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Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling

A tissue culture seedling and seedling refining technology, which is applied in the field of Tainong No. 16 pineapple tissue culture seedling refining and false planting, and achieves the effects of remarkable economic and social benefits, fast growth, and reduced difficulty and cost.

Inactive Publication Date: 2015-03-04
TROPICAL FRUIT RES INST HAINAN ACADOF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tissue culture seedling hardening and field transplanting technology have become the technical bottleneck restricting the promotion of virus-free tissue culture seedlings of Youxin pineapple variety. At present, there are still no relevant reports and production examples on the transplanting method of Tainong No. 16 pineapple tissue culture seedlings

Method used

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  • Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of Tainong No. 16 pineapple tissue culture seedling hardening and false planting method, is characterized in that, comprises the following steps:

[0035] (1) rooting culture of tissue culture seedlings

[0036] Put 20 ml of rooting medium into the tissue culture bag, after high temperature and humid heat sterilization, insert 8 tissue culture seedlings into each bag, and cultivate them in the tissue culture room to promote their rooting; the growth and development of the tissue culture seedlings are normal and grow to the When the height is ≥13 cm, the number of leaves is ≥8, the average leaf length is ≥12 cm, the number of fibrous roots is ≥10, and the average root length is ≥5 cm, prepare for hardening;

[0037] (2) seedling hardening

[0038] The tissue culture seedlings in the tissue culture bag described in step (1) are moved to the sunshade net shed by the tissue culture room, with 30 order insect-proof nets around the sunshade net shed; the shading rate ...

Embodiment 2

[0049] A kind of Tainong No. 16 pineapple tissue culture seedling hardening and false planting method, is characterized in that, comprises the following steps:

[0050] (1) rooting culture of tissue culture seedlings

[0051] Put 23 ml of rooting medium into the tissue culture bag, after high temperature and humid heat sterilization, insert 9 tissue culture seedlings into each bag of tissue culture bag, and cultivate them in the tissue culture room to promote their rooting; When the height is ≥13 cm, the number of leaves is ≥8, the average leaf length is ≥12 cm, the number of fibrous roots is ≥10, and the average root length is ≥5 cm, prepare for hardening;

[0052] (2) seedling hardening

[0053] The tissue culture seedlings in the tissue culture bag described in step (1) are moved to the sunshade net shed by the tissue culture room, with 40 order insect-proof nets around the sunshade net shed; the shading rate of the sunshade net shed is 90-95%, and the tissue culture bag i...

Embodiment 3

[0064] A kind of Tainong No. 16 pineapple tissue culture seedling hardening and false planting method, is characterized in that, comprises the following steps:

[0065] (1) rooting culture of tissue culture seedlings

[0066] Put 25 ml of rooting medium into the tissue culture bag, after high temperature and humid heat sterilization, insert 10 tissue culture seedlings into each bag of tissue culture bag, and cultivate them in the tissue culture room to promote their rooting; the growth and development of the tissue culture seedlings are normal and grow to the When the height is ≥13 cm, the number of leaves is ≥8, the average leaf length is ≥12 cm, the number of fibrous roots is ≥10, and the average root length is ≥5 cm, prepare for hardening;

[0067] (2) seedling hardening

[0068] The tissue culture seedling in the tissue culture bag described in step (1) is moved to the sunshade net shed by the tissue culture room, with 50 order insect-proof nets around the sunshade net sh...

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Abstract

The invention relates to the field of pineapple tissue culture, and discloses a hardening-seedling and temporary planting method for a Tainong No.16 pineapple tissue culture seedling. The method comprises the following steps: (1) rooting culture of a tissue culture seedling; (2) hardening-seedling; (3) preparation before transplanting; (4) transplanting; (5) management at transplanting time; (6) field planting. According to the hardening-seedling and temporary planting method for the Tainong No.16 pineapple tissue culture seedling provided by the invention, the transplantation survival rate of the pineapple tissue culture seedling can reach over 95%; the pineapple tissue culture seedling can quickly restore to growth after being transplanted; compared with other transplanting methods, the seedling time can be shortened by about 20 days; compared with a traditional seedling method, the seedling cost is lowered by over 20%; the plant grows and develops regularly evenly in the whole growth period; field management is facilitated; the fruits are good in economic characters, and have significant economic benefits and social benefits.

Description

technical field [0001] The invention relates to a method for hardening and false planting of Tainong No. 16 pineapple tissue cultured seedlings, and belongs to the technical field of pineapple tissue culture. Background technique [0002] Pineapple is one of the "Three Tropical Herbal Fruit Trees" in the world and one of the "Four Famous Lingnan Fruits" in my country. It is deeply loved by consumers because of its rich nutrition, sweet taste, and unique flavor. By 2011, the production area and output of pineapple in my country (excluding Taiwan Province) were 57,300 hectares and 1,519,100 tons, respectively, ranking among the top ten in the world. In the hot areas of our country, pineapple has always been one of the important sources of income for fruit farmers, and has extremely high economic and social importance. There are many varieties of pineapples, among which Tainong No. 16 is an excellent fresh pineapple variety bred by the Chiayi Agricultural Experiment in Taiwan....

Claims

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Application Information

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IPC IPC(8): A01G31/00A01G1/00
CPCA01G22/00A01G24/00
Inventor 何凡范鸿雁华敏王祥和胡福初罗志文何舒郭利军
Owner TROPICAL FRUIT RES INST HAINAN ACADOF AGRI SCI
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