Method of fementing shrimp heads to prepare active substances, chitin and organic acidity calcium

A technology for active substances and organic acid calcium, which is applied in the field of fermenting shrimp heads to prepare active substances, chitin and organic acid calcium, can solve the problems of high cost of enzyme preparations and limit the large-scale application of chitin, and achieves reduction of production costs and shrimp production. Strong fragrance and the effect of reducing environmental pollution

Active Publication Date: 2013-07-31
青岛新辰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the enzymatic hydrolysis method avoids the use of a large amount of strong alkali reagents, the cost of commercial enzyme preparations is very high, which also limits the large-scale application of chitin prepared from shrimp heads

Method used

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  • Method of fementing shrimp heads to prepare active substances, chitin and organic acidity calcium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take frozen prawn heads, weigh about 15g after thawing at room temperature, add a little water, homogenize through a high-shear homogenizer, and collect the homogenate. Add water 8 times the weight of the shrimp head to prepare the culture medium and sterilize at 121°C. Inoculate Bacillus licheniformis (B. licheniformis) that has been purified and refrigerated or vacuum freeze-dried into the seed medium (peptone 1%, yeast extract 0.5%, sodium chloride 1%, pH 7.2, the above concentrations are mass volume percentages ), placed in a 37°C incubator for 24 h with shaking, and then inoculated at 10% of the inoculation amount in the shrimp head culture medium for 48 h at 37°C, centrifuged at 4000 r / min for 5 min, and the fermentation broth and fermentation residue were collected respectively. The liquid part was spray-dried to obtain 1.09g of composite protein powder, and the precipitated part was dried to obtain 1.90g of chitin. The deproteinization rate and desalination rate...

Embodiment 2

[0023] Take the frozen prawn heads, weigh about 15g after thawing at room temperature, add a little water, homogenize through a high-shear homogenizer, and collect the homogenate. Add 10% glucose solution 8 times the weight of the shrimp head to prepare the medium and sterilize at 115°C. As for the added concentration of glucose, skilled artisans can adjust it according to the situation, and it is not limited to the description in this embodiment. Activate the purified Bacillus licheniformis stored in cold storage or vacuum freeze-drying. Other operations are the same as in Example 1. Finally, 1.20 g of protein and calcium gluconate composite powder and 1.69 g of chitin were obtained. The deproteinization rate and desalination rate in the determination process were 93.69% and 45.46%, respectively. It can be seen that the supplementation of additional carbon source (glucose) can improve the deproteinization rate of shrimp heads fermented by Bacillus licheniformis, and the des...

Embodiment 3

[0025] The pretreatment of the shrimp head is the same as in Example 1 (the aqueous glucose solution is not added to the slurry). Activate the purified Gluconobacter oxydans (G. oxydans) that has been refrigerated or vacuum freeze-dried. Other operations are the same as in Example 1. Finally, 1.53g of protein and calcium gluconate composite powder and 1.12g of chitin were obtained. The deproteinization rate and desalination rate in the determination process were 40.71% and 93.46%, respectively. The results show that Gluconobacter oxidans can grow with shrimp head as the only carbon and nitrogen source, can be directly fermented without sterilization, and has a high desalination rate, but the deproteinization rate is low, which is not as good as that of Bacillus licheniformis. / 2, as shown in Table 1.

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Abstract

The invention provides a method of fementing shrimp heads to prepare active substances, chitin and organic acidity calcium, wherein Bacillus licheniformis and Gluconobacter oxydans are utilized to ferment the shrimp heads to prepare the active substances, the chitin and the organic acidity calcium. The method utilizes synergistic effects of the Bacillus licheniformis and the Gluconobacter oxydans to ferment the shrimp head, is free of adding any acid and alkali, and meanwhile uses tap water to ferment, thereby greatly reducing production cost. The obtained broth is rich in shrimp incense, free of fishy smell and bitter taste, and simultaneously rich in amino acids, polyphenol, compound enzymes and a plurality of beneficial components, and can be used for producing flavourings, hydrolyzed protein products, functional foods, health products and other fields. By using the method provided by the invention, a plurality of active substances can be simultaneously separated and prepared from a batch of shrimp heads, such as hydrolyzed proteins rich in the amino acids, the polyphenol and a plurality of active substances; food-grade chitin; and calcium gluconate relatively appropriate for the body to absorb, thereby realizing comprehensive utilization of effective components in the raw materials.

Description

technical field [0001] The invention relates to the technical field of extracting active substances, chitin and calcium organic acid from shrimp heads by using a mixed microorganism collaborative fermentation technology. Background technique [0002] The output of prawns in our country is high, and the products are mainly exported, and the export products are mainly headless shrimp (shrimp). In the process of producing frozen shrimp, a large amount of waste such as shrimp heads and shrimp shells (accounting for 30-40% of the mass of prawns) will be produced, which are currently mainly used to produce feed, seasoning and chitin, and the rest are directly discarded . Shrimp processing by-products are rich in protein, chitin, amino acids, polyphenols, minerals, lipids and other components. Direct discarding not only wastes precious resources, but also pollutes the environment. The development approach of shrimp heads has been recognized by people because of its rich nutrition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P41/00C12P21/06C12P7/58C08B37/08C12R1/10C12R1/01
Inventor 毛相朝刘培林洪薛长湖
Owner 青岛新辰生物科技有限公司
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