A method for improving the production of docosahexaenoic acid by Schizochytrium in shake flask fermentation by fed-batch strategy

Abstract

The invention discloses a method for facilitating DHA (docosahexaenoic acid) generation by performing shaking flask fermentation on schizochytrium through a feeding strategy, and belongs to the field of microbial fermentation. The method comprises the following steps: adopting a culture medium containing microelements as a fermentation medium; during the fermentation process, adding a glucose solution, so as to maintain the glucose content in the fermentation liquor at 30-50 g / L; at the same time, adding 210.0-320.0 g / L of a mixed nitrogen source solution with the volume being 1 / 5-1 / 4 of that of the fermentation liquor once. According to the method, the demand of schizochytrium for a carbon source in the middle and later periods of fermentation can be met through the addition of glucose, and the single batch fermentation strength in a shake flask is improved; the fermentation level of schizochytrium in the shake flask in the middle and later periods can be improved through the addition of the mixed nitrogen source; through the operation, the accumulation of schizochytrium biomass during the shaking flask fermentation process can be improved to a greater extent, and the accumulation of DHA in mycelium is facilitated. The method has great significance for scale DHA cultivation and generation of schizochytrium.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and relates to the production of docosahexaenoic acid, in particular to a method for improving the production of docosahexaenoic acid by fermenting Schizochytrium in shake flasks through fed-batch strategy. Background technique [0002] Studies have found that docosahexaenoic acid (22:6n-3, docosahexaenoic acid, DHA) is one of the main components of the human brain, and its content can account for about 10% of human brain lipids. The presence of inotropic acid can improve the flexibility of the brain to a certain extent and inhibit the aging of the brain. During the fetal period, DHA is needed from the time when the fertilized egg divides in the mother's uterus. Therefore, pregnant women should consume sufficient DHA to promote the development of the fetal brain and the proliferation of brain cells. In today's society, people pay more and more attention to the supplementation of DHA during ...