Prebiotics-rich oat peptide and preparation method thereof

Abstract

The invention relates to an oat peptide rich in prebiotics and a preparation method thereof, and belongs to the technical field of biology, the product adopts oat peptide, prebiotics, wall-broken oat powder, white hyacinth bean powder, roxburgh rose powder and lotus seed powder as main raw materials; wherein the oat peptide is the oat peptide which is 5kDa or below and is obtained by sequentially carrying out step-by-step enzymolysis on oat protein through papain, snail protease and nattokinase; the white hyacinth bean powder is an extract obtained by performing common enzymolysis on white hyacinth beans through cellulase and pectinase and then sequentially performing step-by-step enzymolysis on the white hyacinth beans through trypsin and papain; the lotus seed powder is an extract obtained by performing common enzymolysis on peeled lotus seeds through cellulase and pectinase and then sequentially performing step-by-step enzymolysis through fig protease and papain. The product follows the functions of clearing, regulating and nourishing, all aspects are matched in a balanced mode, the digestion and absorption rate is increased, blood toxins and intestinal waste are removed, blood sugar and blood fat are reduced, immunity is improved, mood comfort is facilitated, and nerve soothing and sleep aiding are achieved; the effect is prominent, rapid and safe without side effects.

Description

Technical Field

[0001] The invention belongs to the field of biotechnology, and particularly relates to an oat peptide rich in prebiotics and a preparation method thereof. Background Art

[0002] With the continuous improvement of living standards, unhealthy lifestyles and eating habits have gradually become the norm. High-sugar, high-fat dietary intake, coupled with lack of exercise, has led to an increase in the incidence of chronic diseases such as diabetes and hyperlipidemia year by year. For diabetic patients, stable control of blood sugar is crucial. High blood sugar not only damages various organs of the body, but can also cause a series of serious complications. Similarly, high blood lipids are also a common health problem for modern people. Excessive lipid content in the blood can cause blood vessel blockage and increase the risk of cardiovascular and cerebrovascular diseases. In addition, factors such as work pressure, daily chores, and excessive use of electronic devices have made insomnia and poor sleep quality a common phenomenon. In severe cases, it will also affect the immune system, reduce the body's resistance and ability to prevent diseases.

[0003] Therefore, health products or medicines that lower blood sugar, lower blood lipids, help sleep and improve immunity are of great significance in modern society. They provide people with a convenient and effective way to manage their health, helping them prevent and control chronic diseases, improve sleep quality, and enhance immunity, so that they can better cope with various challenges in life.

[0004] Oats, as a common health food, also have excellent effects. Oats are rich in dietary fiber, especially β-glucan, which can delay the absorption of carbohydrates and reduce the rate of increase in blood sugar after meals, which is very beneficial for controlling blood sugar. At the same time, the dietary fiber in oats can also combine with cholesterol in the intestines, reduce the absorption of cholesterol, and lower blood lipid levels. The nutrients such as B vitamins and minerals in oats can regulate the function of the nervous system, relieve tension, and help improve sleep. Moreover, the antioxidants in oats can remove free radicals in the body, enhance immunity, and make the body healthier.

[0005] However, the effect of simply eating oats is not obvious, and it is difficult to achieve the effect of efficiently and quickly lowering blood sugar, lowering blood lipids, promoting sleep and improving immunity. Summary of the invention

[0006] The existing simple edible oatmeal and other foods have no obvious health improvement effect, and it is difficult to achieve the comprehensive effects of reducing blood sugar, reducing blood lipids, helping sleep and improving immunity efficiently and quickly. The present invention provides an oat peptide rich in prebiotics and a preparation method thereof, nutritionally comprehensive and balanced broken wall oatmeal powder, oat peptides obtained by special enzymatic hydrolysis, a variety of prebiotics for conditioning the intestine, a large amount of strong antioxidant vitamin C, SOD, rich flavonoids (blood scavengers), rich linolenic acid OMG-3, palmitoleic acid OMG-7 and linoleic acid OMG-6 and other unsaturated fatty acids, white lentil powder and lotus seed powder that are beneficial to the spleen, stomach and lungs, follow the principle of clearing, regulating and nourishing and balanced matching in all aspects, improve digestion and absorption rate, remove blood toxins and intestinal waste, reduce blood sugar and blood lipids, improve immunity, help to feel good, calm the nerves and help sleep; the effect is outstanding, fast and safe without side effects. The specific technical scheme is as follows:

[0007] A prebiotic-rich oat peptide comprises the following raw materials in parts by weight: 1-3 parts of oat peptide, 3-10 parts of prebiotic, 50-60 parts of broken oatmeal powder, 5-10 parts of white lentil powder, 5-10 parts of roxburghii powder, and 3-5 parts of lotus seed powder; the oat peptide is an oat peptide below 5 kDa obtained by sequentially enzymolysis of oat protein with papain, snail protease and nattokinase; the white lentil powder is an extract obtained by co-enzymolysis of white lentil with cellulase and pectinase, and then sequentially enzymolysis with trypsin and papain, and then drying it; the lotus seed powder is an extract obtained by co-enzymolysis of peeled lotus seeds with cellulase and pectinase, and then sequentially enzymolysis with fig protease and papain, and then drying it.

[0008] In the above technical solution, the prebiotics include fructooligosaccharides, xylooligosaccharides and inulin, and the mass ratio is: fructooligosaccharides: xylooligosaccharides: inulin = (10-15): (0.5-1.5): (10-15).

[0009] In the above technical scheme, the preparation method of the oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 10 to 12 times the mass of the oat grains, adjusting the pH to 4.5 to 5.0, the temperature to 40°C to 50°C, adding 0.5% to 2% of the mass of the oat grains of cellulase and pectinase respectively, enzymolysis for 3h to 4h, adjusting the pH to 5.5 to 6.0, the temperature to 50°C to 60°C, adding 0.5% to 2% of the mass of the oat grains of amylase, enzymolysis for 2h to 3h, adding 80% to 90% of the mass of the oat grains of sodium chloride, soaking for 3h to 4h; then centrifuging and collecting the supernatant; adding an ammonium sulfate aqueous solution to the supernatant, stirring continuously until the protein is completely The protein is completely precipitated and centrifuged to obtain protein; the protein is resuspended in water 6 to 8 times the mass of the protein, 0.5% to 1.5% of the mass of the protein is added with papain, enzymolysis is carried out at 55 to 60°C for 2h to 3h, the enzyme is inactivated at high temperature, the temperature is reduced to 30 to 40°C, 0.5% to 1.5% of the mass of the protein is added with snail protease, the enzymolysis is carried out at a constant temperature for 1.5h to 2h, the enzyme is inactivated at high temperature, the pH is adjusted to 8.0 to 9.0 and the temperature is 40 to 50°C, 0.5% to 1.5% of the mass of the protein is added with nattokinase, the enzymolysis is carried out at a constant temperature for 1h to 1.5h, and the enzyme is inactivated at high temperature; after the temperature is reduced to room temperature, filtration is carried out using a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, and freeze-dried to obtain oat peptide.

[0010] In the above technical solution, the oat peptides include at least the following four peptides, namely Pep1, Pep2, Pep3 and Pep4;

[0011] The amino acid sequence of Pep1, SEQ ID NO: 1, is DVNNS;

[0012] The amino acid sequence of Pep2, SEQ ID NO: 2, is ANEL;

[0013] The amino acid sequence of Pep3, SEQ ID NO: 3, is EPRAKP;

[0014] The amino acid sequence of Pep4, SEQ ID NO: 4, is VPRGW;

[0015] The amino acid sequence of Pep5, SEQ ID NO:5, is GGDRPTYL;

[0016] The content of Pep1 is 2.8 wt% or more, the content of Pep2 is 3.5 wt% or more, the content of Pep3 is 4.1 wt% or more, the content of Pep4 is 4.8 wt% or more, and the content of Pep5 is 2.5 wt% or more.

[0017] In the above technical solution, the concentration of the ammonium sulfate aqueous solution is 4 mol / L to 6 mol / L; the rotation speed of the centrifugal separation is 4000 r / min to 8000 r / min, and the centrifugal separation time is 10 min to 20 min.

[0018] In the above technical scheme, the preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water 10 to 12 times the mass of white lentil, adjusting the pH to 4.5 to 5.0, the temperature to 40°C to 50°C, adding 0.5% to 2% of the mass of cellulase and pectinase of white lentil respectively, enzymolysis for 3h to 4h, adjusting the pH to 7.8 to 8.5, adding 0.5% to 1.5% of the mass of trypsin of white lentil, enzymolysis at 36°C to 38°C for 60min to 80min, inactivating the enzyme at high temperature, adjusting the pH to 6.5 to 7.0, the temperature to 55°C to 60°C, adding 0.5% to 1.5% of the mass of papain of white lentil, enzymolysis for 100min to 120min, inactivating the enzyme at high temperature, filtering with a 200-mesh to 300-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

[0019] In the above technical scheme, the preparation method of the lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water 10 to 12 times the mass of the lotus seeds, adjusting the pH to 4.5 to 5.0, the temperature to 40°C to 50°C, adding 0.5% to 2% of the mass of cellulase and pectinase respectively, enzymolysis for 3h to 4h, adjusting the pH to 5.5 to 6.0, the temperature to 55°C to 65°C, adding 0.5% to 1.5% of the mass of ficin by the mass of the lotus seeds, enzymolysis for 2h to 3h, inactivating the enzyme at high temperature, adjusting the pH to 6.5 to 7.0, the temperature to 55°C to 60°C, adding 0.5% to 1.5% of the mass of papain by the mass of the lotus seeds, enzymolysis for 100min to 120min, inactivating the enzyme at high temperature, filtering with a 200-mesh to 300-mesh sieve to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

[0020] In the above technical solution, the temperature of high temperature enzyme inactivation is 85°C to 95°C, and the time of high temperature enzyme inactivation is 10min to 30min.

[0021] In the above technical scheme, the preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder;

[0022] In the above technical scheme, the preparation method of the roxburghii powder comprises the following steps: taking roxburghii fruits, drying them, and then grinding and crushing them to obtain roxburghii powder.

[0023] The method for preparing the above-mentioned oat peptide rich in prebiotics comprises the following steps:

[0024] According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

[0025] Compared with the prior art, the oat peptide rich in prebiotics and the preparation method thereof of the present invention have the following beneficial effects:

[0026] 1. The oat peptide of the present invention is an oat peptide of less than 5kDa obtained by enzymatic hydrolysis of oat protein by papain, snail protease and nattokinase. Oat peptide can stimulate pancreatic β cells to increase the secretion of insulin, and insulin can promote the uptake and utilization of glucose by tissues and reduce blood sugar levels. It improves the body's sensitivity to insulin, makes it easier for cells to uptake and utilize glucose, and improves the state of insulin resistance. Oat peptide can regulate the synthesis and metabolism of cholesterol and triglycerides in the liver, promote the conversion and excretion of cholesterol, and reduce the content of lipids in the blood. It combines with fat in the intestine to reduce fat absorption, thereby reducing blood lipid levels. Oat peptide has a certain antioxidant capacity, can reduce the damage of free radicals to vascular endothelial cells, prevent lipid peroxidation, and help maintain the normal function of blood vessels. Oat peptide can stimulate the proliferation and activity of immune cells such as macrophages, lymphocytes, etc., and enhance the body's immune function. It regulates the secretion of immune-related factors and coordinates the functions of the immune system.

[0027] 2. Prebiotics include oligofructose, oligoxylose and inulin. Prebiotics can promote the growth of beneficial bacteria and inhibit the reproduction of harmful bacteria. Metabolites such as short-chain fatty acids produced by beneficial bacteria can improve the intestinal environment, affect the intestinal absorption of glucose, and lower blood sugar levels. By regulating the intestinal flora, it affects the secretion of hormones such as incretin by intestinal endocrine cells, improves insulin sensitivity, and promotes blood sugar regulation. Prebiotics can bind to bile acids in the intestine, reduce the reabsorption of bile acids, and prompt the liver to use cholesterol to synthesize new bile acids, thereby reducing the cholesterol content in the blood. Good intestinal function helps the absorption of nutrients and the discharge of metabolic waste, and reduces the interference of physical discomfort on sleep. Prebiotics indirectly create conditions for good sleep by regulating intestinal flora and improving intestinal function. Beneficial bacteria can stimulate the intestinal mucosal immune system, enhance the activity of immune cells, and improve the body's immunity. In the process of metabolizing prebiotics, intestinal flora will produce some substances with immunomodulatory effects, such as short-chain fatty acids, vitamins, etc., to enhance the body's immune function.

[0028] 3. Cracked oatmeal powder contains high levels of dietary fiber such as β-glucan, which can delay the digestion and absorption of carbohydrates and reduce the rate of increase in blood sugar after a meal. At the same time, dietary fiber can increase satiety, reduce food intake, and help control blood sugar. Dietary fiber can bind to cholesterol in the intestines, reduce cholesterol absorption, and promote its excretion from the body. Dietary fiber regulates lipid metabolism, affects the activity of lipid metabolism-related enzymes in the liver, reduces the synthesis of triglycerides and low-density lipoprotein cholesterol, and increases high-density lipoprotein cholesterol levels. Cracked oatmeal powder contains a variety of vitamins and minerals, such as B vitamins, which help maintain the normal function of the nervous system, relieve anxiety and tension, and promote sleep.

[0029] 4. The white lentil powder of the present invention is an extract obtained by enzymolysis of white lentil with cellulase and pectinase, and then enzymolysis of trypsin and papain step by step, and the white lentil powder is obtained after drying. White lentil powder can stimulate pancreatic β cells to secrete insulin, increase the content of insulin, thereby promoting the uptake and utilization of glucose by tissues and lowering blood sugar levels. Certain components in white lentil powder can bind to cholesterol, prevent the absorption of cholesterol in the intestine, and reduce the cholesterol content in the blood. It promotes the excretion of bile acid, thereby prompting the liver to synthesize new bile acid using cholesterol and lowering the cholesterol level in the blood. White lentil powder contains some components that regulate the nervous system, help relax the body and mind, and promote sleep; by regulating the body's physiological functions, such as improving digestive function, stabilizing blood sugar, etc., it creates a suitable internal body environment for good sleep. It regulates the secretion of immune-related factors, coordinates the functions of the immune system, and improves the body's immunity.

[0030] 5. Rosa roxburghii powder contains flavonoids, vitamins and other ingredients, which can promote glucose utilization and glycogen synthesis by regulating the activity of enzymes related to sugar metabolism, and lower blood sugar levels; it helps to improve insulin sensitivity, enhance the uptake and utilization of glucose by cells, and improve insulin resistance. The active ingredients can inhibit the synthesis of cholesterol in the liver and reduce the cholesterol content in the blood; it promotes the excretion of bile acid, thereby prompting the liver to use cholesterol to synthesize new bile acid and lower the cholesterol level in the blood. It is rich in antioxidants, such as vitamin C and flavonoids, which can reduce the damage of free radicals to immune cells and maintain the normal function of immune cells.

[0031] 6. The lotus seed powder of the present invention is an extract obtained by enzymolyzing peeled lotus seeds with cellulase and pectinase, and then enzymolyzing them step by step with ficin and papain, and then drying them to obtain the lotus seed powder.

[0032] Lotus seed powder can affect the activity of certain enzymes related to sugar metabolism, such as inhibiting α-glucosidase, reducing the digestion and absorption rate of carbohydrates, and thus reducing the increase in blood sugar after a meal. Lotus seed powder ingredients can prevent the intestinal absorption of cholesterol by binding to cholesterol, thereby reducing the cholesterol content in the blood; affecting the activity of lipid metabolism-related enzymes in the liver, promoting the conversion and excretion of cholesterol, and reducing triglyceride levels. Lotus seed powder contains some ingredients with sedative and tranquilizing effects, such as liensinine, which can act on the nervous system, regulate the secretion of neurotransmitters, relieve anxiety and tension, and promote sleep. Lotus seed powder is rich in antioxidants, such as polyphenol compounds, which can reduce the damage of free radicals to immune cells and maintain the normal function of immune cells.

[0033] 7. The broken oatmeal powder itself is nutritionally comprehensive and balanced, and with the oat peptides obtained by special enzymatic hydrolysis, it contains a variety of prebiotics for regulating the intestines, a large amount of strong antioxidant vitamin C, SOD, rich flavonoids (blood scavengers), rich linolenic acid OMG-3, palmitoleic acid OMG-7 and linoleic acid OMG-6 and other unsaturated fatty acids, sea buckthorn powder, white lentil powder and lotus seed powder that are beneficial to the spleen, stomach and lungs, follows the principle of clearing, regulating and nourishing, and is balanced in all aspects, improves digestion and absorption rate, removes blood toxins and intestinal waste, lowers blood sugar and blood lipids, improves immunity, is conducive to a good mood, calms the nerves and helps sleep; the effect is outstanding, fast and safe without side effects, and has good practical value. DETAILED DESCRIPTION

[0034] The present invention is further described below in conjunction with specific implementation cases, but the present invention is not limited to these embodiments.

[0035] Example 1

[0036] A prebiotic-rich oat peptide comprises the following raw materials in parts by mass: 2 parts of oat peptide, 6 parts of prebiotics, 55 parts of broken-wall oatmeal powder, 8 parts of white lentil powder, 7 parts of roxburghii powder, and 4 parts of lotus seed powder; the prebiotics comprise oligofructose, oligoxylose and inulin, and the mass ratio of oligofructose: oligoxylose: inulin is 12:1:14.

[0037] The preparation method of oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 11 times the mass of the oat grains, adjusting the pH to 4.8 and the temperature to 45°C, respectively adding 1.2% of the mass of cellulase and pectinase of the oat grains, enzymolysis for 3.5 hours, adjusting the pH to 5.8 and the temperature to 55°C, adding 1.2% of the mass of amylase of the oat grains, enzymolysis for 2.5 hours, adding 85% of the mass of sodium chloride of the oat grains, soaking for 3.5 hours; then centrifuging at 6000r / min for 15 minutes, collecting the supernatant; adding 5mo l / L ammonium sulfate aqueous solution, continuously stirring until the protein is completely precipitated, centrifuging at 6000r / min for 15min to obtain the protein; resuspending the protein in water 7 times the weight of the protein, adding papain at 1% of the weight of the protein, enzymolysis at 58°C for 2.5h, inactivating the enzyme at high temperature, reducing the temperature to 35°C, adding snail protease at 1% of the weight of the protein, enzymolysis at a constant temperature for 1.5h, inactivating the enzyme at high temperature, adjusting the pH to 8.5 and the temperature to 45°C, adding nattokinase at 1% of the weight of the protein, enzymolysis at a constant temperature for 1h, inactivating the enzyme at high temperature; after reducing the temperature to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, freeze-drying, and obtaining oat peptides.

[0038] The preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water 11 times the mass of white lentil, adjusting pH 4.8, temperature 45°C, adding 1.5% of cellulase and pectinase by mass of white lentil respectively, enzymolysis for 3.5h, adjusting pH 8.1, adding 1.2% of trypsin by mass of white lentil, enzymolysis at 37°C for 70min, high temperature inactivation at 90°C for 20min, adjusting pH 6.8, temperature 58°C, adding 1% of papain by mass of white lentil, enzymolysis for 110min, high temperature inactivation at 90°C for 20min, filtering with a 250-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

[0039] The preparation method of lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water of 11 times the mass of the lotus seeds, adjusting the pH to 4.8 and the temperature to 45 DEG C, respectively adding 1.2% of cellulase and pectinase by mass of the lotus seeds, performing enzymolysis for 3.5 hours, adjusting the pH to 5.8 and the temperature to 60 DEG C, adding 1% of fig protease by mass of the lotus seeds, performing enzymolysis for 2.5 hours, inactivating the enzyme at 90 DEG C for 20 minutes, adjusting the pH to 6.8 and the temperature to 58 DEG C, adding 1% of papain by mass of the lotus seeds, performing enzymolysis for 110 minutes, inactivating the enzyme at 90 DEG C for 20 minutes, filtering with a 250-mesh screen to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

[0040] The preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder.

[0041] The preparation method of the roxburghii powder comprises the following steps: drying the roxburghii fruit, and then grinding and crushing the roxburghii fruit to obtain the roxburghii powder.

[0042] The method for preparing the above-mentioned oat peptide rich in prebiotics comprises the following steps:

[0043] According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

[0044] Example 2

[0045] A prebiotic-rich oat peptide comprises the following raw materials in parts by mass: 1 part of oat peptide, 3 parts of prebiotics, 50 parts of broken-wall oatmeal powder, 5 parts of white lentil powder, 5 parts of roxburghii powder, and 3 parts of lotus seed powder; the prebiotics comprise oligofructose, oligoxylose and inulin, and the mass ratio of the prebiotics is: oligofructose: oligoxylose: inulin=10:0.5:10.

[0046] The preparation method of oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 10 times the mass of the oat grains, adjusting the pH to 4.5 and the temperature to 40°C, respectively adding 0.5% of the mass of cellulase and pectinase of the oat grains, enzymolysis for 3 hours, adjusting the pH to 5.5 and the temperature to 50°C, adding 0.5% of the mass of amylase of the oat grains, enzymolysis for 2 hours, adding 80% of the mass of sodium chloride of the oat grains, and soaking for 3 hours; then centrifuging at 4000r / min for 10 minutes, collecting the supernatant; adding 4mo l / L ammonium sulfate aqueous solution, continuously stirring until the protein is completely precipitated, centrifuging at 4000r / min for 10min to obtain the protein; resuspending the protein in water 6 times the mass of the protein, adding papain at 0.5% of the mass of the protein, enzymolysis at 55°C for 2h, inactivating the enzyme at high temperature, reducing the temperature to 30°C, adding snail protease at 0.5% of the mass of the protein, enzymolysis at constant temperature for 1.5h, inactivating the enzyme at high temperature, adjusting the pH to 8.0 and the temperature to 40°C, adding nattokinase at 0.5% of the mass of the protein, enzymolysis at constant temperature for 1h, inactivating the enzyme at high temperature; after reducing the temperature to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, freeze-drying, and obtaining oat peptides.

[0047] The preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water 10 times the mass of white lentil, adjusting pH 4.5, temperature 40 DEG C, adding 0.5% of cellulase and pectinase by mass of white lentil respectively, enzymolysis for 3 hours, adjusting pH 7.8, adding 0.5% of trypsin by mass of white lentil, enzymolysis at 36 DEG C for 60 minutes, high temperature inactivation at 85 DEG C for 10 minutes, adjusting pH 6.5, temperature 55 DEG C, adding 0.5% of papain by mass of white lentil, enzymolysis for 100 minutes, high temperature inactivation at 85 DEG C for 10 minutes, filtering with a 200-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

[0048] The preparation method of lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water of 10 times the mass of the lotus seeds, adjusting the pH to 4.5 and the temperature to 40 DEG C, respectively adding 0.5% of cellulase and pectinase by mass of the lotus seeds, performing enzymolysis for 3 hours, adjusting the pH to 5.5 and the temperature to 55 DEG C, adding 0.5% of fig protease by mass of the lotus seeds, performing enzymolysis for 2 hours, inactivating the enzyme at 85 DEG C for 10 minutes, adjusting the pH to 6.5 and the temperature to 55 DEG C, adding 0.5% of papain by mass of the lotus seeds, performing enzymolysis for 100 minutes, inactivating the enzyme at 85 DEG C for 10 minutes, filtering with a 200-mesh screen to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

[0049] The preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder.

[0050] The preparation method of the roxburghii powder comprises the following steps: drying the roxburghii fruit, and then grinding and crushing the roxburghii fruit to obtain the roxburghii powder.

[0051] The method for preparing the above-mentioned oat peptide rich in prebiotics comprises the following steps:

[0052] According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

[0053] Example 3

[0054] A prebiotic-rich oat peptide comprises the following raw materials in parts by mass: 1 part of oat peptide, 10 parts of prebiotics, 50 parts of broken-wall oatmeal powder, 10 parts of white lentil powder, 5 parts of roxburghii powder, and 5 parts of lotus seed powder; the prebiotics comprise oligofructose, oligoxylose and inulin, and the mass ratio of the prebiotics is: oligofructose: oligoxylose: inulin=10:1.5:10.

[0055] The preparation method of oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 12 times the mass of the oat grains, adjusting the pH to 4.5 and the temperature to 50°C, respectively adding 0.5% of the mass of cellulase and pectinase of the oat grains, enzymolysis for 4 hours, adjusting the pH to 5.5 and the temperature to 60°C, adding 0.5% of the mass of amylase of the oat grains, enzymolysis for 3 hours, adding 80% of the mass of sodium chloride of the oat grains, and soaking for 4 hours; then centrifuging at 4000r / min for 20 minutes, collecting the supernatant; adding 4mo l / L ammonium sulfate aqueous solution, continuously stirring until the protein is completely precipitated, centrifuging at 8000r / min for 10min to obtain the protein; resuspending the protein in water 8 times the mass of the protein, adding papain at 0.5% of the mass of the protein, enzymolysis at 60°C for 2h, inactivating the enzyme at high temperature, reducing the temperature to 40°C, adding snail protease at 0.5% of the mass of the protein, enzymolysis at a constant temperature for 2h, inactivating the enzyme at high temperature, adjusting the pH to 8.0 and the temperature to 50°C, adding nattokinase at 0.5% of the mass of the protein, enzymolysis at a constant temperature for 1.5h, inactivating the enzyme at high temperature; after reducing the temperature to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, freeze-drying, and obtaining oat peptides.

[0056] The preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water 10 times the mass of white lentil, adjusting pH 5.0, temperature 40°C, adding 2% of cellulase and pectinase by mass of white lentil respectively, enzymolysis for 3 hours, adjusting pH 8.5, adding 0.5% of trypsin by mass of white lentil, enzymolysis at 38°C for 60 minutes, high temperature inactivation at 95°C for 10 minutes, adjusting pH 7.0, temperature 55°C, adding 1.5% of papain by mass of white lentil, enzymolysis for 100 minutes, high temperature inactivation at 95°C for 10 minutes, filtering with a 300-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

[0057] The preparation method of lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water of 10 times the mass of the lotus seeds, adjusting the pH to 5.0 and the temperature to 40 DEG C, respectively adding 2% of the mass of cellulase and pectinase of the lotus seeds, performing enzymolysis for 3 hours, adjusting the pH to 6.0 and the temperature to 55 DEG C, adding 1.5% of the mass of ficin of the lotus seeds, performing enzymolysis for 2 hours, inactivating the enzyme at 95 DEG C for 10 minutes, adjusting the pH to 7.0 and the temperature to 55 DEG C, adding 1.5% of the mass of papain of the lotus seeds, performing enzymolysis for 100 minutes, inactivating the enzyme at 95 DEG C for 10 minutes, filtering with a 300-mesh screen to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

[0058] The preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder.

[0059] The preparation method of the roxburghii powder comprises the following steps: drying the roxburghii fruit, and then grinding and crushing the roxburghii fruit to obtain the roxburghii powder.

[0060] The method for preparing the above-mentioned oat peptide rich in prebiotics comprises the following steps:

[0061] According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

[0062] Example 4

[0063] A prebiotic-rich oat peptide comprises the following raw materials in parts by mass: 3 parts of oat peptide, 10 parts of prebiotics, 60 parts of broken-wall oatmeal powder, 10 parts of white lentil powder, 10 parts of roxburghii powder, and 5 parts of lotus seed powder; the prebiotics comprise fructooligosaccharides, xylo-oligosaccharides, and inulin, and the mass ratio of fructooligosaccharides: xylo-oligosaccharides: inulin is 15:1.5:15.

[0064] The preparation method of oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 12 times the mass of the oat grains, adjusting the pH to 5.0 and the temperature to 50°C, respectively adding 2% of the mass of cellulase and pectinase of the oat grains, enzymolysis for 4 hours, adjusting the pH to 6.0 and the temperature to 60°C, adding 2% of the mass of amylase of the oat grains, enzymolysis for 3 hours, adding 90% of the mass of sodium chloride of the oat grains, and soaking for 4 hours; then centrifuging at 8000r / min for 20 minutes, collecting the supernatant; adding 6mo l / L ammonium sulfate aqueous solution, continuously stirring until the protein is completely precipitated, centrifuging at 8000r / min for 20min to obtain the protein; resuspending the protein in water 8 times the mass of the protein, adding papain at 1.5% of the mass of the protein, enzymolysis at 60°C for 3h, inactivating the enzyme at high temperature, reducing the temperature to 40°C, adding snail protease at 1.5% of the mass of the protein, enzymolysis at a constant temperature for 2h, inactivating the enzyme at high temperature, adjusting the pH to 9.0 and the temperature to 50°C, adding nattokinase at 1.5% of the mass of the protein, enzymolysis at a constant temperature for 1.5h, inactivating the enzyme at high temperature; after reducing the temperature to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, freeze-drying, and obtaining oat peptides.

[0065] The preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water 12 times the mass of white lentil, adjusting pH 5.0, temperature 50°C, adding 2% of cellulase and pectinase by mass of white lentil respectively, enzymolysis for 4 hours, adjusting pH 8.5, adding 1.5% of trypsin by mass of white lentil, enzymolysis at 38°C for 80 minutes, high temperature inactivation at 95°C for 30 minutes, adjusting pH 7.0, temperature 60°C, adding 1.5% of papain by mass of white lentil, enzymolysis for 120 minutes, high temperature inactivation at 95°C for 30 minutes, filtering with a 300-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

[0066] The preparation method of lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water of 12 times the mass of the lotus seeds, adjusting the pH to 5.0 and the temperature to 50 DEG C, respectively adding 2% of the mass of cellulase and pectinase of the lotus seeds, performing enzymolysis for 4 hours, adjusting the pH to 6.0 and the temperature to 65 DEG C, adding 1.5% of the mass of ficin of the lotus seeds, performing enzymolysis for 3 hours, inactivating the enzyme at 95 DEG C for 30 minutes, adjusting the pH to 7.0 and the temperature to 60 DEG C, adding 1.5% of the mass of papain of the lotus seeds, performing enzymolysis for 120 minutes, inactivating the enzyme at 95 DEG C for 30 minutes, filtering with a 300-mesh screen to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

[0067] The preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder.

[0068] The preparation method of the roxburghii powder comprises the following steps: drying the roxburghii fruit, and then grinding and crushing the roxburghii fruit to obtain the roxburghii powder.

[0069] The method for preparing the above-mentioned oat peptide rich in prebiotics comprises the following steps:

[0070] According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

[0071] Example 5

[0072] A prebiotic-rich oat peptide comprises the following raw materials in parts by mass: 3 parts of oat peptide, 3 parts of prebiotic, 60 parts of broken wall oatmeal powder, 5 parts of white lentil powder, 10 parts of roxburghii powder, and 3 parts of lotus seed powder; the prebiotic comprises fructooligosaccharide, xylo-oligosaccharide and inulin, and the mass ratio of fructooligosaccharide: xylo-oligosaccharide: inulin is 15:0.5:15.

[0073] The preparation method of oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 10 times the mass of the oat grains, adjusting the pH to 5.0 and the temperature to 40°C, respectively adding 2% of the mass of cellulase and pectinase of the oat grains, enzymolysis for 3 hours, adjusting the pH to 6.0 and the temperature to 50°C, adding 2% of the mass of amylase of the oat grains, enzymolysis for 2 hours, adding 90% of the mass of sodium chloride of the oat grains, and soaking for 3 hours; then centrifuging at 8000r / min for 10 minutes, collecting the supernatant; adding 6mo l / L ammonium sulfate aqueous solution, continuously stirring until the protein is completely precipitated, centrifuging at 4000r / min for 20min to obtain the protein; resuspending the protein in water 6 times the mass of the protein, adding papain at 1.5% of the mass of the protein, enzymolysis at 55°C for 3h, inactivating the enzyme at high temperature, cooling to 30°C, adding snail protease at 1.5% of the mass of the protein, enzymolysis at constant temperature for 1.5h, inactivating the enzyme at high temperature, adjusting the pH to 9.0 and the temperature to 40°C, adding nattokinase at 1.5% of the mass of the protein, enzymolysis at constant temperature for 1h, inactivating the enzyme at high temperature; after cooling to room temperature, filtering with a 5kDa ultrafiltration membrane to obtain a filtrate below 5kDa, freeze-drying, and obtaining oat peptides.

[0074] The preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water 12 times the mass of white lentil, adjusting pH 4.5, temperature 50°C, adding 0.5% of cellulase and pectinase by mass of white lentil respectively, enzymolysis for 4 hours, adjusting pH 7.8, adding 1.5% of trypsin by mass of white lentil, enzymolysis at 36°C for 80 minutes, high temperature inactivation at 85°C for 30 minutes, adjusting pH 6.5, temperature 60°C, adding 0.5% of papain by mass of white lentil, enzymolysis for 120 minutes, high temperature inactivation at 85°C for 30 minutes, filtering with a 200-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

[0075] The preparation method of lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water of 12 times the mass of the lotus seeds, adjusting the pH to 4.5 and the temperature to 50 DEG C, respectively adding 0.5% of cellulase and pectinase by mass of the lotus seeds, performing enzymolysis for 4 hours, adjusting the pH to 5.5 and the temperature to 65 DEG C, adding 0.5% of fig proteinase by mass of the lotus seeds, performing enzymolysis for 3 hours, performing enzyme inactivation at 85 DEG C for 30 minutes, adjusting the pH to 6.5 and the temperature to 60 DEG C, adding 0.5% of papain by mass of the lotus seeds, performing enzymolysis for 120 minutes, performing enzyme inactivation at 85 DEG C for 30 minutes, filtering with a 200-mesh screen to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

[0076] The preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder.

[0077] The preparation method of the roxburghii powder comprises the following steps: drying the roxburghii fruit, and then grinding and crushing the roxburghii fruit to obtain the roxburghii powder.

[0078] The method for preparing the above-mentioned oat peptide rich in prebiotics comprises the following steps:

[0079] According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

[0080] The raw materials such as oat peptides, prebiotics, broken oatmeal powder, white lentil powder, roxburghii powder, lotus seed powder, etc. prepared in the above embodiments are produced in excess (large quantities).

[0081] Comparative Example 1

[0082] No oat peptide was added, and the oat peptide content was replaced by cracked oatmeal powder; other parameters and methods were the same as in Example 1.

[0083] Comparative Example 2

[0084] In the preparation method of oat peptide, snail protease hydrolysis is not used; other parameters and methods are the same as in Example 1.

[0085] Comparative Example 3

[0086] In the preparation method of oat peptide, nattokinase enzymatic hydrolysis is not used; other parameters and methods are the same as in Example 1.

[0087] Comparative Example 4

[0088] In the preparation method of oat peptide, snail protease and nattokinase are not used for enzymatic hydrolysis; other parameters and methods are the same as those in Example 1.

[0089] Comparative Example 5

[0090] The white lentil flour is prepared by directly grinding white lentils into powder; other parameters and methods are the same as those in Example 1.

[0091] Comparative Example 6

[0092] In the preparation method of white lentil flour, papain enzymolysis is not used; other parameters and methods are the same as in Example 1.

[0093] Comparative Example 7

[0094] The lotus seed powder is prepared by directly grinding lotus seeds into powder; other parameters and methods are the same as those in Example 1.

[0095] Comparative Example 8

[0096] In the preparation method of lotus seed powder, ficin hydrolysis is not used; other parameters and methods are the same as in Example 1.

[0097] The oat peptide rich in prebiotics (hereinafter referred to as sample) prepared in the above-mentioned embodiments and comparative examples was tested.

[0098] 1. Toxicity testing

[0099] Healthy mice were selected and divided into 13 groups, each with 6 mice; each group was gavaged with the samples of each embodiment and each comparative example, with a gavage volume of 0.5 g / kg·bw, twice a day, and the physical signs were observed. Result: Negative, belonging to the non-toxic grade.

[0100] Healthy mice were selected and divided into 39 groups, each with 6 mice, as test groups (corresponding to 3 doses of each sample); samples of each embodiment and each comparative example were taken, and three dose groups were set at 0.8g / kg, 1.5g / kg, and 3g / kg; a blank control group (6 mice) without adding samples was set; the sample samples were mixed into the basic feed and fed to mice for 30 consecutive days, and the symptoms of poisoning and death were observed every day, and the health status was tested at the end of the experiment. The result indicators were all within the normal range, safe and non-toxic.

[0101] 2. Blood sugar lowering test

[0102] The high-sugar feed formula is 65% basic feed + 22% sucrose + 10% lard + 2% cholesterol + 1% sodium cholate. The basic feed formula is 73% corn, 20% wheat bran, 5% fish meal, 1.5% cereal powder, and 0.5% salt. A 1% concentration of streptozotocin solution is prepared using a pH 4 citric acid-sodium citrate buffer.

[0103] Select several SPF rats and feed them with basic feed for one week at a temperature of 25°C and a relative humidity of 45%. Then, perform the following operations: (1) Take 6 rats and feed them with basic feed for 4 weeks, fast for 12 hours, and inject 0.38g / kg of physiological saline intraperitoneally as a normal control group; (2) Take several rats and feed them with high-sugar and high-fat feed for 4 weeks, fast for 12 hours, and inject 1% streptozotocin solution intraperitoneally at 25mg / kg to prepare a rat diabetes model; After continuing to feed high-sugar feed for one week after injection, measure fasting blood sugar, and blood sugar>15mmo l / L is considered to be a successful model. The rats with successful modeling are divided into 13 groups, each with 6 rats, corresponding to the test groups of each embodiment and each comparative example; another 6 rats with successful modeling are taken as the model group.

[0104] Normal control group: 0.25g / kg normal saline was administered daily and the rats were fed with basic feed. Experimental group: The rats were administered with the samples of each embodiment and each comparative example respectively, with a daily oral administration of 0.25g / kg and the rats were fed with high-sugar feed. Model group: 0.25g / kg normal saline was administered daily and the rats were fed with high-sugar feed.

[0105] The rats were fasted but not watered for 12 hours 1 day before oral administration and on the 15th day after oral administration, and then blood was collected and their fasting blood glucose levels were measured using a blood glucose analyzer. The test results are shown in Table 1 below.

[0106] Table 1 Hypoglycemic test results (average value)

[0107]

[0108] As can be seen from the above results, the samples prepared in Examples 1 to 5 have a good hypoglycemic effect. Among them, as can be seen from the results of Comparative Example 1, oat peptides have a certain hypoglycemic effect, and the effect of not adding oat peptides on hypoglycemic effect is reduced. As can be seen from the results of Comparative Examples 2 to 4, the oat peptides obtained by changing the preparation method of oat peptides and omitting the fermentation or enzymolysis step have a decreased effect on hypoglycemic effect. As can be seen from the results of Comparative Examples 5 to 6, white lentil powder prepared by different methods has different degrees of hypoglycemic effect, and white lentil powder is enzymolyzed by trypsin and papain step by step, and the hypoglycemic effect of the obtained product is more obvious. As can be seen from the results of Comparative Examples 7 to 8, lotus seed powder prepared by different methods has different degrees of hypoglycemic effect, and lotus seed powder is enzymolyzed by ficin and papain step by step, and the hypoglycemic effect of the obtained product is more obvious.

[0109] 3. Lipid-lowering test

[0110] Several SPF rats were selected and divided into 15 groups (6 rats in each group); they were normal control group, high-fat control group and test group (corresponding to the samples of each embodiment and each comparative example group, respectively). The normal group was fed only with basic feed every day (the basic feed formula was 73% corn, 20% wheat bran, 5% fish meal, 1.5% cereal powder and 0.5% salt); the high-fat control group was fed only with high-fat feed every day (the high-fat feed formula was 12% sucrose, 12% egg yolk powder, 15% lard, 3% cholesterol, 0.2% sodium cholate, and the remainder was basic feed). The test group was fed with high-fat feed every day, and then 0.25g / kg of the sample was administered by gavage every day. After 30 days of continuous feeding, the rats were killed, and the contents of total cholesterol (TC) and triglyceride (TG) in the rat serum were determined after blood was collected from the peritoneal vein. The test results are shown in Table 2 below.

[0111] Table 2 Results of lipid-lowering test (average value)

[0112]

[0113] It can be seen from the above results that the samples prepared in Examples 1 to 5 have a good lipid-lowering effect. Among them, it can be seen from the results of Comparative Example 1 that oat peptides have a certain lipid-lowering effect, and the effect of not adding oat peptides on lipid-lowering is reduced. It can be seen from the results of Comparative Examples 2 to 4 that the preparation method of oat peptides is changed, and the oat peptides obtained after omitting the fermentation or enzymolysis step have a decreased effect on lipid-lowering. It can be seen from the results of Comparative Examples 5 to 6 that white lentil powder prepared by different methods has different degrees of lipid-lowering effect, and white lentil powder is enzymolyzed by trypsin and papain step by step, and the lipid-lowering effect of the obtained product is more obvious. It can be seen from the results of Comparative Examples 7 to 8 that lotus seed powder prepared by different methods has different degrees of lipid-lowering effect, and lotus seed powder is enzymolyzed by fig proteinase and papain step by step, and the lipid-lowering effect of the obtained product is more obvious.

[0114] 4. Sleep aid test

[0115] Several BALB / C mice weighing 18g to 22g were selected. After adaptive feeding for 1 week, they were divided into 16 groups, with 6 mice in each group; they were respectively a normal control group gavaged with 250mg / kg.bw distilled water and a test group gavaged with 220mg / kg.bw sample (corresponding to the samples of each embodiment and comparative example respectively); each group was gavaged once a day for 30 consecutive days. 1 hour after the last gavage of each group of mice, 62mg / kg.bw of sodium pentobarbital was intraperitoneally injected; after the intraperitoneal injection of sodium pentobarbital, the mouse's righting reflex disappeared and entered a sleep state, and the sleep ended when the mouse's righting reflex recovered. Compare the sleep time of mice in each group: the time from the disappearance of the righting reflex to the recovery is defined as the sleep time of the mouse. The results are shown in Table 3.

[0116] Table 3 Sleep aid test results (average value)

[0117] Group Sleep time Group Sleep time Normal control group 41′36″ Comparative Example 1 54′21″ Example 1 55′24″ Comparative Example 2 55′16″ Example 2 52′12″ Comparative Example 3 55′33″ Example 3 60′35″ Comparative Example 4 54′43″ Example 4 58′41″ Comparative Example 5 49′52″ Example 5 49′58″ Comparative Example 6 50′38″ Comparative Example 7 48′26″ Comparative Example 8 49′29″

[0118] It can be seen from the above results that the samples prepared in Examples 1 to 5 have a certain sleep-aiding effect. Among them, it can be seen from the results of Comparative Example 1 that oat peptides have little effect on the sleep-aiding effect. It can be seen from the results of Comparative Examples 5 to 6 that white lentil powder prepared by different methods has different degrees of sleep-aiding effect, and the white lentil powder is enzymolyzed by trypsin and papain step by step, and the sleep-aiding effect of the obtained product is more obvious. It can be seen from the results of Comparative Examples 7 to 8 that lotus seed powder prepared by different methods has different degrees of sleep-aiding effect, and the lotus seed powder is enzymolyzed by ficin and papain step by step, and the sleep-aiding effect of the obtained product is more obvious.

[0119] 5. Tests to improve immunity

[0120] A number of female ICR mice weighing 18 g to 22 g were selected and adaptively raised for 3 days; they were randomly divided into 6 groups, namely a normal control group gavaged with 0.5 mL of distilled water every day, a model control group gavaged with 0.5 mL of distilled water every day, and a test group gavaged with 220 mg / kg.bw of the sample every day (corresponding to the samples of each embodiment and comparative example, respectively).

[0121] Except for the normal control group, the other groups were given cyclophosphamide 40 mg / kg intraperitoneal injection for two consecutive days after continuous gavage for 3 weeks for immunosuppression. After continuous gavage for 4 weeks, 1 ml of whole blood was collected from each mouse, and the total white blood cell count and lymphocyte count were detected by a complete blood cell analyzer. The test results are shown in Table 4 below.

[0122] Table 4 Immunity enhancement test results (average value)

[0123]

[0124] It can be seen from the above results that the samples prepared in Examples 1 to 5 have a good effect of improving immunity. Among them, it can be seen from the results of Comparative Example 1 that oat peptides have a certain effect of improving immunity, and the effect of improving immunity is reduced without adding oat peptides. It can be seen from the results of Comparative Examples 2 to 4 that the oat peptides obtained by changing the preparation method of oat peptides and omitting the fermentation or enzymolysis step have a reduced effect on improving immunity. It can be seen from the results of Comparative Examples 5 to 6 that white lentil powder prepared by different methods has different degrees of improving immunity, and white lentil powder is enzymolyzed by trypsin and papain step by step, and the obtained product has a more obvious effect of improving immunity. It can be seen from the results of Comparative Examples 7 to 8 that lotus seed powder prepared by different methods has different degrees of improving immunity, and lotus seed powder is enzymolyzed by fig proteinase and papain step by step, and the obtained product has a more obvious effect of improving immunity.

[0125] VI. The oat peptide components prepared in the above-mentioned embodiments and comparative examples 2 to 4 were subjected to peptide chain amino acid sequence detection using a mass spectrometer to obtain the following results of distinguishing the amino acid sequences of oat peptides, as shown in Table 5 below.

[0126] Table 5 Oat peptide test results

[0127]

[0128] Among them, the amino acid sequence of Pep1 SEQ ID NO: 1 is DVNNS; the amino acid sequence of Pep2 SEQ ID NO: 2 is ANEL; the amino acid sequence of Pep3 SEQ ID NO: 3 is EPRAKP; the amino acid sequence of Pep4 SEQ ID NO: 4 is VPRGW; the amino acid sequence of Pep5 SEQ ID NO: 5 is GGDRPTYL;

[0129] From the above results, it can be seen that the oat peptide components obtained by omitting the enzymatic hydrolysis step are different, and the above-mentioned 5 small and medium-molecule peptides cannot be obtained, which has a certain impact on the effect of the product.

Claims

1. An oat peptide rich in prebiotics, characterized in that: The invention comprises the following raw materials in parts by weight: 1 to 3 parts of oat peptides, 3 to 10 parts of prebiotics, 50 to 60 parts of broken-wall oatmeal powder, 5 to 10 parts of white lentil powder, 5 to 10 parts of roxburghii powder, and 3 to 5 parts of lotus seed powder; The oat peptides are oat peptides below 5kDa obtained by enzymatically hydrolyzing oat protein with papain, snail protease and nattokinase in sequence; the white lentil powder is an extract obtained by enzymatically hydrolyzing white lentil with cellulase and pectinase, and then enzymatically hydrolyzing it with trypsin and papain in sequence, and the white lentil powder is obtained after drying; the lotus seed powder is an extract obtained by enzymatically hydrolyzing peeled lotus seeds with cellulase and pectinase, and then enzymatically hydrolyzing them with fig protease and papain in sequence, and the lotus seed powder is obtained after drying.

2. The oat peptide rich in prebiotics according to claim 1, characterized in that: The prebiotics include fructooligosaccharides, xylooligosaccharides and inulin, and the mass ratio is: fructooligosaccharides: xylooligosaccharides: inulin = (10-15): (0.5-1.5): (10-15).

3. The oat peptide rich in prebiotics according to claim 1, characterized in that: The preparation method of oat peptide comprises the following steps: taking shelled oat grains, crushing them, adding water 10 to 12 times the mass of the oat grains, adjusting the pH to 4.5 to 5.0 and the temperature to 40°C to 50°C, respectively adding 0.5% to 2% of the mass of cellulase and pectinase of the oat grains, performing enzymolysis for 3h to 4h, adjusting the pH to 5.5 to 6.0 and the temperature to 50°C to 60°C, adding 0.5% to 2% of the mass of amylase of the oat grains, performing enzymolysis for 2h to 3h, adding 80% to 90% of the mass of sodium chloride of the oat grains, and soaking for 3h to 4h; then centrifuging and collecting the supernatant; adding an ammonium sulfate aqueous solution to the supernatant, and continuously stirring until the protein is completely precipitated. The protein is separated by centrifugation to obtain the protein; the protein is resuspended in water 6 to 8 times the mass of the protein, 0.5% to 1.5% of the mass of the protein is added with papain, enzymolysis is carried out at 55 to 60°C for 2 to 3 hours, the enzyme is inactivated by high temperature, the temperature is reduced to 30 to 40°C, 0.5% to 1.5% of the mass of the protein is added with snail protease, the enzymolysis is carried out at a constant temperature for 1.5 to 2 hours, the enzyme is inactivated by high temperature, the pH is adjusted to 8.0 to 9.0, the temperature is 40 to 50°C, 0.5% to 1.5% of the mass of the protein is added with nattokinase, the enzymolysis is carried out at a constant temperature for 1 hour to 1.5 hours, and the enzyme is inactivated by high temperature; after the temperature is reduced to room temperature, filtration is carried out using a 5 kDa ultrafiltration membrane to obtain a filtrate below 5 kDa, and freeze-drying is carried out to obtain oat peptides.

4. The oat peptide rich in prebiotics according to claim 3, characterized in that: The oat peptides include at least the following four peptides, namely Pep1, Pep2, Pep3 and Pep4; The amino acid sequence of Pep1, SEQ ID NO: 1, is DVNNS; The amino acid sequence of Pep2, SEQ ID NO: 2, is ANEL; The amino acid sequence of Pep3, SEQ ID NO: 3, is EPRAKP; The amino acid sequence of Pep4, SEQ ID NO: 4, is VPRGW; The amino acid sequence of Pep5, SEQ ID NO:5, is GGDRPTYL; The content of Pep1 is 2.8 wt% or more, the content of Pep2 is 3.5 wt% or more, the content of Pep3 is 4.1 wt% or more, the content of Pep4 is 4.8 wt% or more, and the content of Pep5 is 2.5 wt% or more.

5. The oat peptide rich in prebiotics according to claim 3, characterized in that: The concentration of the aqueous ammonium sulfate solution is 4 mol / L to 6 mol / L; the rotation speed of the centrifugal separation is 4000 r / min to 8000 r / min, and the time of the centrifugal separation is 10 min to 20 min.

6. The oat peptide rich in prebiotics according to claim 1, characterized in that: The preparation method of white lentil powder comprises the following steps: taking mountain white lentil for crushing, adding water of 10 to 12 times the mass of the white lentil, adjusting the pH value to 4.5 to 5.0, the temperature to 40°C to 50°C, respectively adding 0.5% to 2% of cellulase and pectinase by mass of the white lentil, performing enzymolysis for 3h to 4h, adjusting the pH value to 7.8 to 8.5, adding 0.5% to 1.5% of trypsin by mass of the white lentil, performing enzymolysis at 36°C to 38°C for 60min to 80min, inactivating the enzyme at high temperature, adjusting the pH value to 6.5 to 7.0, the temperature to 55°C to 60°C, adding 0.5% to 1.5% of papain by mass of the white lentil, performing enzymolysis for 100min to 120min, inactivating the enzyme at high temperature, filtering with a 200-mesh to 300-mesh sieve to obtain a filtrate, concentrating, and drying to obtain white lentil powder.

7. The oat peptide rich in prebiotics according to claim 1, characterized in that: The preparation method of lotus seed powder comprises the following steps: taking peeled lotus seeds, crushing them, adding water 10 to 12 times the mass of the lotus seeds, adjusting the pH to 4.5 to 5.0 and the temperature to 40 to 50° C., respectively adding 0.5 to 2% of cellulase and pectinase by mass of the lotus seeds, performing enzymolysis for 3 hours to 4 hours, adjusting the pH to 5.5 to 6.0 and the temperature to 55 to 65° C., adding 0.5 to 1.5% of fig proteinase by mass of the lotus seeds, performing enzymolysis for 2 hours to 3 hours, inactivating the enzyme at high temperature, adjusting the pH to 6.5 to 7.0 and the temperature to 55 to 60° C., adding 0.5 to 1.5% of papain by mass of the lotus seeds, performing enzymolysis for 100 to 120 minutes, inactivating the enzyme at high temperature, filtering with a 200-mesh to 300-mesh sieve to obtain a filtrate, concentrating, and drying to obtain lotus seed powder.

8. The oat peptide rich in prebiotics according to claim 3, 6 or 7, characterized in that: The temperature of the high temperature enzyme inactivation is 85° C. to 95° C., and the time of the high temperature enzyme inactivation is 10 min to 30 min.

9. The oat peptide rich in prebiotics according to claim 1, characterized in that: The preparation method of the cracked oatmeal powder comprises the following steps: taking shelled oat grains, grinding and crushing them, and drying them to obtain cracked oatmeal powder; the preparation method of the roxburghii powder comprises the following steps: taking roxburghii fruits, drying them, and then grinding and crushing them to obtain roxburghii powder.

10. A method for preparing an oat peptide rich in prebiotics, for preparing the oat peptide rich in prebiotics according to claim 1, characterized in that: The preparation method comprises the following steps: According to the mass ratio, prebiotics, cracked oatmeal powder, white lentil powder, sea buckthorn powder and lotus seed powder are evenly mixed, and finally oat peptides are added and evenly mixed.

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