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Method for preparing bacterial cellulose by using bagasse

A technology of bacterial cellulose and bagasse, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problem that bagasse is not effectively used, and achieve the improvement of economic utilization value, large processing capacity, and raw material Effects from a wide range of sources

Active Publication Date: 2013-05-15
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Sugarcane is the main energy crop in southern my country, and the large amount of bagasse left after sugar extraction has not been effectively utilized.

Method used

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  • Method for preparing bacterial cellulose by using bagasse

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Effect test

Embodiment 1

[0034] The bagasse is first ground with a plant grinder, and then soaked in dilute sulfuric acid (3%, w / v) in the reactor at a solid / liquid ratio of 1:6 bagasse to dilute acid solution overnight (12-24h). Then react at a temperature of 180°C for 60 minutes, then separate the bagasse residue and the acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4°C for later use.

[0035]Add NaOH to adjust the pH value of the hydrolyzed liquid to about 10, filter the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 30°C water bath for 12-24 overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) activated carbon, stir (5-10min at room temperature) and filter out the activated carbon with filter paper to obtain a detoxified hydrolyzed liquid, then fine-tune the pH value to 5.0-5.5 with dilute sulfuri...

Embodiment 2

[0037] Bagasse is first ground with a plant grinder, and then soaked overnight (12-24h) in a reactor with dilute hydrochloric acid (1%, w / v) at a solid / liquid ratio of 1:10 bagasse to dilute acid solution, Then react at a temperature of 240°C for 75 minutes, then separate the bagasse residue and the acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4°C for later use.

[0038] Add Ca(OH) 2 Adjust the pH value of the hydrolyzed liquid to about 10, filter out the precipitate with filter paper to obtain the treated hydrolyzed liquid, and then fine-tune the pH value to 10.0. Then seal it with a film, place it in a 40°C water bath for 12-24 overnight, and finally adjust the pH value of the hydrolyzate to 5.0 with dilute acid. Then add 2% (mass percentage) of activated carbon, stir (5-10min at room temperature) and filter out the activated carbon with filter paper to obtain a detoxified hydrolyzed liquid, then fine-tune the pH value to 5.5 with dilute ...

Embodiment 3

[0040] Bagasse is first ground with a plant grinder, and then soaked overnight (12-24h) in a reactor with dilute sulfuric acid (2%, w / v) at a solid / liquid ratio of bagasse to dilute acid solution of 1:15, Then react at a temperature of 200°C for 80 minutes, then separate the bagasse residue and the acid solution by suction filtration, collect the hydrolyzate, and refrigerate at 4°C for later use.

[0041] First wash the anion exchange resin AG1-X8 with clean water for several times, and filter off the washing water for later use. Then add an ion exchange resin to the hydrolyzate in an amount of 3-10w / v% until the pH value is 10. Under the condition of room temperature or 50°C, shake at 90rpm for 30-60min. After the resin is removed by filtration or centrifugation, the treated hydrolyzate is obtained, and then the pH value is finely adjusted to 4-6. The detoxified hydrolyzate is tested for sugar and used as the carbon source of the medium, and then 0.1%-1% of yeast extract an...

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Abstract

The invention relates to a method for preparing bacterial cellulose by using bagasse. The method comprises steps of: (1) drying and crushing the bagasse, soaking the bagasse in acid with a solid-liquid ratio of 1:5-1:20, then reacting at 100 DEG C-240 DEG C for 50-90 min, filtering after the reaction, and collecting a hydrolysate; (2) detoxifying the hydrolysate; and (3) using the detoxified hydrolysate as a carbon source for a medium, adding 0.1-2% of a nitrogen source, and sterilizing at 121 DEG C for 15-20 min to obtain a culture medium; inoculating bacillus aceticus or Gluconobacter oxydans with an inoculation amount of 5%-10%; and conducting shaking culture at 25-30 DEG C with 160-250rpm or conducting static culture at 25-30 DEG C for 6-25 days, so as to obtain bacterial cellulose. The culture medium carbon source prepared by the invention has good quality and low price, and is suitable for industrialized production.

Description

technical field [0001] The invention belongs to the field of bacterial cellulose preparation, in particular to a method for preparing bacterial cellulose from bagasse. Background technique [0002] Bacterial Cellulose (BC for short), also known as Microbial Cellulose, is a biological material with broad application prospects. Compared with other higher plant cellulose in nature, it has many unique properties. Including high purity, high crystallinity, high degree of polymerization, high water holding capacity, high tensile strength, strong biological adaptability, etc. Therefore, the cellulose material has great application prospects in the fields of artificial skin and blood vessels, medical dressings, adhesives, vibration membranes for audio equipment, papermaking, textiles, and composite membranes. However, the high cost of bacterial cellulose culture medium, low cellulose yield and yield are the bottlenecks of its industrial production and popularization and application...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01C12R1/02
Inventor 陈琳洪枫
Owner DONGHUA UNIV
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