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Method for improving tribonema biomass and metabolites by using magnetic field intervention

A technology of yellow hair algae and biomass, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, can solve the problems of large demand gap for microalgae biomass, and achieve considerable application and Promotional value, improved biomass accumulation, and low-cost effects

Active Publication Date: 2019-03-29
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature reports, the total production of microalgae biomass in my country is only about 10,000 tons / year, but only preliminary statistics show that the amount of bait algae required by seawater fish, prawns and bivalve molluscs can reach 16,000 tons, causing microalgae biomass The gap in demand is huge

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Pour the prepared BG11 medium into the column reactor, insert the logarithmic phase of Tribonema sp. (China Freshwater Algae Germplasm Bank), the inoculation amount is 10% of the volume of the medium, and place it in the climate In the incubator, set the temperature at 25°C, 24h continuous light, the light intensity is 1500Lx, and the 2 air, CO 2 It is 1.5% of the air volume, and the ventilation rate is 0.5vvm; after culturing for 1d, apply an additional 100mT static magnetic field strength for 24h, and after continuing to cultivate for 7d, carry out the detection of biomass and oil content;

[0025] The biomass treated with a magnetic field can reach 6.0g / L, while the blank control group without a magnetic field is only 3.7g / L; meanwhile, the oil content of the treated with a magnetic field is 60% of the dry weight of Xanthophyllum, and the control group without a magnetic field The lipid content of the blank control group was 42% of the dry weight of Xanthophyllum, w...

Embodiment 2

[0027] Pour the prepared fresh BG11 medium into the light column reactor, insert the logarithmic phase of the yellow hair algae Tribonemasp. (preserved by the School of Food Science, Jiangsu University), the inoculation amount is 10% of the medium volume, and place it in the climate incubator , set the temperature at 25°C, 24 hours of continuous light, light intensity of 1500Lx, direct access to air, ventilation rate of 0.5vvm; after 1 day of culture, apply 60mT static magnetic field strength for continuous treatment throughout the growth period, after 7 days of culture, carry out biological Detection of fat and fat content;

[0028] The biomass treated with magnetic field can reach 4.7g / L, while the blank control group without magnetic field is only 2.5g / L. At the same time, the carbohydrate content is 50% of the dry weight of the yellow hair algae, and the carbohydrate content of the blank control group without a magnetic field is 36% of the dry weight of the yellow hair alg...

Embodiment 3

[0030] Pour the prepared BG11 medium into the column reactor, insert the logarithmic phase of Tribonema sp. (China Freshwater Algae Germplasm Bank), the inoculation amount is 5% of the medium volume, and place in the climate In the incubator, the temperature is set at 30°C, the light is set to a 12h / 12h light-dark cycle, the light intensity is 5000Lx, and a mixture of CO 2 air, CO 2 It is 10% of the air volume, and the ventilation rate is 0.2vvm; after culturing for 1d, apply a static magnetic field strength of 10mT to treat for 24h, and after continuing to cultivate for 7d, carry out the detection of biomass and oil content;

[0031] The biomass treated with a magnetic field can reach 4.1g / L, while the blank control group without a magnetic field is only 2.3g / L; at the same time, the oil content of the treated with a magnetic field is 53% of the dry weight of Xanthophyllum, and the The lipid content of the blank control group was 37% of the dry weight of Xanthophyllum, which...

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Abstract

The invention belongs to the technical field of aquatic product processing or biophysical processing, and relates to a method for improving tribonema biomass and metabolites by using magnetic field intervention. The specific steps are as follows: firstly, preparing a BG11 culture medium, pouring the culture medium into a column reactor, and inoculating the culture medium with tribonema sp. in logarithmic phase; placing the reactor in a climate incubator, setting a certain temperature, illumination intensity and illumination time, introducing a certain amount of air containing CO2, and carryingout culturing; and after 1 day of culturing, applying additional magnetic field treatment, continuing the culturing, and detecting biomass and oil accumulation after 7 days of culturing. The method can increase the content of lipids, saccharides, proteins or natural metabolites with natural biological activity in the cells by adding a magnetic field to a culture system of filamentous microalgae such as the tribonema, and can fully exert the economic potential of the filamentous microalgae.

Description

technical field [0001] The invention belongs to the technical field of aquatic product processing or biophysical processing, and specifically relates to a method for increasing the biomass and metabolites of Xanthophyllum algae by using magnetic field intervention. Background technique [0002] Microalgae has very broad application potential in many fields, and it can be used as raw materials for the production of biofuels, food and animal feed additives, and biologically active substances. According to literature reports, the total production of microalgae biomass in my country is only about 10,000 tons / year, but only preliminary statistics show that the amount of bait algae required by seawater fish, prawns and bivalve molluscs can reach 16,000 tons, causing microalgae biomass The gap in demand is huge. [0003] The cost of harvesting single-cell microalgae can account for 20-30% of the total cost of microalgae production; the equipment investment is close to 90% of the ov...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N13/00C12N1/12C12P7/64C12P19/00C12R1/89
CPCC12N1/12C12N13/00C12P7/6463C12P19/00
Inventor 霍书豪陈秀钱静亚朱菲菲张存胜邹彬
Owner JIANGSU UNIV
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