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Application of gluconobacter oxydans in preparing 1,3-dioxyacetone

A technology of Gluconobacter oxydans and oxidizing glucose, applied in the fields of fermentation engineering and enzyme engineering, can solve the problems of increasing process difficulty and cost, being unable to tolerate high-concentration glycerol, unfavorable for industrialized production, etc., and achieving the effect of facilitating separation and purification

Inactive Publication Date: 2011-01-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the above methods, the conversion rate of the production bacteria is low, and it cannot tolerate high concentration of glycerol, so other production methods are used to increase the yield, which increases the difficulty and cost of the process, and is not conducive to industrial production.

Method used

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  • Application of gluconobacter oxydans in preparing 1,3-dioxyacetone
  • Application of gluconobacter oxydans in preparing 1,3-dioxyacetone

Examples

Experimental program
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Embodiment 1

[0031] Incline medium: glucose 30g / L, yeast extract 5g / L, beef extract 3g / L, agar 20g / L.

[0032] Fermentation medium (same as shake flask medium): sorbitol 10g / L, glycerin 40g / L, yeast extract 5g / L, beef extract 3g / L, MgSO 4 ·7H 2 O 0.5g / L, CaCO 3 0.5g / L, the initial pH is 5.0, and sterilized at 121°C for 15min.

[0033] Cultivate Gluconobacter oxydans CGMCC No.2709 on the slant medium at 30°C for 24 hours, then use an inoculation loop to connect a ring of bacteria from the slant to the shake flask medium, cultivate at 30°C for 24 hours, and spin the shaker flask at 200r / min, centrifuge Collect the thalli in the fermentation broth, transform with free cells, weigh 1.5g of centrifuged wet cells and add them to 10mL of 100g / L glycerin solution (the amount of cells added is calculated by converting 1L of 100g / L glycerol solution per 150g of cells) , under the condition of 30° C., transform on a shaking table at 200 r / min for 24 h, and measure the conversion rate of the substr...

Embodiment 2

[0035] The medium composition is the same as in Example 1. Cultivate Gluconobacter oxydans CGMCC No.2709 on a slant medium at 30°C for 24 hours, and then use an inoculation loop to connect a ring of bacteria from the slant to the shake flask medium, cultivate at 28°C for 30 hours, and centrifuge at 120r / min. Collect the thalli in the fermentation broth, wash twice with pH7.0 potassium phosphate buffer, weigh 1.5 g of centrifuged wet cells and add them to 10 mL of 160 g / L glycerol solution (the amount of cells added is calculated as 1 L of 160 g / L per 150 g of cells). L of glycerol solution), under the condition of 28°C, convert and produce DHA in a shaking table at 120r / min, convert for 48h, measure the conversion rate of the substrate and the concentration of the product after the reaction finishes. The conversion rate of glycerol was 97%, and the concentration of DHA was 154g / L. Example 3:

Embodiment 3

[0036] The medium composition is the same as in Example 1. Cultivate Gluconobacter oxydans CGMCC No.2709 in the slant medium at 30°C for 24 hours, then use an inoculation loop to connect a ring of bacteria from the slant to the shake flask medium, cultivate at 32°C for 40 hours, and spin the shaker flask at 220r / min, centrifuge Collect the thalli in the fermentation broth, wash twice with pH7.0 potassium phosphate buffer, weigh 1.5 g of centrifuged wet cells and add them to 10 mL of 200 g / L glycerin solution (the amount of cells added is 1 L of 200 g / L per 150 g of cells) L of glycerol solution), under the condition of 30°C, convert and produce DHA in a shaking table at 200r / min, convert for 60h, measure the conversion rate of the substrate and the concentration of the product after the reaction finishes. The conversion rate of glycerol was 90%, and the yield of DHA was 166.2 g / L.

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Abstract

The invention discloses application of gluconobacter oxydans in preparing 1,3-dioxyacetone. The gluconobacter oxydans is named gluconobacter oxydans NH-10, and preserved in the China General Microbiological Culture Collection Center with the number of CGMCC No.2709 and the preservation data of October 14, 2008. The gluconobacter oxydans NH-10 can convert glycerol in high efficiency under a condition of high concentration glycerol to produce the 1,3-dioxyacetone. Under the technical condition of the application, the conversion rate of the glycerol can reach 99.7 percent (w / w), and the yield of the 1,3-dioxyacetone can reach 97 percent, the concentration of the 1,3-dioxyacetone in the conversion solution reaches 166.2g / L, and other components are scarcely contained in the conversion solution. The microorganism strain is applicable to industrial production of dioxyacetone.

Description

technical field [0001] The invention belongs to the technical fields of fermentation engineering and enzyme engineering, and relates to a glycerol dehydrogenase-producing microorganism Gluconobacter oxydans and a method for using it to produce dihydroxyacetone Background technique [0002] 1,3-Dihydroxyacetone, or dihydroxyacetone (1,3-dihydroxyaeetone or dihydroxyacetone, abbreviated as DHA) is the simplest three-carbon ketose sugar, which is a white powdery crystal with a sweet taste, easily soluble in Solvents such as water, ethanol, acetone, etc., contain two hydroxyl groups and one ketone group in their molecules, and are chemically active. They are important intermediates and raw materials for pharmaceuticals and chemical synthesis. They are used in various industries such as fine chemicals, food industry, and cosmetics industry. play an important role. [0003] There are two ways to produce DHA, chemical method and biological method. Compared with the chemical synthe...

Claims

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Application Information

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IPC IPC(8): C12P7/26C12N1/20C12R1/01
Inventor 徐虹戴小燕朱宏阳李莎冯小海
Owner NANJING UNIV OF TECH
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