Method for preparing glucosamine and chitin simultaneously by using of shrimp and crab waste
A technology of glucosamine and chitin, applied in the field of simultaneous extraction of active substances glucosamine and chitin, can solve the problems of high cost, strong microbial reproduction ability, low cost, etc., achieve high content of active components, realize complete utilization, and reduce environment The effect of pollution
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Embodiment 1
[0021] Preparation of homogenate: Take 20 g coarse powder of fresh shrimp and crab heads and shrimp and crab shells, add 60 g of water to dissolve, and sterilize at 120°C for 25 minutes.
[0022] Add glucose: collect the homogenate and add 160 g of 10% glucose solution to homogenize for 3 minutes.
[0023] Introduced strains: (1) Activate Bacillus subtilis and Gluconobacter oxydans that have been refrigerated in sequence. Activation method: Inoculate in the seed medium and place in a 37°C incubator for 28 hours with shaking. Bacillus subtilis was first inoculated into the culture medium with an inoculation amount of 8%, cultured at 38°C for 48 hours, and then inoculated with Gluconobacter oxydans in the same way at 38°C for 24 hours. The desalination rate in the determination process is: 93.52%, and the deproteinization rate is 95.32%.
[0024] (2) Sterilize after the fermentation is complete, then add the symbiotic system of Streptococcus thermophilus, Lactobacillus acidoph...
Embodiment 2
[0029] Others are the same as in Example 1, except that the inoculation amounts of Bacillus subtilis and Gluconobacter oxydans are 5% respectively, and the desalination rate in the determination process is 91.32%, and the deproteinization rate is 90.63%.
Embodiment 3
[0031] Others are the same as in Example 1, except that the inoculum amounts of Bacillus subtilis and Gluconobacter oxydans are 6% respectively, and the desalination rate in the measurement process is 92.65%, and the deproteinization rate is 91.25%.
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