17 results about "Gluconobacter oxydans" patented technology

The invention relates to the technical field of bacterial cellulose preparation, in particular to a composite flora for producing bacterial cellulose and a preparation method thereof, and the preparation method comprises the following steps: screening gluconobacter oxydans, obtaining culture medium preparation materials and preparation tools, preparing a slant culture medium set, preparing an enriched liquid culture medium set, and preparing composite flora mixed inoculation liquid. The method comprises the following steps: preparing a waste tobacco leaf leaching solution, extracting a proportional leaching solution from the waste tobacco leaf leaching solution according to a preset proportion, obtaining a mixed fermentation culture medium based on a preparation tool and the proportional leaching solution, and carrying out inoculation operation on a composite flora mixed inoculation solution by using the mixed fermentation culture medium to obtain a dry cellulose membrane; calculating the yield of the bacterial cellulose based on the dry cellulose membrane, and completing the preparation of the composite flora for producing the bacterial cellulose based on the yield of the bacterial cellulose. The method can be used for scientifically, efficiently and stably preparing the composite flora.

The present invention discloses the construction and application of a recombinant Gluconobacter oxydans, belonging to the fields of genetic engineering and whole-cell catalysis. The present invention heterologously expresses an epoxide hydrolase from Sphingomonas in Gluconobacter oxydans, and simultaneously utilizes the alcohol and aldehyde dehydrogenases bound to the membrane of Gluconobacter oxydans itself to synthesize R-mandelic acid in a one-step process using styrene oxide as a substrate. The present invention combines the transcriptome data of Gluconobacter oxydans, screens 7 strong promoters using green fluorescent protein as a reporter gene, and determines the strongest promoter P for expressing the epoxide hydrolase gene. 12780 , ultimately significantly increasing the yield of R-mandelic acid.

The present invention discloses a method for improving the yield and production intensity of 1,3-dihydroxyacetone by Gluconobacter oxydans, belonging to the technical field of fermentation engineering. In the present invention, dehydrogenase genes that potentially affect the metabolic flux of 1,3-dihydroxyacetone are knocked out in Gluconobacter oxydans to enhance the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G. oxydans WSH-003, the yields, conversion rates, and production intensities of 1,3-dihydroxyacetone of the recombinant strains G. oxydans WSH-1, G. oxydans WSH-2, G. oxydans WSH-3, G. oxydans WSH-4, G. oxydans WSH-5, G. oxydans WSH-6, G. oxydans WSH-7, and G. oxydans WSH-8 are all significantly improved.

The present invention discloses a Gluconobacter oxydans strain tolerant to high concentrations of mandelic acid and its application, belonging to the fields of microbial engineering technology and genetic engineering. In the present invention, Gluconobacter oxydoacetici 621H is used as the starting strain, and Gluconobacter oxydoacetici is subjected to adaptive evolution by gradually adding mandelic acid to the culture medium. The evolved strain has significantly improved tolerance to mandelic acid. Applying the strain Gluconobacter oxydans STA to catalyze the synthesis of R-mandelic acid from styrene oxide, both the yield and the conversion rate are significantly improved. Using the genetically engineered Gluconobacter oxydans strain constructed by the present invention to catalyze the synthesis of R-mandelic acid from styrene oxide, the final yield reaches 14.06 g / L, while that of the wild strain WT is only 10.26 g / L; the catalytic efficiency is also greatly improved, increasing from 0.366 g / L / h to 0.703 g / L / h.

The present application relates to the field of microbial technology, and particularly to a strain of Gluconobacter oxydans and its application. Gluconobacter oxydans The present application provides a strain of Gluconobacter oxydans (Gluconobacter oxydans) named KUST4611, which has been preserved in the Guangdong Microbial Culture Collection Center on October 22, 2025, with a preservation number of GDMCC NO: 67143. The volatile substances produced by the strain provided by the present application during growth have a bacteriostatic effect. When the strain is applied to prepare a fresh-keeping gel, the volatile substances produced by the gel placed in a closed space can achieve good fresh-keeping effect, effectively reduce the occurrence of diseases, maintain the freshness of the peppers, and effectively prolong the fresh-keeping period of the peppers. Meanwhile, the preparation method of the fresh-keeping gel is simple, and the fresh-keeping effect can be easily and conveniently achieved, the cumbersome steps of postharvest preservation are reduced, and the postharvest preservation efficiency is further improved.

The invention relates to the technical field of biological pharmacy, and discloses a mutant strain for efficient biotransformation synthesis of miglitol, a screening method and application. A compound mutation strategy of ARTP mutagenesis and NTG mutagenesis is utilized, a rapid screening method based on carbonyl color development is combined, a mutant strain capable of efficiently converting and synthesizing a miglittol key intermediate is obtained, the mutant strain is named as Gluconobacter oxydans G1036, and the enzyme activity of the mutant strain is 61.7 U / mL and is improved by about 48% compared with that of an original strain. A process for producing 6NSL by catalyzing NEHG through resting cells of the mutant strain is researched, 73.53 g / L of 6NSL is produced by catalyzing 80 g / L of a substrate within 48 h, and compared with 80.37% of an original strain, the 6NSL is improved by 19.63%. According to the present invention, the research on the process for repeatedly and catalytically producing the 6NSL by using the resting cells of the mutant strain in multiple batches shows the good catalytic performance, and 3 batches can be efficiently utilized to accumulatively produce 220.59 g / L of the 6NSL within 144 h;

The present invention discloses a method for improving the yield and production intensity of 1,3-dihydroxyacetone by Gluconobacter oxydans, belonging to the technical field of fermentation engineering. By knocking out dehydrogenase genes in Gluconobacter oxydans that potentially affect the metabolic flux of 1,3-dihydroxyacetone, the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone is enhanced, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G. oxydans WSH-003, the yields, conversion rates and production intensities of 1,3-dihydroxyacetone of the recombinant strains G. oxydans WSH-1, G. oxydans WSH-2, G. oxydans WSH-3, G. oxydans WSH-4, G. oxydans WSH-5, G. oxydans WSH-6, G. oxydans WSH-7 and G. oxydans WSH-8 are all significantly improved.

The invention discloses a gluconobacter oxydans strain, a saccharomycetes strain and application of the gluconobacter oxydans strain and the saccharomycetes strain in tea enzyme products. The invention provides a Gluconobacter oxydans (Gluconobacter oxydans) strain A21, a Starmeria davenportii (Starmeria davenportii) strain YW1 and a microbial agent containing the Gluconobacter oxydans (Gluconobacter oxydans) strain A21 and the Starmeria davenportii strain YW1. The bacterial strain and the microbial agent do not generate pellicle when the tea enzyme is produced by fermenting sugar water, the pellicle does not need to be removed subsequently, the content of polyphenol and organic acid in the obtained tea enzyme is high, and the product yield is high.

The application discloses a kind of for waste PET depolymerization system glycol directional efficient conversion of glycolic acid two enzyme level connection system and its application, belong to biological catalysis and resource recycling technical field.The system includes a kind of mutant alcohol dehydrogenase with high selectivity to glycol and a mutant aldehyde dehydrogenase ALDH-V7.Said mutant alcohol dehydrogenase is obtained in at least one combination mutation in 97 and 101 and / or 145 and 148 in the basis of ADH5 enzyme derived from acetic acid bacillus ( Gluconobacter oxydans ).The system can efficiently and directionally oxidize low-value glycol in waste PET chemical or biological depolymerization product to high-value-added product glycolic acid under mild conditions, effectively avoiding peroxidation and C-C bond rupture.The application provides an efficient and green biological catalysis solution for upgrading and recycling of waste PET, and realizes a circular economy path of "waste plastics-degradable plastic monomers".

The present invention discloses a method for improving 1,3-dihydroxyacetone yield and production intensity of Gluconobacter oxydans, and belongs to the field of fermentation engineering technology. The present invention enhances the efficiency of converting its substrate glycerol into 1,3-dihydroxyacetone by knocking out the dehydrogenase gene that has a potential impact on the metabolic flux of 1,3-dihydroxyacetone in Gluconobacter oxydans, thereby improving the yield and production intensity of 1,3-dihydroxyacetone. Compared with the control strain G.oxydansWSH-003, the 1,3-dihydroxyacetone yield, conversion rate and production intensity of recombinant strains G.oxydans WSH-1, G.oxydans WSH-2, G.oxydans WSH-3, G.oxydans WSH-4, G.oxydans WSH-5, G.oxydans WSH-6, G.oxydans WSH-7 and G.oxydans WSH-8 are significantly improved.

The invention belongs to the technical field of microorganisms, and particularly relates to gluconobacter oxydans and application thereof. The strain ZLV2-2 is classified and named as Gluconobacter oxydans, the Gluconobacter oxydans is preserved in China General Microbiological Culture Collection Center (CGMCC) on October 29, 2025, and the preservation number is CGMCC NO.36411. The strain ZLV2-2 is named as Gluconobacter oxydans. The Gluconobacter oxydans ZLV2-2 provided by the invention has strong genetic stability, can efficiently ferment and convert isopentenol to synthesize isopentenoic acid, has a wide action substrate spectrum, also has a good conversion effect on primary alcohols such as n-propanol and n-butanol, and has industrial application potential; the method is simple in production process, mild in condition, green and safe, avoids the problems of high energy consumption and environmental pollution of a traditional chemical method, has few by-products, and is beneficial to extraction and separation operation.

The invention discloses a method for improving acid production of cells through cooperation of diatom and gluconobacter oxydans, and belongs to the technical field of bioengineering. The diatom and the gluconobacter oxydans are simultaneously inoculated into a sugar-containing liquid culture medium and are aerated and stirred, and then fermentation is performed. According to the method, the gluconic acid or the xylonic acid is efficiently prepared through biotransformation of the diatom and the gluconobacter oxydans, the yield of the gluconic acid or the xylonic acid added with the diatom under the same condition is obviously higher than that of the gluconic acid or the xylonic acid not added with the diatom in each time period, efficient acid production of cells is achieved, and the method has good innovativeness and practical value.

The invention discloses a method for improving styrene stress tolerance of gluconobacter oxydans through overexpression gene marR, and belongs to the technical field of gene engineering and microbial engineering. According to the invention, through overexpression of a MarR family transcriptional regulation factor marR (GOXRS11840) in the gluconobacter oxydans, the tolerance of the gluconobacter oxydans to styrene stress is obviously improved; the survival rate of the recombinant gluconobacter oxydans prepared by the method is 1.6 times of that of a gluconobacter oxydans control strain after the recombinant gluconobacter oxydans are subjected to stress culture for 10 hours in an environment of 15g / L styrene.

The invention discloses a method for improving the styrene stress resistance of gluconobacter oxydans, and belongs to the technical field of genetic engineering and microbial engineering. According to the method, firstly, styrene is added into a culture medium in a gradient mode, adaptive evolution of wild type gluconobacter oxydans to styrene is carried out, and the finally obtained evolved strain ST remarkably improves the tolerance of the gluconobacter oxydans to styrene. Secondly, flagellin FlgE is knocked out from the gluconobacter oxydans, so that the stress ability of the gluconobacter oxydans to styrene is also improved; the growth performance of the gene-deleted gluconobacter oxydans ST-delta flgE prepared by the method disclosed by the invention is far higher than that of wild type gluconobacter oxydans after the gene-deleted gluconobacter oxydans ST-delta flgE is cultured for 10 hours under 15g / L styrene.

The application discloses a method for improving the tolerance of Gluconobacter oxydans to phenylethylene stress by overexpressing a gene marR and belongs to the technical field of genetic engineering and microbial engineering. The application significantly improves the tolerance of Gluconobacter oxydans to phenylethylene stress by overexpressing a MarR family transcriptional regulator marR (GOX_RS11840) in Gluconobacter oxydans. The survival rate of the recombinant Gluconobacter oxydans prepared by the method of the application after stress culture for 10 hours in an environment with 15 g / L phenylethylene is 1.6 times that of a control strain of Gluconobacter oxydans.

The application discloses a method for improving the anti-styrene stress ability of Gluconobacter oxydans, and belongs to the technical field of genetic engineering and microbial engineering. Firstly, the adaptability evolution of wild-type Gluconobacter oxydans to styrene is carried out by gradient addition of styrene in a culture medium, and finally, the evolved strain ST significantly improves the tolerance of Gluconobacter oxydans to styrene. Secondly, the knocking out of flagellin FlgE in Gluconobacter oxydans also improves the stress ability of Gluconobacter oxydans to styrene; the growth performance of the gene deletion Gluconobacter oxydans ST-ΔflgE prepared by the method of the application after 10h culture under 15g / L styrene is far better than that of the wild-type Gluconobacter oxydans.

The application discloses a gluconobacter oxydans for leaching waste printed circuit boards and a leaching method thereof, and belongs to the technical field of microbial resource recycling and treatment, wherein a leaching solution for leaching and extraction comprises the gluconobacter oxydans, metabolites of the gluconobacter oxydans and an H2O2 solution, and the percentage of metal leaching and the leaching time are shortened by adding the H2O2 solution. Compared with traditional chemical and biological leaching, the application realizes efficient leaching of copper in the waste printed circuit boards under relatively mild conditions, is more safe, and can reduce secondary pollution to the environment.