A method of increasing the biomass and metabolites of Zanthrocystis algae by using magnetic field intervention
A technology of yellow hair algae and biomass, applied in the direction of microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, can solve the problems of large demand gap for microalgae biomass, and achieve considerable application and Promotional value, wide application range, and the effect of increasing growth rate
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Embodiment 1
[0024] The prepared BG11 medium was poured into the column reactor, and the logarithmic phase of Tribonemasp. (Chinese freshwater algae gene bank) was inserted, and the inoculation amount was 10% of the medium volume. In the incubator, set the temperature to 25°C, continuously illuminate for 24h, the light intensity is 1500Lx, and the mixture is mixed with CO. 2 air, CO 2 It is 1.5% of the air volume, and the ventilation volume is 0.5vvm; after culturing for 1 day, apply a static magnetic field strength of 100 mT for 24 hours, and continue to culture for 7 days, and then detect the biomass and oil content;
[0025] The biomass treated with magnetic field can reach 6.0g / L, while the blank control group without magnetic field is only 3.7g / L; meanwhile, the oil content of treated with magnetic field is 60% of the dry weight of Xanthomonas algae. The lipid content of the blank control group was 42% of the dry weight of Xanthomonas, indicating that the external magnetic field had ...
Embodiment 2
[0027] Pour the prepared fresh BG11 medium into the light column reactor, insert the logarithmic phase of the yellow alga Tribonemasp. (preserved by the School of Food Science, Jiangsu University), the inoculation amount is 10% of the medium volume, and place it in a climate incubator , set the temperature at 25°C, continuously illuminated for 24h, the light intensity is 1500Lx, the air is directly introduced, and the ventilation volume is 0.5vvm; after culturing for 1d, the continuous treatment with 60mT static magnetic field intensity is applied throughout the growth period, and after culturing for 7d, the biological Quantity and oil content detection;
[0028] The biomass treated with magnetic field can reach 4.7g / L, while the blank control group without magnetic field is only 2.5g / L. At the same time, the carbohydrate content was 50% of the dry weight of Xanthomonas, and the carbohydrate content of the blank control group without the magnetic field was 36% of the dry weigh...
Embodiment 3
[0030] The prepared BG11 medium was poured into the column reactor, and the logarithmic phase of Tribonemasp. (Chinese freshwater algae gene bank) was inserted, and the inoculation amount was 5% of the medium volume. In the incubator, set the temperature to 30 °C, set the light to a 12h / 12h light-dark cycle, and set the light intensity to 5000Lx. 2 air, CO 2 It is 10% of the air volume, and the ventilation volume is 0.2 vvm; after culturing for 1 day, apply a static magnetic field strength of 10 mT for 24 hours, and continue to culture for 7 days, and then detect the biomass and oil content;
[0031] The biomass treated with magnetic field can reach 4.1g / L, while the blank control group without magnetic field is only 2.3g / L. The lipid content of the blank control group was 37% of the dry weight of Xanthomonas, indicating that the external magnetic field could promote the biomass and oil accumulation of Xanthomonas.
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