Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method of increasing the biomass and metabolites of Zanthrocystis algae by using magnetic field intervention

A technology of yellow hair algae and biomass, applied in the direction of microorganism-based methods, biochemical equipment and methods, and treatment of microorganisms with electricity/wave energy, can solve the problems of large demand gap for microalgae biomass, and achieve considerable application and Promotional value, wide application range, and the effect of increasing growth rate

Active Publication Date: 2022-06-21
JIANGSU UNIV
View PDF14 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to literature reports, the total production of microalgae biomass in my country is only about 10,000 tons / year, but only preliminary statistics show that the amount of bait algae required by seawater fish, prawns and bivalve molluscs can reach 16,000 tons, causing microalgae biomass The gap in demand is huge

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The prepared BG11 medium was poured into the column reactor, and the logarithmic phase of Tribonemasp. (Chinese freshwater algae gene bank) was inserted, and the inoculation amount was 10% of the medium volume. In the incubator, set the temperature to 25°C, continuously illuminate for 24h, the light intensity is 1500Lx, and the mixture is mixed with CO. 2 air, CO 2 It is 1.5% of the air volume, and the ventilation volume is 0.5vvm; after culturing for 1 day, apply a static magnetic field strength of 100 mT for 24 hours, and continue to culture for 7 days, and then detect the biomass and oil content;

[0025] The biomass treated with magnetic field can reach 6.0g / L, while the blank control group without magnetic field is only 3.7g / L; meanwhile, the oil content of treated with magnetic field is 60% of the dry weight of Xanthomonas algae. The lipid content of the blank control group was 42% of the dry weight of Xanthomonas, indicating that the external magnetic field had ...

Embodiment 2

[0027] Pour the prepared fresh BG11 medium into the light column reactor, insert the logarithmic phase of the yellow alga Tribonemasp. (preserved by the School of Food Science, Jiangsu University), the inoculation amount is 10% of the medium volume, and place it in a climate incubator , set the temperature at 25°C, continuously illuminated for 24h, the light intensity is 1500Lx, the air is directly introduced, and the ventilation volume is 0.5vvm; after culturing for 1d, the continuous treatment with 60mT static magnetic field intensity is applied throughout the growth period, and after culturing for 7d, the biological Quantity and oil content detection;

[0028] The biomass treated with magnetic field can reach 4.7g / L, while the blank control group without magnetic field is only 2.5g / L. At the same time, the carbohydrate content was 50% of the dry weight of Xanthomonas, and the carbohydrate content of the blank control group without the magnetic field was 36% of the dry weigh...

Embodiment 3

[0030] The prepared BG11 medium was poured into the column reactor, and the logarithmic phase of Tribonemasp. (Chinese freshwater algae gene bank) was inserted, and the inoculation amount was 5% of the medium volume. In the incubator, set the temperature to 30 °C, set the light to a 12h / 12h light-dark cycle, and set the light intensity to 5000Lx. 2 air, CO 2 It is 10% of the air volume, and the ventilation volume is 0.2 vvm; after culturing for 1 day, apply a static magnetic field strength of 10 mT for 24 hours, and continue to culture for 7 days, and then detect the biomass and oil content;

[0031] The biomass treated with magnetic field can reach 4.1g / L, while the blank control group without magnetic field is only 2.3g / L. The lipid content of the blank control group was 37% of the dry weight of Xanthomonas, indicating that the external magnetic field could promote the biomass and oil accumulation of Xanthomonas.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
pore sizeaaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention belongs to the technical field of aquatic product processing or biophysical processing, and relates to a method for increasing the biomass and metabolites of Xanthophyllum algae by using magnetic field intervention; the specific steps are as follows: first configure BG11 medium, pour it into a column reactor, and inoculate Yellow hair algae in the logarithmic phase ( Tribonema sp.); put it in the climate incubator, set a certain temperature, light intensity and light time, and pass a certain amount of CO mixed with 2 After cultivating for 1 day, apply an external magnetic field to continue cultivating, and detect biomass and oil accumulation after cultivating for 7 days; the present invention can increase its microalgae by adding a magnetic field to the culturing system of filamentous microalgae such as Xanthophylla The content of lipids, sugars, proteins or secondary metabolites with natural biological activity in the cells can give full play to the economic potential of the filamentous microalgae.

Description

technical field [0001] The invention belongs to the technical field of aquatic product processing or biophysical processing, and in particular relates to a method for increasing the biomass and metabolites of yellow silk algae by using magnetic field intervention. Background technique [0002] Microalgae have very broad application potential in many fields, and can be used as raw materials for the production of biofuels, food and animal feed additives, and biologically active substances. According to literature reports, the total output of microalgae biomass in my country is only about 10,000 tons per year. However, only preliminary statistics of the bait algae required for marine fish, prawns and bivalves can reach 16,000 tons, resulting in microalgal biomass. There is a huge gap in demand. [0003] The harvesting cost of single-celled microalgae can account for 20-30% of the total cost of microalgae production; the equipment input is close to 90% of the total equipment inp...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N13/00C12N1/12C12P7/6463C12P19/00C12R1/89
CPCC12N1/12C12N13/00C12P7/6463C12P19/00
Inventor 霍书豪陈秀钱静亚朱菲菲张存胜邹彬
Owner JIANGSU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products