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Method for producing bacterial cellulose with wheat straws/spruces

A bacterial cellulose and wheat straw technology, applied in the field of bacterial cellulose preparation, can solve the problem of high cost of bacterial cellulose

Inactive Publication Date: 2010-07-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glucose, mannitol, etc. are commonly used carbon sources in the preparation of bacterial cellulose, all of which are commercial reagents. The use of these commercial carbon sources makes the cost of bacterial cellulose higher

Method used

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  • Method for producing bacterial cellulose with wheat straws/spruces
  • Method for producing bacterial cellulose with wheat straws/spruces

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] The wheat straw is ground with a plant pulverizer, and then soaked overnight (12-24h) in the reactor with dilute sulfuric acid (3%, w / v) at a solid / liquid ratio of wheat straw to dilute acid solution of 1:6. Then react at 121°C for 60 minutes, and then separate the straw residue and acid solution by suction, collect the hydrolysate, and refrigerate in a refrigerator at 4°C for later use.

[0072] Add NaOH to adjust the pH of the supernatant hydrolysate to about 10, filter out the precipitate with filter paper to obtain the treated hydrolysate, and then fine-tune the pH to 10.0. Then it was sealed with a membrane and placed in a 30°C water bath to react for 12-24 overnight. Finally, the pH of the hydrolysate was adjusted to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (5-10min at room temperature), filter out the activated carbon with filter paper to obtain the detoxified hydrolyzed liquid, and then fine-tune the pH value to 5.5 with dilute sul...

Embodiment 2

[0074] The wheat straw is ground with a plant pulverizer, and then soaked overnight (12-24h) in the reactor with a solid / liquid ratio of the wheat straw to the dilute acid solution with dilute hydrochloric acid (1%, w / v). Then react at 121°C for 75 minutes, and then separate the straw residue and acid liquid by suction filtration, collect the hydrolysate, and refrigerate in a refrigerator at 4°C for later use.

[0075] Add Ca(OH) 2 Adjust the pH of the supernatant hydrolysate to about 10, filter out the precipitate with filter paper to obtain the treated hydrolysate, and then fine-tune the pH to 10.0. Then it was sealed with a film and placed in a 40°C water bath to react for 12-24 overnight. Finally, the pH of the hydrolysate was adjusted to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (5-10min at room temperature), filter out the activated carbon with filter paper to obtain the detoxified hydrolyzed liquid, and then fine-tune the pH value to 5.5 wi...

Embodiment 3

[0077] The wheat straw is ground with a plant pulverizer, and then soaked overnight (12-24h) in the reactor with dilute sulfuric acid (2%, w / v) at a solid / liquid ratio of 1:15. Then react at a temperature of 100°C for 80 minutes, and then separate the straw residue and acid solution by suction filtration, collect the hydrolysate, and refrigerate in a refrigerator at 4°C for later use.

[0078] Add 25% ammonia water to adjust the pH value of the hydrolyzed supernatant to 10, filter out the precipitate with filter paper to obtain the treated hydrolysate, and then fine-tune the pH to 10. Then it was sealed with a membrane and placed in a 25°C water bath to react overnight. Finally, the pH of the hydrolysate was adjusted to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (5-10min at room temperature), filter out the activated carbon with filter paper to obtain the detoxified hydrolyzed liquid, and then fine-tune the pH value to 5.5 with dilute sulfuric acid...

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Abstract

The invention relates to a method for producing bacterial cellulose with wheat straws / spruces, comprising the following steps: (1) grinding the wheat straws or spruces, soaking the ground wheat straws or spruces in dilute sulfuric acid or hydrochloric acid to react at 90-240 DEG C, then separating the residues of the wheat straws or spruces from the acid hydrolysate through pump filtration and collecting the hydrolysate for later use; (2) detoxicating the hydrolysate; and (3) taking the detoxicated hydrolysate as the carbon source in the culture medium and adding the nitrogen source to prepare the culture medium and inoculating acetobacter aceti or gluconobacter oxydans into a shaker at 25-30 DEG C and 160-250r / min to be cultured or an incubator at 25-30 DEG C to undergo static culture, thus obtaining the bacterial cellulose. The carbon source in the culture medium is good in quality, low in price and suitable for industrial production.

Description

Technical field [0001] The invention belongs to the field of preparation of bacterial cellulose, and particularly relates to a method for producing bacterial cellulose from wheat straw / spruce. Background technique [0002] Bacterial Cellulose (BC), also known as microbial cellulose (Microbial Cellulose), is a biological material with broad application prospects. Compared with other higher plant celluloses in nature, it has many unique properties. Including high purity, high crystallinity, high degree of polymerization, high water holding capacity, high tensile strength, strong biological adaptability, etc. Therefore, the cellulose material has huge application prospects in the fields of artificial skin and blood vessels, medical dressings, adhesives, acoustic equipment vibration membranes, papermaking, textiles, composite membranes and the like. However, the high cost of bacterial cellulose culture medium and the low cellulose output and yield are the bottlenecks in its industri...

Claims

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Application Information

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IPC IPC(8): C12P19/04C12R1/02C12R1/01
Inventor 洪枫杨光杨雪霞
Owner DONGHUA UNIV
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