate with the concentration of 4.1 [mu]g / L, and ammonium molybdate tetrahydrate with the concentration of 38 [mu]g / L; sterilized tap water serves as a solvent; secondly, during use, dissolving the fixed amount of the solution A, solution B, solution C and solution D into the solvent in sequence, adjusting the pH value to be 6.9-7.1, and inoculating the culture medium with strains of the Haematococcus pluvialis. The improved MCM culture medium provided by the invention is balanced in nutrition, low in cost, simple to operate during preparation, and high in growth rate and biomass accumulation of cultivated strains, can be stored, and facilitates the improvement of industrialized production efficiency.

Description

technical field [0001] The invention relates to a culture medium suitable for microalgae cultivation and a preparation method, in particular to an improved medium suitable for the vegetative growth of Haematococcus pluvialls and a preparation method, especially to a green culture medium suitable for Haematococcus pluvialls. The improved MCM medium in the vegetative growth stage of cells and its preparation method are suitable for the large-scale cultivation of 712 strains of Haematococcus pluvialis and 797 strains of Haematococcus pluvialls. Background technique [0002] Haematococuus pluvialis is a single-celled freshwater green alga that belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococuaceae, and Haematococcus genus in taxonomy. Under environmental stress conditions, it can rapidly synthesize a red ketone carotenoid astaxanthin, which is recognized as the best organism for producing natural astaxanthin in nature, and its accumulation can reach up to 4% of dry cell...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王巧晗高燕琦陶云莹赫勇宫庆礼
Owner OCEAN UNIV OF CHINA
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