Composition with function of stabilizing skin enzyme activity as well as preparation method and application of composition

Abstract

The invention provides a composition with a function of stabilizing skin enzyme activity as well as a preparation method and application of the composition, and the composition is prepared from the following components in parts by mass: 0.001 to 0.01 part of superoxide dismutase, 0.001 to 0.05 part of nicotinamide adenine dinucleotide, 0.11 to 12 parts of a polypeptide compound a and 0.005 to 0.5 part of a polypeptide compound b. The invention also provides a preparation method of the composition and application of the composition in preparation of skin care products for improving skin enzyme activity, repairing skin and resisting aging. The composition can be prepared into emulsion, solution, paste, gel or spray. The composition can effectively improve the enzyme activity of skin, improve the texture and appearance of the skin and repair the skin, and is beneficial to delaying the aging of the skin at the same time.

Description

Technical Field

[0001] The present invention relates to the technical field of functional skin care products, and in particular to a composition having the function of stabilizing skin enzyme activity, and a preparation method and application thereof. Background Art

[0002] The skin epidermis is the first line of immune defense. The skin enzymes on it (such as collagenase) protect the skin through multiple pathways, such as promoting metabolism, anti-inflammatory and antiviral, enhancing antioxidant capacity, promoting collagen synthesis, improving lymphatic metabolic function and repairing damaged cells. These effects work together to maintain the health of the skin and enhance resistance to the external environment.

[0003] However, when people have bad living and eating habits, over-clean their skin, or use skin care products or cosmetics containing irritating ingredients, they will destroy skin enzymes and greatly reduce the activity of skin enzymes. The decrease in skin enzyme activity will bring about a series of skin problems: (1) causing skin aging, functional decline, and reduced resistance to the external environment; (2) affecting the nerve endings and blood vessel function of the skin, increasing the sensitivity of the skin to external stimuli, making the skin more susceptible to discomfort symptoms such as redness, swelling, itching, and stinging, affecting the quality of life; (3) affecting the normal metabolic process of the skin, especially the renewal and shedding of the stratum corneum; when the stratum corneum cannot be shed normally, it will accumulate on the surface of the skin, making the skin look dull and lifeless.

[0004] Therefore, how to prepare a composition with the function of stabilizing the activity of skin enzymes is of great significance to maintaining skin health. Summary of the invention

[0005] In order to solve the above problems, the present invention provides a composition having the function of stabilizing skin enzyme activity, and a preparation method and application thereof. The composition prepared in the present invention is composed of superoxide dismutase, nicotinamide adenine dinucleotide, polypeptide complex a, and polypeptide complex b. The composition can effectively improve skin enzyme activity, improve skin texture and appearance, repair skin, and at the same time help delay skin aging.

[0006] To achieve the above object, the present invention provides a composition having the function of stabilizing skin enzyme activity. The composition comprises, by weight, 0.001-0.01 parts of superoxide dismutase, 0.001-0.05 parts of nicotinamide adenine dinucleotide, 0.11-12 parts of polypeptide complex a, and 0.005-0.5 parts of polypeptide complex b.

[0007] Preferably, the composition comprises, by weight, 0.003-0.008 parts of superoxide dismutase, 0.005-0.02 parts of nicotinamide adenine dinucleotide, 0.21-10.2 parts of polypeptide complex a, and 0.01-0.22 parts of polypeptide complex b.

[0008] Preferably, the composition comprises, by mass, 0.005 parts of superoxide dismutase, 0.01 parts of nicotinamide adenine dinucleotide, 5.1 parts of polypeptide complex a, and 0.112 parts of polypeptide complex b.

[0009] Optionally, the polypeptide complex a comprises, by weight, 0.05-5.8 parts of oligopeptide-1, 0.05-5.8 parts of oligopeptide-3, and 0.01-0.4 parts of tripeptide-1 copper;

[0010] Preferably, the polypeptide complex a comprises, by weight, 0.1-5 parts of oligopeptide-1, 0.1-5 parts of oligopeptide-3, and 0.05-0.2 parts of tripeptide-1.

[0011] Preferably, the polypeptide complex a comprises 2.5 parts of oligopeptide-1, 2.5 parts of oligopeptide-3 and 0.1 parts of tripeptide-1 copper, calculated by mass.

[0012] Optionally, the polypeptide complex b comprises, by weight, 0.001-0.05 parts of acetyl tetrapeptide-5, 0.001-0.05 parts of acetyl hexapeptide-8, 0.001-0.3 parts of carnosine, 0.001-0.05 parts of palmitoyl tripeptide-1, and 0.001-0.05 parts of palmitoyl tetrapeptide-7;

[0013] Preferably, the polypeptide complex b comprises, by weight, 0.002-0.005 parts of acetyl tetrapeptide-5, 0.002-0.005 parts of acetyl hexapeptide-8, 0.002-0.2 parts of carnosine, 0.002-0.005 parts of palmitoyl tripeptide-1, and 0.002-0.005 parts of palmitoyl tetrapeptide-7.

[0014] Preferably, the polypeptide complex b comprises 0.003 parts of acetyl tetrapeptide-5, 0.003 parts of acetyl hexapeptide-8, 0.1 parts of carnosine, 0.003 parts of palmitoyl tripeptide-1, and 0.003 parts of palmitoyl tetrapeptide-7, calculated by mass.

[0015] The present invention also provides a method for preparing the above-mentioned composition, comprising the following steps:

[0016] S1. stirring and mixing superoxide dismutase, nicotinamide adenine dinucleotide and water to obtain phase A;

[0017] S2. Stir and mix oligopeptide-1, oligopeptide-3, tripeptide-1 copper and water to obtain phase B;

[0018] S3. Acetyl tetrapeptide-5, acetyl hexapeptide-8, carnosine, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and butanediol are stirred and mixed to obtain phase C;

[0019] S4. First, mix phase A and phase B evenly, and then add phase C and mix evenly to obtain a composite.

[0020] Optionally, in the preparation step, the composition is adjusted to pH 5.5 using a pH adjuster, and mixed in a 40° C. water bath for 30 minutes to obtain the composition.

[0021] The present invention also provides the use of any of the above-mentioned compositions, and / or a composition obtained by the preparation method of any of the above-mentioned compositions in cosmetics, health products, and medicines, wherein the application includes the composition being made into an emulsion, solution, paste, gel, or spray formulation.

[0022] The synergistic effect mechanism between the components in this application:

[0023] Superoxide dismutase (SOD) used in this application is an important antioxidant enzyme, the main function of which is to catalyze the superoxide anion free radical (O 2- ) undergoes a disproportionation reaction to generate hydrogen peroxide (H2O2) and oxygen (O2), thereby removing superoxide anion free radicals and protecting cells from oxidative stress damage. Continuously supplementing the human body with SOD has a special anti-aging effect.

[0024] Nicotinamide adenine dinucleotide (NAD + ), is a coenzyme present in living cells, promoting cell metabolism, energy synthesis and cell DNA repair, and has a significant anti-aging effect. At the same time, a large amount of experimental data shows that NAD + It is widely involved in a variety of basic physiological activities in the body, thereby intervening in key cellular functions such as energy metabolism, DNA repair, genetic modification, inflammation, biological rhythms and stress resistance.

[0025] The polypeptide complex a used in the present application includes oligopeptide-1, oligopeptide-3, and tripeptide-1 copper, wherein oligopeptide-1 is an epidermal cell growth factor that can promote cell proliferation and differentiation, and can directly act on basal cells to accelerate the generation of healthy new cells, thereby quickly pushing out the damaged keratinocyte layer to form a healthy, regularly arranged stratum corneum, which can form a good coordination effect with various enzymes in the human body; oligopeptide-3 is a small molecule composed of 2-6 amino acids, which can be directly and effectively absorbed and utilized by cells, quickly replenish collagen, thereby playing a good conditioning role, and promoting metabolism, stimulating cell regeneration, proliferation, and new collagen, with high safety; tripeptide-1 copper has the function of firming the skin and reducing wrinkles, restoring the skin's repair ability, and can increase the production of mucus between skin cells and reduce skin damage.

[0026] The polypeptide complex b used in the present application includes acetyl tetrapeptide-5, acetyl hexapeptide-8, carnosine, palmitoyl tripeptide-1, and palmitoyl tetrapeptide-7, wherein acetyl tetrapeptide-5 is an inhibitor of angiotensin converting enzyme, which can improve the microcirculation of ocular lymph and capillaries, eliminate edema, reduce dark circles, and improve skin elasticity and smoothness; acetyl hexapeptide-8 is a biologically active polypeptide, which can not only reduce existing facial wrinkles, but also effectively prevent the formation of new wrinkles. It reduces muscle contraction by inhibiting the release of neurotransmitter acetylcholine, thereby reducing the formation of dynamic lines and expression lines; carnosine is a multifunctional anti-aging cosmetic raw material with strong antioxidant capacity, which can comprehensively prevent various environmental oxidative factors from damaging cells and significantly delay cell aging and degeneration; palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, when used in combination, can effectively promote the synthesis of collagen and hyaluronic acid in fibroblasts, thereby smoothing static lines, reducing wrinkles, tightening skin, and having significant anti-wrinkle, anti-inflammatory and anti-allergic effects.

[0027] The beneficial effects of the present invention are embodied in:

[0028] (1) The present invention uses superoxide dismutase (SOD) and nicotinamide adenine dinucleotide (NAD + Coenzyme), effectively maintain or promote skin enzyme activity, on the one hand improve skin texture and appearance, on the other hand enhance skin immunity and strengthen skin barrier;

[0029] (2) The present invention adds polypeptide complex and NAD to the composition + Coenzyme complex, including NAD + The coenzyme itself has significant anti-aging properties (NAD + Coenzymes can activate anti-aging proteins that play a key role in cell repair and regeneration; by activating these proteins, NAD +Coenzymes help delay the aging process of the skin), while peptides can promote cell regeneration and repair. The combination of the two can more comprehensively combat the aging process. At the same time, NAD + Coenzymes can also help repair damaged cells and tissues; peptides can directly act on cells, promote cell proliferation and differentiation, thereby accelerating the repair process of damaged tissues, protecting the skin barrier, promoting the repair of inflammatory damage and inhibiting the occurrence of inflammation; peptides can also enhance NAD by regulating cell signal transduction pathways. + The role of coenzymes in cells further stabilizes the active enzymes in the skin to participate in redox reactions, promoting processes such as energy metabolism and DNA repair in cells.

[0030] (3) The polypeptide complex a of the present invention, wherein oligopeptide-1, oligopeptide-3, and tripeptide-1 copper cooperate synergistically, can effectively promote collagen growth, protect the skin barrier, and repair the skin; NAD + The coenzyme and acetyl hexapeptide-8 in polypeptide complex b can synergistically exert a significant anti-wrinkle effect; at the same time, the carnosine in polypeptide complex b has a significant anti-glycation effect, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 can exert anti-inflammatory, skin anti-allergy and skin whitening effects; acetyl tetrapeptide-5 can significantly improve skin elasticity. DETAILED DESCRIPTION

[0031] In order to more clearly illustrate the overall concept of the present invention, a detailed description is given below by way of examples.

[0032] In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Therefore, the protection scope of the present invention is not limited to the specific embodiments disclosed below.

[0033] Unless otherwise specified, the raw materials, reagents or devices used in the following examples can be obtained from conventional commercial sources or can be obtained by existing known methods; unless otherwise specified, the methods used in the examples of the present invention are methods known to those skilled in the art.

[0034] Hereinafter, a composition having the function of stabilizing skin enzyme activity, a preparation method and application thereof in the present invention will be described in detail in combination with exemplary embodiments.

[0035] Example 1 Preparation of the composition

[0036] The preparation method of composition 1# comprises the following steps:

[0037] 1. Weigh each raw material according to the corresponding weight parts;

[0038] 2. Stir and mix 0.05 g of superoxide dismutase, 0.1 g of nicotinamide adenine dinucleotide and 60 g of water to obtain phase A;

[0039] 3. Stir and mix 25 g of oligopeptide-1, 25 g of oligopeptide-3, 1 g of tripeptide-1 copper and 600 g of water to obtain phase B;

[0040] 4. Stir and mix 0.03 g of acetyl tetrapeptide-5, 0.03 g of acetyl hexapeptide-8, 1 g of carnosine, 0.03 g of palmitoyl tripeptide-1, 0.03 g of palmitoyl tetrapeptide-7 and 80 g of butanediol to obtain phase C;

[0041] 5. First, stir phase A and phase B evenly, then add phase C and stir evenly, add (triethanolamine) to adjust the pH value to 5.5, and stir and mix in a water bath at 40°C for 30 minutes to obtain composition 1#.

[0042] The composition having the function of stabilizing the activity of skin enzymes prepared as described above is recorded as composition 1#; according to the preparation method of composition 1#, the component dosage ratio is adjusted to prepare compositions 1#-11# and compositions D1#-D4#. The specific formulas are shown in Table 1, compositions 1#-11# are the example experimental group, and compositions D1#-D4# are the comparative experimental group.

[0043] Table 1. Composition formula

[0044]

[0045]

[0046]

[0047] Example 2

[0048] The composition prepared by the method of Reference Example 1 is different in that Example 2 is set according to the parameter conditions of Composition 2# in Table 1, and other conditions are the same as Example 1, to prepare Composition 2#.

[0049] Example 3

[0050] The composition prepared by the method of Reference Example 1 is different in that Example 3 is set according to the parameter conditions of Composition 3# in Table 1, and other conditions are the same as Example 1, to prepare Composition 3#.

[0051] Example 4

[0052] The composition prepared by the method of Reference Example 1 is different in that Example 4 is set according to the parameter conditions of Composition 4# in Table 1, and other conditions are the same as Example 1, to prepare Composition 4#.

[0053] Example 5

[0054] The composition prepared by the method of Reference Example 1 is different in that Example 5 is set according to the parameter conditions of Composition 5# in Table 1, and other conditions are the same as Example 1, to prepare Composition 5#.

[0055] Example 6

[0056] The composition prepared by the method of reference Example 1 is different in that Example 6 is set according to the parameter conditions of composition 6# in Table 1, and tripeptide 1 and decapeptide 4 replace oligopeptide-1 and oligopeptide-3. The other conditions are the same as those of Example 1, and composition 6# is prepared.

[0057] Example 7

[0058] The composition prepared by the method of reference Example 1 is different in that Example 7 is set according to the parameter conditions of composition 7# in Table 1, and acetyl octapeptide-3 and acetyl heptapeptide-4 replace acetyl tetrapeptide-5 and acetyl hexapeptide-8. The other conditions are the same as in Example 1, and composition 7# is prepared.

[0059] Example 8

[0060] The composition prepared by the method of reference Example 1 is different in that Example 8 is set according to the parameter conditions of composition 8# in Table 1, and palmitoyl tripeptide-5 and palmitoyl pentapeptide-4 replace palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7. The other conditions are the same as in Example 1 to prepare composition 8#.

[0061] Example 9

[0062] The composition prepared by the method of reference Example 1 is different in that Example 9 is set according to the parameter conditions of composition 9# in Table 1, and glutathione and arginine / lysine polypeptide are used to replace carnosine and tripeptide-1 copper. The other conditions are the same as those of Example 1, and composition 9# is prepared.

[0063] Example 10

[0064] The composition prepared by the method of reference Example 1 is different in that Example 10 is set according to the parameter conditions of composition 10# in Table 1, and glutathione, palmitoyl pentapeptide-4, and palmitoyl 5-peptide-1 replace polypeptide complex a, and other conditions are the same as in Example 1 to prepare composition 10#.

[0065] Embodiment 11

[0066] The composition was prepared by the method of reference Example 1, except that Example 11 was set according to the parameter conditions of composition 11# in Table 1, and palmitoyl tripeptide-5, palmitoyl pentapeptide-1, and palmitoyl 5-peptide-4 replaced polypeptide complex b. The other conditions were the same as in Example 1, and composition 11# was prepared.

[0067] Comparative Example D1

[0068] The composition prepared by the method of Reference Example 1 is different in that the comparative example D1 is set according to the parameter conditions of the composition D1# in Table 1, and the polypeptide complex a is not added. The other conditions are the same as those of Example 1, and the composition D1# is prepared.

[0069] Comparative Example D2

[0070] The composition prepared by the method of Reference Example 1 is different in that the comparative example D2 is set according to the parameter conditions of the composition D2# in Table 1, and the polypeptide complex b is not added. The other conditions are the same as those of Example 1, and the composition D2# is prepared.

[0071] Comparative Example D3

[0072] The composition prepared by the method of Reference Example 1 is different in that the comparative example D3 is set according to the parameter conditions of the composition D3# in Table 1, and superoxide dismutase is replaced by catalase. The other conditions are the same as those of Example 1, to prepare the composition D3#.

[0073] Comparative Example D4

[0074] The composition prepared by the method of reference Example 1 is different in that the comparative example D4 is set according to the parameter conditions of the composition D4# in Table 1, and nicotinamide adenine dinucleotide is replaced by polydeoxyribonucleotide. The other conditions are the same as those in Example 1, and the composition D4# is prepared.

[0075] Experimental example

[0076] The compositions prepared in the above examples and comparative examples were respectively subjected to skin enzyme activity test (taking elastase as an example), anti-inflammatory test and anti-aging performance test, and the test methods are as follows:

[0077] 1. Elastase activity inhibition experiment

[0078] Elastase is an enzyme that can degrade skin structural proteins such as collagen and elastin. Its increased activity may cause damage and aging of skin structure, and then lead to problems such as wrinkles and skin sagging. Studies have shown that inhibiting the activity of elastase can help slow down the process of skin aging, maintain the elasticity and firmness of the skin, and help reduce signs of skin aging, such as wrinkles and sagging. By detecting the inhibition rate of elastase, the inhibition effect of the composition 1-11# prepared by the experimental group of the embodiment and the composition D1-D4# prepared by the experimental group of the comparative example on elastase was evaluated, wherein the concentration of the composition was 10%. This experiment uses Congo red-elastin as a substrate, and porcine pancreatic elastase hydrolyzes elastin to make Congo red dye fall off into the solution, and the solution now presents red. After adding the enzyme inhibition sample, the enzyme activity is inhibited, the red color becomes lighter, and the inhibition ability is negatively correlated with the depth of red. The inhibition rate can be indirectly obtained by measuring the absorbance at 495nm using a UV-visible spectrophotometer.

[0079] Detection method: (1) Weigh an appropriate amount of the sample to be tested and dilute it into a 5% aqueous solution with ultrapure water;

[0080] (2) Add appropriate amount of elastase solution to the sample tube and background tube respectively, shake well, and let stand at 37°C for 5 min;

[0081] (3) Add appropriate amount of substrate solution and DMSO solution, shake well and react at 37°C for 30 min, and measure the absorbance at 405 nm;

[0082] (4) Calculate the inhibition rate of elastase activity.

[0083] The results of the inhibition rate test of elastase are shown in Table 2:

[0084] Table 2. Elastase activity inhibition results

[0085] Group Elastase activity inhibition rate (%) Blank Group 0 Composition 1# 93.8 Composition 2# 86.3 Composition 3# 85.2 Composition 4# 81.3 Composition 5# 82.7 Composition 6# 74.3 Composition 7# 73.1 Composition 8# 73.8 Composition 9# 74.6 Composition 10# 70.4 Composition 11# 71.2 Composition D1# 58.3 Composition D2# 55.7 Composition D3# 72.9 Composition D4# 73.6

[0086] Analysis of test results: From the results in Table 2, it can be seen that the components of compositions 1-5# in the present invention are reasonably matched, and have an inhibition rate of more than 80% on elastase, especially the inhibition rate of composition 1-3# on elastase reaches more than 85%, and composition 1# reaches more than 90%, which has a significant effect on anti-aging, indicating that the reasonable distribution ratio of superoxide dismutase, nicotinamide adenine dinucleotide, polypeptide complex a, and polypeptide complex b used in this application produces a significant synergistic effect in inhibiting elastase.

[0087] 2. Anti-inflammatory test:

[0088] The inflammatory factor interleukin IL-6 can cause intracellular inflammation; while vascular endothelial growth factor can promote the proliferation of HaCat cells and repair the skin barrier at an appropriate concentration. The concentration of the prepared HaCat cell suspension is 3×10 5 cell / mL, 100μL / well was placed in a 10-well plate, and cultured at 37°C and 5% CO2 until adhered to the wall. Compositions 1-11# prepared in the example experimental group and compositions D1-D4# prepared in the comparative experimental group were diluted with water, and the concentration of the compositions was diluted to 8%. Then, 3 mg of the above compositions were added to the 10-well plates respectively. After 48 hours of incubation in the incubator, the plates were taken out and centrifuged to remove the precipitate. Through multiple liquid phase protein quantification technology, microspheres with specific fluorescent signals were used to link specific capture antibodies, which were then used to capture different objects to be detected in the solution. According to the intensity of the fluorescent signal, the objects to be detected were quantitatively detected to obtain the concentration of interleukin IL-6. The specific results are shown in Table 3:

[0089] Table 3. Interleukin IL-6 concentration results

[0090]

[0091]

[0092] Analysis of test results: From the anti-inflammatory test results shown in Table 3, it can be seen that the components of compositions 1-5# of the present invention are reasonably matched, and the concentrations of interleukin IL-6 produced are all low, and the concentration of interleukin IL-6 produced by composition 1-3# is even lower, and the concentration of interleukin IL-6 produced by composition 1# is the lowest, indicating that the distribution ratio of superoxide dismutase, nicotinamide adenine dinucleotide, polypeptide complex a, and polypeptide complex b used in this application is reasonable, the inflammatory response to cells is small, and the barrier enhancement ability of skin cells is the strongest.

[0093] 3. Anti-aging performance test:

[0094] Testing method: 150 wrinkle-bearing subjects aged 35-40 with healthy skin were selected and divided into 15 groups, with 10 subjects in each group. The subjects in groups 1-11 used compositions 1-11# respectively, and the subjects in groups 12-15 used compositions D1-D4# respectively.

[0095] Usage: After cleansing, the subject takes 0.5g of the corresponding composition and applies it to the cheek area of ​​3*3cm, washes it off after 10 minutes, covers it once at 9 am and once at 6 pm, and performs the following tests at the same skin location:

[0096] 1) Wrinkle area change test:

[0097] The American facial image analyzer VISIA-CR was used to measure the wrinkle area of ​​the subjects before using cosmetics, which was recorded as the initial value. The wrinkle area was measured again after 7 days of continuous use, which was recorded as the ending value. Finally, the change rate of the wrinkle area was calculated, change rate = (initial value - ending value) / initial value. The larger the change rate, the better the anti-aging effect of the cosmetics.

[0098] 2) Transepidermal water loss (TEWL) change test: The German CK skin tester TewameterTM Hex was used to measure the transepidermal water loss of the test subjects before using the cosmetics, which was recorded as the initial value. After 7 days of continuous use, the transepidermal water loss was measured again, which was recorded as the termination value. Finally, the change rate (%) of the transepidermal water loss (TEWL) was calculated, and the change rate = (initial value - termination value) / initial value. The greater the change rate, the better the repair effect of the cosmetics.

[0099] Table 4. Anti-aging test results

[0100] Composition Wrinkle area change rate % TEWL change rate % Composition 1# 16.88 23.97 Composition 2# 14.32 21.34 Composition 3# 14.21 21.58 Composition 4# 13.73 20.29 Composition 5# 13.25 20.36 Composition 6# 11.78 18.37 Composition 7# 11.23 18.26 Composition 8# 11.52 17.85 Composition 9# 11.65 18.19 Composition 10# 10.27 16.32 Composition 11# 10.38 16.24 Composition D1# 7.69 13.38 Composition D2# 8.23 14.05 Composition D3# 11.63 18.31 Composition D4# 10.87 17.96

[0101] Analysis of test results: It can be seen from Table 4 that the components of compositions 1-5# in the present invention are reasonably matched, and the obtained wrinkle area change rate and TEWL change rate values ​​are relatively large, and in compositions 1-3#, even larger wrinkle area change rates and better TEWL change rate values ​​can be obtained, especially composition 1# has the best values ​​of wrinkle area change rate and TEWL change rate, indicating that the reasonable distribution ratio of superoxide dismutase, nicotinamide adenine dinucleotide, polypeptide complex a, and polypeptide complex b used in the present application produces a significant synergistic effect in anti-aging, and the obtained anti-aging effect is the best.

[0102] The above test results indicate that the composition of the present invention has the functions of stabilizing enzyme activity, anti-inflammation, barrier repair, anti-aging and the like.

[0103] Combined with the above experimental results, it can be seen that the composition of the present invention can achieve excellent effects of stabilizing enzyme activity, anti-inflammation, barrier repair and anti-aging, mainly due to the following reasons: (1) through superoxide dismutase SOD and nicotinamide adenine dinucleotide NAD + Coenzymes effectively maintain or promote the activity of skin enzymes, improving skin texture and appearance on the one hand, and enhancing skin immunity and strengthening the skin barrier on the other hand; (2) Peptides and NAD + Coenzyme complex, including NAD + Coenzymes themselves have significant anti-aging properties, while peptides can promote cell regeneration and repair. The combination of the two can more comprehensively combat the aging process. At the same time, NAD +Coenzymes can also help repair damaged cells and tissues; peptides can directly act on cells to promote cell proliferation and differentiation, thereby accelerating the repair process of damaged tissues, protecting the skin barrier, and preventing inflammation; (3) through oligopeptide-1 and oligopeptide-3, it effectively promotes collagen growth and protects the skin barrier; through nicotinamide adenine dinucleotide (NAD + Coenzyme), acetyl hexapeptide-8 have a significant anti-wrinkle effect; tripeptide-1 copper is used for skin repair; carnosine is used for anti-glycation; palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 are used for anti-inflammatory, anti-allergic and whitening; acetyl tetrapeptide-5 is used to improve skin elasticity.

[0104] The above is only the embodiment of the present application, and the protection scope of the present application is not limited by these specific embodiments, but is determined by the claims of the present application. For those skilled in the art, the present application can have various changes and variations. Any modification, equivalent replacement, improvement, etc. made within the technical ideas and principles of the present application should be included in the protection scope of the present application.

Claims

1. A composition having the function of stabilizing skin enzyme activity, characterized in that: Calculated by weight, the composition comprises 0.001-0.01 parts of superoxide dismutase, 0.001-0.05 parts of nicotinamide adenine dinucleotide, 0.11-12 parts of polypeptide complex a, and 0.005-0.5 parts of polypeptide complex b.

2. The composition according to claim 1, characterized in that Calculated by weight, the composition comprises 0.003-0.008 parts of superoxide dismutase, 0.005-0.02 parts of nicotinamide adenine dinucleotide, 0.21-10.2 parts of polypeptide complex a, and 0.01-0.22 parts of polypeptide complex b.

3. The composition according to claim 1, characterized in that Calculated by weight, the composition includes 0.005 parts of superoxide dismutase, 0.01 parts of nicotinamide adenine dinucleotide, 5.1 parts of polypeptide complex a, and 0.112 parts of polypeptide complex b.

4. The composition according to claim 1, characterized in that Calculated by weight, the polypeptide complex a includes 0.05-5.8 parts of oligopeptide-1, 0.05-5.8 parts of oligopeptide-3, and 0.01-0.4 parts of tripeptide-1 copper.

5. The composition according to claim 1, characterized in that Calculated by weight, the polypeptide complex a includes 0.1-5 parts of oligopeptide-1, 0.1-5 parts of oligopeptide-3, and 0.05-0.2 parts of tripeptide-1 copper; Preferably, the polypeptide complex a comprises 2.5 parts of oligopeptide-1, 2.5 parts of oligopeptide-3 and 0.1 parts of tripeptide-1 copper, calculated by mass.

6. The composition according to claim 1, characterized in that By weight, the polypeptide complex b includes 0.001-0.05 parts of acetyl tetrapeptide-5, 0.001-0.05 parts of acetyl hexapeptide-8, 0.001-0.3 parts of carnosine, 0.001-0.05 parts of palmitoyl tripeptide-1, and 0.001-0.05 parts of palmitoyl tetrapeptide-7.

7. The composition according to claim 1, characterized in that By weight, the polypeptide complex b includes 0.002-0.005 parts of acetyl tetrapeptide-5, 0.002-0.005 parts of acetyl hexapeptide-8, 0.002-0.2 parts of carnosine, 0.002-0.005 parts of palmitoyl tripeptide-1, and 0.002-0.005 parts of palmitoyl tetrapeptide-7; Preferably, the polypeptide complex b comprises, by mass, 0.003 parts of acetyl tetrapeptide-5, 0.003 parts of acetyl hexapeptide-8, 0.1 parts of carnosine, 0.003 parts of palmitoyl tripeptide-1, and 0.003 parts of palmitoyl tetrapeptide-7.

8. A method for preparing a composition having the function of stabilizing skin enzyme activity as claimed in any one of claims 1 to 7, characterized in that: The following steps are involved: S1. stirring and mixing superoxide dismutase, nicotinamide adenine dinucleotide and water to obtain phase A; S2. Stir and mix oligopeptide-1, oligopeptide-3, tripeptide-1 copper and water to obtain phase B; S3. Acetyl tetrapeptide-5, acetyl hexapeptide-8, carnosine, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and butanediol are stirred and mixed to obtain phase C; S4. First, mix phase A and phase B evenly, and then add phase C and mix evenly to obtain a composite.

9. The method for preparing the composition according to claim 8, characterized in that: In the preparation step, the pH value of the composition is adjusted to 5.5 by using a pH adjuster, and the mixture is mixed in a water bath at 40° C. for 30 minutes to obtain the composition.

10. Use of the composition according to any one of claims 1 to 7, and / or the composition obtained by the method for preparing the composition according to any one of claims 8 to 9, in cosmetics, health products, and medicines, wherein the application includes the composition being made into an emulsion, solution, paste, gel, or spray formulation.

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