Method for producing bacterial cellulose with wheat straws/maize straws

A bacterial cellulose and corn stalk technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, low cellulose production and yield

Active Publication Date: 2010-07-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the popularization and application of bacterial cellulose, problems such as high cost, low cellulose yield and yield have become the main limiting factors for its industrial production and popularization and application.

Method used

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  • Method for producing bacterial cellulose with wheat straws/maize straws
  • Method for producing bacterial cellulose with wheat straws/maize straws

Examples

Experimental program
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Effect test

Embodiment 1

[0066] Wheat straw is first ground with a plant pulverizer, then soaked overnight (12-24h) with dilute sulfuric acid (3%, w / v) in a reactor at a solid / liquid ratio of 1:6 between the straw and dilute acid solution, Then react at a temperature of 121° C. for 60 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.

[0067] Add NaOH to adjust the pH value of the hydrolyzed liquid to about 10, filter the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 30°C water bath for 12-24 overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilut...

Embodiment 2

[0069] Wheat straw is first ground with a plant grinder, and then soaked overnight (12-24h) in a reactor with dilute hydrochloric acid (1%, w / v) at a solid / liquid ratio of 1:10 straw to dilute acid solution, Then react at a temperature of 121° C. for 75 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.

[0070] Add Ca(OH) 2Adjust the pH value of the hydrolyzed liquid to about 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 40°C water bath for 12-24 overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute ...

Embodiment 3

[0072] Wheat straw is first ground with a plant pulverizer, and then soaked overnight (12-24h) with dilute sulfuric acid (2%, w / v) in a reactor with a solid / liquid ratio of 1:15 straw to dilute acid solution, Then react at a temperature of 100° C. for 80 minutes, then separate the straw residue and acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4° C. for later use.

[0073] Add 25% ammonia water to adjust the pH value of the hydrolyzed liquid to 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and fine-tune the pH value to 10. Then seal it with a film, place it in a 25°C water bath to react overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (at room temperature for 5-10min), filter out the activated carbon with filter paper to obtain detoxified hydrolyzed clear liquid, and fine-tune the pH value to 5.5 with dilute s...

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Abstract

The invention relates to a method for producing bacterial cellulose with wheat straws / maize straws, comprising the following steps: (1) grinding the wheat straws or maize straws, soaking the ground wheat straws or maize straws in dilute sulfuric acid or hydrochloric acid to react, then separating the residues of the wheat straws or maize straws from the acid hydrolysate through pump filtration and collecting the hydrolysate for later use; (2) detoxicating the hydrolysate; and (3) taking the detoxicated hydrolysate as the carbon source in the culture medium and adding the nitrogen source to prepare the culture medium and inoculating acetobacter aceti or gluconobacter oxydans into a shaker at 25-30 DEG C and 160-250r / min to be cultured or an incubator at 25-30 DEG C to undergo static culture, thus obtaining the bacterial cellulose. The carbon source in the culture medium is good in quality, low in price and suitable for industrial production.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose preparation, in particular to a method for producing bacterial cellulose by utilizing wheat straw / corn stalk. Background technique [0002] Bacterial Cellulose (BC) is also known as Microbial Cellulose. Compared with other higher plant cellulose in nature, it has many unique properties, including high purity, high crystallinity, and high degree of polymerization. , high water holding capacity, high tensile strength, strong biological adaptability, etc., can be widely used in artificial skin and blood vessels, medical dressings, adhesives, audio equipment vibration membranes, papermaking, textiles, composite membranes and other fields. Biomaterials with promising commercial applications. However, in the popularization and application of bacterial cellulose, problems such as high cost, low cellulose output and yield have become the main limiting factors for its industrial production and populariz...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/02C12R1/01
Inventor 洪枫杨雪霞杨光
Owner DONGHUA UNIV
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