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An endophytic fungus of the genus Phoma and its application

A technology of endophytic fungi and Phoma, applied in the fields of application, fungi, microorganisms, etc., can solve problems such as unpublished research reports, and achieve the effects of improving resistance, increasing biomass accumulation, and delaying symptoms of salt damage

Inactive Publication Date: 2017-04-12
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have also been research reports on the use of endophytic fungi to improve the salt resistance of soybeans, but no research report has been published on the role of endophytic fungi in improving the salt tolerance and salt resistance of rice and wheat

Method used

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  • An endophytic fungus of the genus Phoma and its application
  • An endophytic fungus of the genus Phoma and its application
  • An endophytic fungus of the genus Phoma and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Isolation, purification and identification of strain KDZ-5

[0032] 1.1 Isolation and purification of strain KDZ-5

[0033] 1.1.1 Medium Preparation

[0034] ①Potato dextrose agar (PDA): Potato (peeled) 200g, glucose 20g, agar 20g, add distilled water and make up to 1000mL; bacteria 20min).

[0035] ②Water agar medium (WA): 15 g of agar, add tap water to dilute to 1000 mL; then carry out conventional high temperature sterilization (1.1 atmospheres, sterilization at 121° C. for 20 min).

[0036] 1.1.2 Strain isolation and purification

[0037] The KDZ-5 strain of the present invention is obtained in the laboratory under the following isolation and culture conditions, including:

[0038]Sophora japonica plant samples collected from the saline-alkali land in Aksu, Xinjiang, China, the plant samples were kept fresh and brought back to the laboratory. The plant samples were rinsed with tap water to remove the soil and appendages on the surface; after drying at ...

Embodiment 2

[0079] Example 2 Experiment on the effect of KDZ-5 on improving the salt tolerance of rice seedlings

[0080] 2.1 Medium preparation

[0081] ① Potato dextrose agar (PDA): the same as in Example 1.

[0082] ②MS medium: potassium nitrate 1900mg / L, ammonium nitrate 1650mg / L, magnesium sulfate 370mg / L, potassium dihydrogen phosphate 170mg / L, calcium chloride 440mg / L, manganese sulfate 22.3mg / L, zinc sulfate 8.6mg / L , boric acid 6.2mg / L, potassium iodide 0.83mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025mg / L, ferrous sulfate 27.8mg / L, Na 2 EDTA37.3mg / L, glycine 2.0mg / L, thiamine hydrochloride 0.1mg / L, pyridoxine hydrochloride 0.5mg / L, niacin 0.5mg / L, inositol 100mg / L, 30g sucrose, agar powder 7g, H 2 Make up to 1L with O; pH 5.6-5.8.

[0083] 2.2 Culture of strain KDZ-5

[0084] Pick the mycelial block of strain KDZ-5 with a sterile toothpick and place it on a PDA medium plate, seal it with a parafilm and place it in a constant temperature bi...

Embodiment 3

[0097] Example 3 Hydroponic test of KDZ-5 improving salt tolerance of wheat seedlings

[0098] 3.1 Medium and solution preparation

[0099] ① Potato dextrose agar (PDA): the same as in Example 1.

[0100] ② Potato dextrose liquid medium (potato dextrose liquid, PBA): PDA medium without agar.

[0101] ③Hoagland nutrient solution: calcium nitrate tetrahydrate 945mg / L, potassium nitrate 506mg / L, ammonium nitrate 80mg / L, potassium dihydrogen phosphate 136mg / L, magnesium sulfate 493mg / L, iron salt solution 2.5mL, trace elements solution 5mL, pH=6.0.

[0102] ④Iron salt solution: 2.78 g of ferrous sulfate heptahydrate, 3.73 g of disodium EDTA, 500 mL of distilled water, pH=5.5.

[0103] ⑤ Trace element solution: potassium iodide 0.83mg / L, boric acid 6.2mg / L, manganese sulfate 22.3mg / L, zinc sulfate 8.6mg / L, sodium molybdate 0.25mg / L, copper sulfate 0.025mg / L, cobalt chloride 0.025 mg / L.

[0104] 3.2 Preparation of wheat seedlings

[0105] After surface disinfection (70% ethano...

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Abstract

The invention discloses an endophytic fungus of the genus Phoma and its application. The Phoma sp. endophytic fungus is named as Phoma sp. KDZ-5, and the preservation number is CCTCC No.M 2014162. The application is the application of the endophytic fungus of the Phoma genus in improving the salt tolerance of rice or wheat. The present invention finds for the first time that Phoma sp. KDZ-5 can improve the resistance ability of rice and wheat to salt stress. The test shows that after establishing a co-cultivation system with KDZ-5 strain and rice seedlings, the KDZ-5 strain can significantly Promote the growth of rice seedlings on salt-added MS plates; after establishing a co-culture system with wheat seedlings under nutrient solution hydroponic and soil pot conditions, KDZ-5 strains can significantly slow down or delay the salinity of wheat in saline-alkali soil damage symptoms, while greatly increasing the biomass accumulation of wheat.

Description

technical field [0001] The invention belongs to the field of microbial germplasm resource utilization, and in particular relates to an endophytic fungus belonging to the genus P. Background technique [0002] Soil salinization reduces the productivity of cultivated land and is a very important abiotic stress in food production, causing serious losses to the world's food production every year and seriously affecting the ecological balance of the region. Currently, more than 800 million hectares of land in the world suffer from land salinization, accounting for 6% of all land areas. Especially in regions with arid, semi-arid and Mediterranean-style climates, the problem is exacerbated. In recent years, unreasonable farming and irrigation, as well as environmental pollution and destruction of ecological balance are the main reasons for the problem of salinization. The problem of soil salinization in my country is very serious. The saline-alkali land has a wide distribution ra...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C09K17/00C12R1/645C09K101/00
CPCC09K17/00C09K2101/00C12N1/145C12R2001/645
Inventor 王洪凯陈亚平林福呈
Owner ZHEJIANG UNIV
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