Hardening-seedling and temporary planting method for Tainong No.16 pineapple tissue culture seedling
A tissue culture seedling and seedling refining technology, which is applied in the field of Tainong No. 16 pineapple tissue culture seedling refining and false planting, and achieves the effects of remarkable economic and social benefits, fast growth, and reduced difficulty and cost.
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[0033] Example 1
[0034] A method for refining and fake planting of Tainong No. 16 pineapple tissue culture seedlings is characterized in that it comprises the following steps:
[0035] (1) Tissue culture seedling rooting culture
[0036] The tissue culture bag is filled with 20 ml of rooting medium, and after sterilization by high temperature, humidity and heat, each bag of tissue culture bag is connected to 8 tissue culture seedlings, and the tissue culture room is cultured to promote rooting; the tissue culture seedlings grow normally and grow to plants When the height is ≥13 cm, the number of leaves is ≥8, the average leaf length is ≥12 cm, the number of fibrous roots is ≥10, and the average root length is ≥5 cm, prepare seedlings;
[0037] (2) Refining
[0038] Move the tissue cultured seedlings in the tissue culture bag described in step (1) from the tissue culture room to the sunshade net shed. The sunshade net shed is surrounded by 30 mesh insect-proof nets; the shading rate o...
Example Embodiment
[0048] Example 2
[0049] A method for refining and fake planting of pineapple tissue culture seedlings of Tainong No. 16 is characterized by comprising the following steps:
[0050] (1) Tissue culture seedling rooting culture
[0051] The tissue culture bag is filled with 23 ml of rooting medium. After sterilization by high temperature, humidity and heat, each bag of tissue culture bag is connected to 9 tissue culture seedlings, and the tissue culture room is cultivated to promote rooting; the tissue culture seedlings grow normally and grow to plants When the height is ≥13 cm, the number of leaves is ≥8, the average leaf length is ≥12 cm, the number of fibrous roots is ≥10, and the average root length is ≥5 cm, prepare seedlings;
[0052] (2) Seedling refinement
[0053] Move the tissue cultured seedlings in the tissue culture bag described in step (1) from the tissue culture room to the shade net shed. The shade net shed is surrounded by 40 mesh insect-proof nets; the shading rate of...
Example Embodiment
[0063] Example 3
[0064] A method for refining and fake planting of Tainong No. 16 pineapple tissue culture seedlings is characterized in that it comprises the following steps:
[0065] (1) Tissue culture seedling rooting culture
[0066] The tissue culture bag is filled with 25 ml of rooting medium. After sterilization by high temperature, humidity and heat, each bag of tissue culture bag is connected to 10 tissue culture seedlings. The tissue culture room is cultivated to promote rooting; the tissue culture seedlings grow normally and grow to plants When the height is ≥13 cm, the number of leaves is ≥8, the average leaf length is ≥12 cm, the number of fibrous roots is ≥10, and the average root length is ≥5 cm, prepare seedlings;
[0067] (2) Seedling refinement
[0068] Move the tissue cultured seedlings in the tissue culture bag described in step (1) from the tissue culture room to the shade net shed. The shade net shed is surrounded by 50 mesh insect-proof nets; the shading rate o...
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