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Method for increasing yield of flask shaking fermentation euglena gracilis klebs through feeding method

A technology of euglena slender and shake flask fermentation, which is applied in the production field of euglena heterotrophic fermentation, can solve the problems of lack of high-yielding algae strains, and achieve the effect of increasing fermentation intensity and accumulation

Inactive Publication Date: 2018-06-12
QINGDAO XUNON BIOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] (1) Lack of high-yielding algae strains;
[0005] (2) Most research-oriented enterprises and institutions are still in the stage of small-scale experiments;
[0006] (3) The production process urgently needs to be further improved;

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The seed culture medium formula used in this example is: (NH 4 ) 2 SO 4 The concentration is 1.0g / L, the yeast extract concentration is 10.0g / L, KH 2 PO 4 The concentration is 2.0g / L, the glucose concentration is 40g / L, MgSO 4 The concentration is 2.0g / L, CaCl 2 The concentration is 0.255g / L, Na 2 SO 4 The concentration is 14.0g / L, K 2 SO 4 The concentration is 0.65g / L, and the trace element content is 2.0mL / L. Among them, the formula of the trace element solution is: FeSO 4 ·7H 2 O 0.5g / L, MnCl 2 ·4H 2 O 0.82g / L, ZnSO 4 ·7H 2 O 1.40g / L, CoCl 2 ·6H 2 O0.005g / L, NiSO 4 ·6H 2 O 0.05g / L, CuSO 4 ·5H 2 O 0.5g / L, Na 2 MoO 4 ·2H 2 O 0.008g / L.

[0029] The fermentation medium formula is: NH 4 ) 2 SO 4 The concentration is 1.0g / L, KH 2 PO 4 The concentration is 2.0g / L, the yeast extract concentration is 3.0g / L, the corn steep liquor concentration is 4.5g / L, the sodium glutamate concentration is 8.5g / L, the glucose concentration is 50.0g / L, Na 2 SO 4 The concentration is 14.0g / L, MgSO...

Embodiment 2

[0036] The seed culture medium formula used in this example is: (NH 4 ) 2 SO 4 The concentration is 1.0g / L, the yeast extract concentration is 8.0g / L, KH 2 PO 4 The concentration is 2.0g / L, the glucose concentration is 50g / L, Na 2 SO 4 The concentration is 14.0g / L, MgSO 4 The concentration is 2.0g / L, K 2 SO 4 The concentration is 0.65g / L, CaCl 2 The concentration is 0.255g / L, and the trace element content is 2.0mL / L. Among them, the formula of the trace element solution is: ZnSO 4 ·7H 2 O 1.40g / L, MnCl 2 ·4H 2 O 0.82g / L, FeSO 4 ·7H 2 O 0.5g / L, CuSO 4 ·5H 2 O0.5g / L、、CoCl 2 ·6H 2 O 0.005g / L, NiSO 4 ·6H 2 O 0.05g / L, Na 2 MoO 4 ·2H 2 O 0.008g / L.

[0037] The fermentation medium formula is: (NH 4 ) 2 SO 4 The concentration is 1.0g / L, the glucose concentration is 70.0g / L, the yeast extract concentration is 3.0g / L, the corn steep liquor concentration is 4.5g / L, the sodium glutamate concentration is 8.5g / L, KH 2 PO 4 The concentration is 2.0g / L, MgSO 4 The concentration is 2.0g / L, Na 2 SO ...

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Abstract

The invention relates to a method for increasing the yield of flask shaking fermentation euglena gracilis klebs through a feeding method. According to the method, by feeding a mixed nitrogen source, the fermentation level of the euglena gracilis klebs in a shake up flask in the middle and later periods can be greatly improved; by feeding a high-concentration carbon source, the requirement for thecarbon source in the middle and later periods of euglena gracilis klebs flask shaking fermentation can be greatly guaranteed, and single batch fermentative strength in the shake up flask can be improved; in this way, accumulation of euglena gracilis klebs biomass in the flask shaking fermentation process is greatly improved; important reference data is provided for test batch, pilot batch and production of the euglena gracilis klebs, and great significance is achieved for large-scale cultivation of the euglena gracilis klebs.

Description

Technical field [0001] The invention relates to a method for increasing the yield of Euglena gracilis by a flow addition method, belonging to the field of microbial (microalgae) fermentation, and relates to the production of Euglena heterotrophic fermentation. Background technique [0002] Euglena, also known as Euglena, was discovered and named by the famous biologist Levinhoek in the 17th century. It belongs to the Euglena, Euglena, and a single-celled algae in the Euglena order. It contains chlorophyll because it contains chlorophyll. In addition to photosynthesis, it can also use natural organic carbon sources for independent nutrition. It has both animal and plant characteristics. Euglena cells are rich in nutrients: vitamins, mineral nutrients, amino acids, unsaturated fatty acids, chlorophyll, Lutein, zeaxanthin, GABA and other 59 kinds of nutrients necessary for the human body every day are all essential nutrients for maintaining health. Euglena has been approved as a fun...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
CPCC12N1/12
Inventor 王志臻孙发强王珊珊刘珊珊
Owner QINGDAO XUNON BIOLOGICAL ENG
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