Method for preparing glucosamine and chitin simultaneously by using of shrimp and crab waste

A technology of glucosamine and chitin, applied in the field of simultaneous extraction of active substances glucosamine and chitin, can solve the problems of high cost, strong microbial reproduction ability, low cost, etc., achieve high content of active components, realize complete utilization, and reduce environment The effect of pollution

Inactive Publication Date: 2018-02-02
山东智宇知识产权运营中心有限公司莱州分公司
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

In the process of this patent, enzymes are used for many times in production, and the cost of enzyme preparations is expensive, which is not suitable for large-scale industrial production. The microbial reproduction ability is strong, the cost is low, and the yield is high. The advantages are obvious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Preparation of homogenate: Take 20 g coarse powder of fresh shrimp and crab heads and shrimp and crab shells, add 60 g of water to dissolve, and sterilize at 120°C for 25 minutes.

[0022] Add glucose: collect the homogenate and add 160 g of 10% glucose solution to homogenize for 3 minutes.

[0023] Introduced strains: (1) Activate Bacillus subtilis and Gluconobacter oxydans that have been refrigerated in sequence. Activation method: Inoculate in the seed medium and place in a 37°C incubator for 28 hours with shaking. Bacillus subtilis was first inoculated into the culture medium with an inoculation amount of 8%, cultured at 38°C for 48 hours, and then inoculated with Gluconobacter oxydans in the same way at 38°C for 24 hours. The desalination rate in the determination process is: 93.52%, and the deproteinization rate is 95.32%.

[0024] (2) Sterilize after the fermentation is complete, then add the symbiotic system of Streptococcus thermophilus, Lactobacillus acidoph...

Embodiment 2

[0029] Others are the same as in Example 1, except that the inoculation amounts of Bacillus subtilis and Gluconobacter oxydans are 5% respectively, and the desalination rate in the determination process is 91.32%, and the deproteinization rate is 90.63%.

Embodiment 3

[0031] Others are the same as in Example 1, except that the inoculum amounts of Bacillus subtilis and Gluconobacter oxydans are 6% respectively, and the desalination rate in the measurement process is 92.65%, and the deproteinization rate is 91.25%.

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Abstract

The invention relates to the reuse of shrimp and crab waste, specifically a method for simultaneously producing shrimp and crab xanthin and chitosan by using shrimp and crab waste, using the synergistic effect of Bacillus subtilis and Gluconobacter oxydans to add glucose to ferment Shrimp and crab skin and crab head are desalinated and deproteinized, and then fermented by Streptococcus thermophilus, Lactobacillus acidophilus, and Lactobacillus bulgaricus for one week to decalcify and decolorize, use chitin deacetylase to treat chitin, and use leather Glucosamine can be obtained by processing Plasmomonas Shigella, a lambs negative bacteria. During the fermentation process, there is no need to add a large amount of acid and alkali, which reduces environmental pollution, and microbial fermentation saves costs compared with enzyme fermentation, and the content of active ingredients obtained is higher, which realizes the complete utilization of active ingredients of raw materials, and is economically feasible.

Description

technical field [0001] The invention relates to the reuse of shrimp and crab waste, specifically a method for simultaneously producing glucosamine and chitin from shrimp and crab waste, that is, using microbial collaborative fermentation technology to simultaneously extract active substance glucosamine from shrimp and crab waste , Chitin method. Background technique [0002] The output of shrimp and crab in our country is very high and exported to foreign markets. During the eating and processing of fresh shrimp and crab, the head and shell are mainly discarded, and the lack of full utilization results in a great waste of nutrients in shrimp and crab shells and shrimp and crab heads. It also increases the burden on the environment. Shrimp and crab heads and shells are rich in chitin, amino acids, unsaturated fatty acids, minerals and various trace elements. At present, many countries in the world are focusing on developing this resource. [0003] Production of chitin and it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P19/26C12P19/04C12P19/02C12R1/125C12R1/01C12R1/46C12R1/23C12R1/225
CPCC12P39/00C12P19/02C12P19/04C12P19/26
Inventor 曲广旭
Owner 山东智宇知识产权运营中心有限公司莱州分公司
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