An improved medium suitable for the vegetative growth of Haematococcus pluvialis and its preparation method
A technology of Haematococcus pluvialis and a preparation method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high raw material prices, lack of nutrients, and inability to store, and achieves low cost and balanced nutrition. , the effect of improving production efficiency
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Embodiment 1
[0022] A method for preparing an improved medium suitable for the vegetative growth of Haematococcus pluvialis, comprising the following steps:
[0023] (1) Prepare the mother liquor of solution A: Weigh 20g of potassium nitrate and dissolve it in a small amount of solvent to completely dissolve it; weigh 2g of dipotassium hydrogen phosphate and dissolve it in a small amount of solvent to completely dissolve it; weigh 10g of magnesium sulfate heptahydrate and dissolve it in a small amount of solvent Make it completely dissolved in the solvent; mix the dissolved potassium nitrate solution, dipotassium hydrogen phosphate solution and magnesium sulfate heptahydrate solution evenly, and use the solvent to make the volume to 1L to make A liquid mother liquor, so that the concentration of potassium nitrate is 20g / L, phosphoric acid The concentration of dipotassium hydrogen is 2g / L, and the concentration of magnesium sulfate heptahydrate is 10g / L;
[0024] (2) Preparation of solution...
Embodiment 2
[0032] Embodiment 2: The present invention is now equipped with improved medium to cultivate 712 strains of Haematococcus pluvialis:
[0033] (1) Preparation: 6 5L transparent glass conical flasks that have been autoclaved, 3 5L transparent glass conical flasks of the experimental group contain the improved MCM medium of the present invention, and 3 5L transparent glass conical flasks of the control group contain traditional MCM medium, the pH is adjusted to 7 with a pH meter, and the medium is ready-to-use, and each bottle contains 3L of medium;
[0034] (2) Inoculation: On the 1st day, 712 strains of Haematococcus pluvialis in the exponential growth phase were inoculated respectively in the experimental group and the control group, so that the cell inoculation density in each Erlenmeyer flask was 1.0×10 6 cells / ml;
[0035] (3) Culture: the experimental group and the control group were cultured in the same light incubator, the light-dark cycle was 12:12, the temperature was...
Embodiment 3
[0037] Embodiment 3: The improved culture medium of the present invention stored for 1 month cultivated 712 strains of Haematococcus pluvialis:
[0038] (1) Preparation: 6 5L transparent glass conical flasks that have been autoclaved, 3 5L transparent glass conical flasks of the experimental group contain the improved MCM medium of the present invention, and 3 5L transparent glass conical flasks of the control group contain traditional MCM culture medium, the A solution mother liquor, the B solution mother liquor, the C solution mother liquor and the D solution mother liquor of the improved culture medium of the present invention are respectively stored at 4°C and protected from light for 1 month, and then prepared into an improved MCM medium; traditional MCM culture It was also stored at 4°C and protected from light for 1 month before use; the pH of the two media was adjusted to 7 with a pH meter, and each bottle contained 3L of media;
[0039] (2) Inoculation: On the 1st day...
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