Hordeum brevisubulatum indoor quick cultivation method

A cultivation method and barley technology, applied in the field of rapid indoor cultivation of wild barley, can solve the problems of low seed germination rate, slow growth, weak plants, etc., and achieve the effects of eliminating salt tolerance and drought resistance, vigorous growth, and tidy seedling emergence.

Inactive Publication Date: 2015-08-05
LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, because it belongs to wild germplasm, the biggest challenge when cultivating it indoors is low seed germination rate, slow growth, and weak plants; if no special treatment is done, its germination rate can only reach 30-50% under normal conditions. It takes 40-50 days to grow to the 4-leaf stage, and the leaves are small and yellow, which is not conducive to scientific research as

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  • Hordeum brevisubulatum indoor quick cultivation method
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  • Hordeum brevisubulatum indoor quick cultivation method

Examples

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Example Embodiment

[0019] Example 1:

[0020] A method for indoor rapid cultivation of wild barley includes the following steps:

[0021] 1) Remove the seeds of wild barley, select uniform seeds and treat them at -20℃ for 48h. After washing the wild barley seeds with distilled water, use 100mg / L gibberellin to germinate the seeds at a low temperature of 0-4℃ Pretreatment

[0022] 2) The pre-treated wild barley seeds obtained in step 1) are simply rinsed with distilled water, and then evenly sprinkled on the seed rack of the seed germination box for germination. The nutrient solution in the germination box is a nutrient solution containing 5mmol / L NaCl; for germination The indoor conditions are 24 / 18℃, dark, and wild barley seeds can germinate after 3 to 5 days, and the germination rate can reach 82%;

[0023] 3) Change the seedlings obtained in step 2) to light culture, 25℃, 16h light and 16℃, 8h without light, alternately, the light intensity is 600μmol / (m 2 ·S), the culture medium is hydroponics, an...

Example Embodiment

[0027] Example 2:

[0028] An indoor rapid cultivation method for wild barley, the steps are the same as in Example 1, except that the relevant temperature and NaCl concentration are adjusted, and it mainly includes the following steps:

[0029] 1) Remove the seeds of wild barley, select neat seeds and treat them at -18℃ for 48 hours. After washing the wild barley seeds with distilled water, use 150mg / L of gibberellin for seed germination at a low temperature of 0-4℃ Pretreatment

[0030] 2) The wild barley seeds obtained in step 1) are rinsed with distilled water, and then evenly sprinkled on the seed rack of the seed germination box for germination. The nutrient solution in the germination box is a nutrient solution containing 10mmol / L NaCl; for germination The indoor conditions are 26℃16h / 16℃8h, dark, the wild barley seeds can germinate after 3 to 5 days, and the germination rate can reach 85%;

[0031] 3) Change the seedlings obtained in step 2) to light culture, 24℃, 16h light ...

Example Embodiment

[0035] Example 3:

[0036] An indoor rapid cultivation method for wild barley, the steps are the same as in Example 2, except that the relevant temperature, time and NaCl concentration are adjusted, and it mainly includes the following steps:

[0037] 1) Remove the seeds of wild barley, select neat seeds and treat them at -16℃ for 24 hours. After washing the wild barley seeds with distilled water, use 200mg / L of gibberellin for seed germination at a low temperature of 0-4℃ Pretreatment

[0038] 2) The wild barley seeds obtained in step 1) are rinsed briefly with distilled water, and then evenly sprinkled on the seed rack of the seed germination box for germination. The nutrient solution in the germination box is a nutrient solution containing 15mmol / L NaCl; for germination The indoor conditions are 20℃16h / 16℃8h, dark, the wild barley seeds can germinate after 3 to 5 days, and the germination rate can reach 80%;

[0039] 3) Change the seedlings obtained in step 2) to light culture, ...

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Abstract

The invention discloses a hordeum brevisubulatum indoor quick cultivation method. The hordeum brevisubulatum indoor quick cultivation method comprises performing pre-germination treatment on low-temperature-treated hordeum brevisubulatum seeds through gibberellin, washing and then germinating in a nutrient solution containing NaCl, and performing light culture on germinated seedlings inside a hydroponic culture medium; after the germinated seedlings grow for 14-20 days, transplanting uniform seedlings to an earth culture environment; irrigating the seedlings with a doubled nutrient solution containing NaCl, and performing light culture on the seedlings. The hordeum brevisubulatum indoor quick cultivation method has the advantage of solving the problem of low germinating speed, weak plant and slow growth of the wild forage of hordeum brevisubulatum indoors, being capable of rapidly breaking dormancy of the hordeum brevisubulatum seeds and achieving a germination rate of higher than 80% within 3-5 days, obtaining uniform germination, and ensuring that the seedlings grow vigorously in the nutrient solution containing NaCl. The hordeum brevisubulatum indoor quick cultivation method is significant to the aspects such as indoor study of poor biological repeatability of biological characteristics and salt-enduring and drought-resistant performance of the hordeum brevisubulatum to eliminating poor plant growth and low growth speed.

Description

[0001] Technical field [0002] The present invention involves the field of plant planting, and it involves a fast indoor cultivation method of wild wheat. Background technique [0003] Wild Maima ( Hordeum brevisubularum (Trin.) Link) The perennial herbaceous plants belonging to the genus of Hercho. As one of the most salt -resistant salt -resistant plants in the Hercho wheat, they have a close relationship with barley and wheat.It has the characteristics of high yield, good parallelity, and high nutritional value, and has been used as an advantageous species of crop and pasture new varieties.At present, a hybridization experiment has been conducted with pastoral grass, which has cultivated No. 1 wild wheat with high production performance and strong salt resistance.However, because it belongs to the wild species, the biggest challenge when training indoors is the low seed germination rate, slow growth, and weak plants;It takes 40 to 50 days to grow to 4 leaves, and the leaves a...

Claims

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Application Information

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IPC IPC(8): A01G1/00A01C1/00A01G31/00
Inventor 王春梅田福平王晓力杨红善杨晓张茜胡宇伍国强袁慧君王旭荣褚敏路远
Owner LANZHOU INST OF ANIMAL SCI & VETERINARY PHARMA OF CAAS
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