Improved culture medium suitable for vegetative growth of nostoc sphaeroids kutz and preparation method

Abstract

The invention discloses an improved culture medium suitable for vegetative growth of nostoc sphaeroids kutz and a preparation method. The preparation method comprises the following steps: respectively preparing a solution A mother solution, a solution B mother solution, a solution C mother solution, a solution D mother solution, a solution E mother solution and a solution F mother solution;sequentially dissolving a certain amount of mother liquor of the solution A, the solution B, the solution C, the solution D, the solution E and the solution F into a solvent during use, adjusting the pH value to 7.1 by using sodium hydroxide or hydrochloric acid, and sterilizing for 20 minutes at 121 DEG C and 0.1 Mpa. The solution A is prepared from 25.0 g / L of sodium nitrate, 0.800 g / L of dipotassium phosphate and 1.50 g / L of magnesium sulfate heptahydrate; the solution B is prepared from 3.00 g / L of citric acid, 3.00 g / L of ferric ammonium citrate and 0.500 g / L of ethylenediamine tetraacetic acid disodium salt; the solution C is 13.6 g / L anhydrous calcium chloride; the solution D is 20.0 g / L sodium carbonate; the solution E is prepared from 2.86 g / L of boric acid, 1.81 g / L of manganese chloride tetrahydrate, 0.220 g / L of zinc sulfate heptahydrate, 0.390 g / L of sodium molybdate, 0.0800 g / L of copper sulfate pentahydrate and 0.0400 g / L of cobalt chloride hexahydrate; the solution F is a soil leaching solution preparedby adding 1000mL of clean tap water into 1000g of soil, stirring, and filtering with filter paper; and the solvent is distilled water. The improved culture medium can be stored.

Description

technical field [0001] The present invention relates to a culture medium and preparation method suitable for microalgae cultivation, in particular to an improved culture medium and preparation method suitable for the growth of kudzu rice, in particular to an improved culture medium suitable for vegetative growth of kudzu rice And the preparation method, the culture medium is suitable for the large-scale cultivation of common kudzu and emerald green kudzu. Background technique [0002] Ge Xianmi ( Nostoc sphaeroides Kützing), commonly known as Tian Agaric and Tianxian rice, is a multicellular group of freshwater cyanobacteria, which belongs to Cyanophyta, Cyanophyceae, Hormogonales, and Nostocaceae in taxonomy. , Nostoc ( Nostoc) . Gexianmi is a kind of precious wild edible and medicinal dual-purpose cyanobacteria traditionally exported in my country, which has rich nutritional value. According to modern nutritional testing and analysis, Gexianmi is rich in protein, vita...

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a kind of improved culture medium and preparation method suitable for the vegetative growth of Gexianmi in view of the deficiencies in the prior art, and improve the traditional BG11 culture medium, which can effectively solve the problem of traditional culture medium Easy to form precipitation leading to nutrient deficiency, unable to store, cumbersome to use, high price of raw materials and other technical defects

Images