An improved medium suitable for the vegetative growth of Haematococcus pluvialis and its preparation method

Abstract

The invention discloses an improved culture medium applicable to Haematococcus pluvialis vegetative growth and a preparation method of the improved culture medium. The preparation method of the improved culture medium comprises the following steps: firstly, respectively preparing mother liquor of a solution A, mother liquor of a solution B, mother liquor of a solution C and mother liquor of a solution D, wherein the solution A comprises potassium nitrate with the concentration of 200 mg / L, dipotassium phosphate with the concentration of 20 mg / L and magnesium sulfate heptahydrate with the concentration of 100 mg / L; the solution B is anhydrous calcium chloride with the concentration of 40.5 mg / L; the solution C comprises ethylenediamine tetraacetic acid disodium salt with the concentration of 19.8 [mu]g / L and ferric trichloride hexahydrate with the concentration of 24.4 [mu]g / L; the solution D comprises zinc chloride with the concentration of 4.1 [mu]g / L, boric acid with the concentration of 61 [mu]g / L, cobalt chloride hexahydrate with the concentration of 5.1 [mu]g / L, copper sulfate pentahydrate with the concentration of 6.0 [mu]g / L, manganese chloride hexahydrate with the concentration of 4.1 [mu]g / L, and ammonium molybdate tetrahydrate with the concentration of 38 [mu]g / L; sterilized tap water serves as a solvent; secondly, during use, dissolving the fixed amount of the solution A, solution B, solution C and solution D into the solvent in sequence, adjusting the pH value to be 6.9-7.1, and inoculating the culture medium with strains of the Haematococcus pluvialis. The improved MCM culture medium provided by the invention is balanced in nutrition, low in cost, simple to operate during preparation, and high in growth rate and biomass accumulation of cultivated strains, can be stored, and facilitates the improvement of industrialized production efficiency.

Description

technical field

[0001] The invention relates to a culture medium suitable for microalgae cultivation and a preparation method, in particular to an improved medium suitable for the vegetative growth of Haematococcus pluvialls and a preparation method, especially to a green culture medium suitable for Haematococcus pluvialls. The improved MCM medium in the vegetative growth stage of cells and its preparation method are suitable for the large-scale cultivation of 712 strains of Haematococcus pluvialis and 797 strains of Haematococcus pluvialls. Background technique

[0002] Haematococuus pluvialis is a single-celled freshwater green alga that belongs to Chlorophyta, Chlorophyceae, Volvox, Haematococuaceae, and Haematococcus genus in taxonomy. Under environmental stress conditions, it can rapidly synthesize a red ketone carotenoid astaxanthin, which is recognized as the best organism for producing natural astaxanthin in nature, and its accumulation can reach up to 4% of dry cell...

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