Corn seed coating agent as well as preparation method and application thereof

Abstract

The invention relates to the technical field of crop cultivation, in particular to a corn seed coating agent and a preparation method and application thereof.The corn seed coating agent is prepared from, by mass, 0.01%-0.13% of red indazim canola, 0.01%-0.1% of nitrogen element, 0.06%-0.08% of phosphorus element, 0.15%-0.30% of potassium element, 0.04%-0.06% of organic carbon, 0.01%-0.03% of polypeptide, 2.8%-3.2% of difenoconazole and the balance water. The composition is prepared from the following raw materials in percentage by weight: 1.3-1.7% of carbofuran, 0.4-0.6% of a film-forming agent and the balance of water. After the corn seed coating agent provided by the invention is applied to corn seeds, the corn seeds are neat in seedling emergence, high in seedling vigor, vigorous in corn plant growth vigor, dense in corn root system and good in root hair development, the morbidity of corn stalk rot and corn ear rot is reduced, and the quality of the low-activity corn seeds is improved.

Description

Technical Field

[0001] The invention relates to the technical field of crop cultivation, and in particular to a corn seed coating agent and a preparation method and application thereof. Background Art

[0002] Corn is an important food crop, feed crop and industrial raw material crop in my country, and occupies a very important position in the national economy. Corn seeds are the basis of corn planting, and their quality, stress resistance and adaptability directly affect the growth and yield of corn. However, in actual applications, corn seeds have the problem of poor quality. These corn seeds with poor quality or quality degradation due to long-term storage face many challenges in the early growth stage, such as low germination rate, slow seedling growth, poor stress resistance, etc., which directly lead to a significant reduction in the yield and quality of subsequent corn crops.

[0003] Seed coating is the most important step in seed production. Seed coating agent is a product that is coated on the outer wall of seeds and has the purpose of preventing and controlling diseases. Seed coating agents mainly include chemical agents. Although they are helpful in preventing and controlling diseases and pests, they have a single effect. Long-term use will cause significant secondary pollution to the soil, water sources and ecological environment, affecting ecological balance and sustainable development.

[0004] At present, corn seed coating agents are mainly used to prevent and treat diseases, but cannot solve the problems of weak seedlings and poor root development caused by poor corn seed quality or quality degradation due to long-term storage. Therefore, it is urgent to prepare a coating agent for improving the quality of low-vigor corn seeds. Summary of the invention

[0005] In order to develop a method for improving the quality of low-vigor corn seeds, the present invention provides a corn seed coating agent and a preparation method and application thereof. After the corn seed coating agent provided by the present invention is used to improve low-vigor corn seeds (such as old corn seeds), the seeds emerge uniformly, the seedlings are high, the plants grow vigorously, the corn root system is dense, the root hairs are well developed, and the incidence of stem base rot and ear rot is low.

[0006] A corn seed coating agent is prepared from the following components, calculated by mass percentage of the corn seed coating agent: 0.10‰-1.3‰ of red indole and brassica, 0.01‰-0.1‰ of nitrogen, 0.06‰-0.08‰ of phosphorus, 0.15‰-0.30‰ of potassium, 0.04‰-0.06‰ of organic carbon, 0.1‰-0.3‰ of polypeptide, 2.8‰-3.2‰ of difenoconazole, 1.3‰-1.7‰ of carbofuran, 4‰-6‰ of film-forming agent, and the balance being water.

[0007] The amino acid sequence of the polypeptide is Tyr-Ile-Tyr-Glu-Val-Ala-Lys.

[0008] The corn seed coating agent provided by the present invention is processed by synergistic treatment of red indole, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide, and the obtained corn seeds show a more vigorous growth trend after coating. Specifically, the corn seeds have uniform seedlings, high seedling vigor, wide leaves, dense corn root system, and well-developed root hairs, which not only reduces the incidence of corn stem base rot and corn ear rot, but also improves the quality of low-vigor corn seeds.

[0009] Furthermore, calculated by mass percentage of the corn seed coating agent, the red indole brassica rapa is 1.2‰, nitrogen element is 0.075‰, phosphorus element is 0.075‰, potassium element is 0.18‰, organic carbon is 0.045‰, polypeptide is 0.2‰, fenpropimorph is 3.0‰, chlorpyrifos is 1.5‰, film-forming agent is 5‰, and the balance is water.

[0010] Furthermore, the nitrogen element is provided by a nitrogen-fixing alloy, the phosphorus element is provided by disodium hydrogen phosphate, the potassium element is provided by potassium chloride, and the organic carbon is provided by activated carbon.

[0011] The present invention provides a method for preparing a corn seed coating agent, comprising the following steps:

[0012] According to the mass-to-million dosage relationship, weigh red, indole, brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide, film-forming agent and water for later use; wherein the mass of the nitrogen alloy is calculated in terms of nitrogen element, the mass of potassium chloride is calculated in terms of potassium element, the mass of disodium hydrogen phosphate is calculated in terms of phosphorus element, and the mass of activated carbon is calculated in terms of carbon element;

[0013] Add red indole brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide and water in sequence and mix evenly to obtain a mixed solution, and then add a film-forming agent and mix evenly to obtain a corn coating agent.

[0014] Furthermore, the mixed solution is stirred at 100 r / min to 120 r / min at 15° C. to 25° C. during mixing.

[0015] Furthermore, after adding the film-forming agent, stirring is performed at 15° C. to 25° C. at 80 r / min to 100 r / min for 8 min to 12 min to mix evenly.

[0016] The invention also provides application of a corn seed coating agent in corn planting.

[0017] Furthermore, the corn seed coating agent is mixed and used in a ratio of 3 mL to 7 mL of coating agent: 0.8 kg to 1.2 kg of corn seeds.

[0018] Furthermore, the corn seed coating agent is used to promote the development of corn root system; increase leaf area, plant height and stem thickness.

[0019] Furthermore, the corn seed coating agent is used to prevent and control corn stalk rot and corn ear rot.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The corn seed coating agent provided by the invention is processed by synergistic treatment of erythrocarb, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide, so that the obtained corn seeds have uniform seedlings, high seedling vigor, vigorous plant growth, dense corn root system and well-developed root hairs, which not only reduces the incidence of corn stalk base rot and corn ear rot but also improves the quality of low-vigor corn seeds.

[0022] The corn seed coating agent provided by the present invention can not only improve the photosynthetic efficiency of corn plants, promote corn to synthesize more organic matter, and improve biomass accumulation through the coordinated treatment of red indole and brassica, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide; it can also enhance the transpiration of leaves, promote the transportation and balance of water and nutrients in the plant body. At the same time, the development of the corn root system expands the absorption area of ​​the corn root system in the soil, and improves the absorption efficiency of the corn root system for soil moisture and nutrients. In addition, the obvious improvement of plant height and stem thickness also enhances the anti-lodging ability and structural stability of the corn plant to a certain extent. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0024] Figure 1 This is a schematic diagram of the overall growth of corn seeds under different coating treatments; in the figure, A is 17-168-3; B is 17-168-1; C is 17-168-2; D is 17-168-CK; E is 17-H-CK; F is 17-H-2; G is 17-H-1; and H is 17-H-2.

[0025] Figure 2 Schematic diagram of the effect of different coating treatments on corn seed germination; in the figure, A is 17-168-3; B is 17-168-2; C is 17-168-1; D is 17-168-CK; E is 17-H-1; F is 17-H-2; G is 17-H-3; and H is 17-H-CK.

[0026] Figure 3 This is a schematic diagram of the effects of different coating treatments on the root system of corn seedlings. In the figure, A is 17-168-1; B is 17-168-2; C is 17-168-3; D is 17-168-CK; E is 17-H-1; F is 17-H-2; G is 17-H-3; and H is 17-H-CK.

[0027] Figure 4 This is a statistical chart showing the effects of different coating treatments on corn ear rot and stem base rot. DETAILED DESCRIPTION

[0028] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0029] Test Materials:

[0030] 17-168 and 17-H corn varieties, Zhangye Jinyuan Seed Co., Ltd.; red indole and brassica rapa, Agraforestry Agricultural and Forestry Environmental Biotechnology Co., Ltd., Germany; nitrogen alloy, Tianjin Comeo; potassium chloride, Tianjin Comeo; disodium hydrogen phosphate, Tianjin Comeo; activated carbon, analytical grade, Combis Bio-sugar-free tissue culture; fenpropimorph, Syngenta, Switzerland; carbofuran, Senchuang Pharmaceutical Biotechnology; polypeptide (amino acid sequence: YIYEVAK), Shanghai Shenggong Synthesis; film-forming agent (the main ingredient is acrylic acid), Syngenta Group Co., Ltd.

[0031] Example 1: A corn seed coating agent and a preparation method thereof.

[0032] Calculated in thousandths by mass of the corn seed coating agent, it includes 1.2‰ red indole, 0.075‰ nitrogen, 0.075‰ phosphorus, 0.18‰ potassium, 0.045‰ organic carbon, 0.2‰ polypeptide, 3.0‰ fenpropimorph, 1.5‰ chlorpyrifos, 5‰ film-forming agent and the balance is water.

[0033] Among them, the nitrogen element is provided by nitrogen-fixing alloy, the phosphorus element is provided by disodium hydrogen phosphate, the potassium element is provided by potassium chloride, and the organic carbon is provided by activated carbon.

[0034] The preparation steps of the corn seed coating agent are as follows:

[0035] (1) according to the mass ratio of each component in the above corn seed coating agent, red indole and brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide, film-forming agent and water are weighed and set aside; wherein the mass of nitrogen alloy is calculated as nitrogen element, the mass of potassium chloride is calculated as potassium element, the mass of disodium hydrogen phosphate is calculated as phosphorus element, and the mass of activated carbon is calculated as carbon element;

[0036] (2) Add red indole brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide and water in sequence according to the weight of the packed corn seeds and directly put them into the container and stir them evenly at 20°C and 110 rmin;

[0037] (3) Then add the film-forming agent and stir at 90 r / min for 10 min at 20°C. When the solution is evenly mixed, the corn seed coating agent is obtained.

[0038] Example 2: A corn seed coating agent and a preparation method thereof.

[0039] Calculated in thousandths by mass of the corn seed coating agent, it includes 1.3‰ red indole, 0.1‰ nitrogen, 0.08‰ phosphorus, 0.3‰ potassium, 0.06‰ organic carbon, 0.3‰ polypeptide, 3.2‰ fenpropimorph, 1.7‰ chlorpyrifos, 4‰ film-forming agent and the balance is water.

[0040] Among them, the nitrogen element is provided by nitrogen-fixing alloy, the phosphorus element is provided by disodium hydrogen phosphate, the potassium element is provided by potassium chloride, and the organic carbon is provided by activated carbon.

[0041] The preparation steps of the corn seed coating agent are as follows:

[0042] (1) according to the mass ratio of each component in the above corn seed coating agent, red indole and brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide, film-forming agent and water are weighed and set aside; wherein the mass of nitrogen alloy is calculated as nitrogen element, the mass of potassium chloride is calculated as potassium element, the mass of disodium hydrogen phosphate is calculated as phosphorus element, and the mass of activated carbon is calculated as carbon element;

[0043] (2) Add red indole brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide and water in sequence according to the weight of the seeds directly into the container and stir evenly at 110 rpm at 20°C;

[0044] (3) Then add the film-forming agent and stir at 90 r / min for 10 min at 20°C. When the solution is evenly mixed, the corn seed coating agent is obtained.

[0045] Example 3: A corn seed coating agent and a preparation method thereof.

[0046] Calculated by mass percentage of corn seed coating agent, it includes 0.1‰ red indole, 0.01‰ nitrogen, 0.06‰ phosphorus, 0.15‰ potassium, 0.04‰ organic carbon, 0.1‰ polypeptide, 2.8‰ fenpropimorph, 1.3‰ chlorpyrifos, 6‰ film-forming agent, and the remainder is water.

[0047] Among them, the nitrogen element is provided by nitrogen-fixing alloy, the phosphorus element is provided by disodium hydrogen phosphate, the potassium element is provided by potassium chloride, and the organic carbon is provided by activated carbon.

[0048] The preparation steps of the corn seed coating agent are as follows:

[0049] (1) according to the mass ratio of each component in the above corn seed coating agent, red indole and brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide, film-forming agent and water are weighed and set aside; wherein the mass of nitrogen alloy is calculated as nitrogen element, the mass of potassium chloride is calculated as potassium element, the mass of disodium hydrogen phosphate is calculated as phosphorus element, and the mass of activated carbon is calculated as carbon element;

[0050] (2) Add red indole brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide and water in sequence according to the weight of the packed corn seeds and directly put them into the container and stir them evenly at 20°C and 110 rmin;

[0051] (3) Then add the film-forming agent and stir at 90 r / min for 10 min at 20°C. When the solution is evenly mixed, the corn seed coating agent is obtained.

[0052] Comparative Example 1:

[0053] Calculated by mass percentage of the corn seed coating agent, the amount is 0.2‰ red indole, 0.2‰ polypeptide, 3.0‰ fenpropimorph, 1.5‰ chlorpyrifos, 5‰ film-forming agent, and the remainder is water.

[0054] The preparation steps of the corn seed coating agent are as follows:

[0055] (1) Weigh red indole, difenoconazole, carbofuran, polypeptide, film-forming agent and water according to the mass ratio of each component in the above corn seed coating agent, and set aside;

[0056] (2) weighing red indole, difenoconazole, carbofuran, polypeptide and water in accordance with the weight of the packed corn seeds in sequence, and directly placing them into a container and stirring them evenly at 20° C. and 110 r / min;

[0057] (3) Then add the film-forming agent and stir at 90 r / min for 10 min at 20°C. When the solution is evenly mixed, the corn seed coating agent is obtained.

[0058] Comparative Example 2:

[0059] Calculated by mass percentage of corn seed coating agent, the amount is 0.12‰ red indole, 3.0‰ fenpropimorph, 1.5‰ chlorpyrifos, 5‰ film-forming agent, and the remainder is water.

[0060] The preparation steps of corn seed coating agent are as follows:

[0061] (1) Weigh red indole, difenoconazole, carbofuran, film-forming agent and water according to the mass ratio of each component in the above corn seed coating agent, and set aside;

[0062] (2) weighing red indole brassica rapa, difenoconazole, carbofuran and water according to the weight of the seeds in turn and directly placing them into a container and stirring them evenly at 110 rpm at 20°C;

[0063] (3) Then add the film-forming agent and stir at 90 r / min for 10 min at 20°C. When the solution is evenly mixed, the corn coating agent is obtained.

[0064] The results produced by the corn seed coating agents prepared in Examples 1 to 3 are similar, and the specific results are subject to Example 1. The corn seed coating agents prepared in Example 1 and Comparative Examples 1 and 2 were used as research objects to study the effects of different corn seed coating agents on low-vigor corn seeds. The relevant test methods and results are as follows:

[0065] 1. Effects of different coating treatments on the emergence of low-vigor corn seeds

[0066] 1. Observation of seed emergence

[0067] 1.1 Test methods

[0068] Corn varieties: 17-168 and 17-H.

[0069] The corn coating agent of Example 1 was used to coat corn varieties 17-168 and corn variety 17-H, which were recorded as 17-168-1 and 17-H-1, respectively; the corn coating agents of Comparative Example 1 and Comparative Example 2 were used to coat corn variety 17-168, which were recorded as 17-168-2 and 17-168-3, respectively; the corn coating agents of Comparative Example 1 and Comparative Example 2 were used to coat corn variety 17-H, which were recorded as 17-H-2 and 17-H-3, respectively; at the same time, corn variety 17-168 and corn variety 17-H that were not coated were set as control groups, which were recorded as 17-168-CK and corn variety 17-H-CK, respectively.

[0070] The specific coating steps are: coat the prepared corn coating agent and corn seeds in a ratio of 5mL:1kg, then dry them, plant the corn seeds in a germination box, observe the germination after 5 days, and plant 90 plants in each box.

[0071] 1.2 Test results

[0072] There are obvious differences in the emergence of low-vigor corn seeds treated with different seed coating agents, such as Figure 1 and Figure 2 As shown, the emergence and growth of 17-H after three kinds of corn seed coating treatments were significantly different, among which 17-H-1 had the best emergence and growth, which was significantly different from 17-H-CK, 17-H-2 and 17-H-3. The emergence and growth of 17-H-2 and 17-H-3 were slightly different from 17-H-CK, and there was no significant change. The emergence and growth of 17-168 after three kinds of coating treatments were significantly different, among which 17-168-1 had high seedling vigor and wide corn leaves, and the seeds treated with 17-168-2 had poor emergence and dry leaf tips.

[0073] The results show that the corn seed coating agent provided by the present invention significantly promotes the germination and growth of corn seeds through the synergistic treatment of red indole, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide. Specifically, the seedling vigor of the corn seeds after germination is high, and the corn leaves are wide, which is significantly better than the other two groups of formulas; in addition, the wide corn leaves mean the improvement of the photosynthesis efficiency of the corn seeds and the improvement of biomass accumulation; at the same time, the corn seed coating agent provided by the present invention has slight differences in different varieties of corn seeds, but the overall trend is increasing, that is, all varieties of corn seeds show a more vigorous growth trend after coating.

[0074] 2. Effects of different coating treatments on root development of corn seedlings

[0075] 1. Observation of root development

[0076] 1.1 Test methods

[0077] The coated corn seeds of each group in the above experiment were planted in a germination box. When the corn grew to 4 leaves, the corn roots were carefully dug out with a shovel. After rinsing, the development of the corn roots was observed and scanned and measured with a root scanner.

[0078] 1.2 Test results

[0079] The root system of corn seedlings planted after treatment was scanned using a root system scanner. Figure 3The results showed that the corn 17-168 had well-developed lateral roots, dense root hairs and a well-developed root system after coating. The corn 17-168-CK had few lateral roots, sparse root hairs and slow root development. The corn seedlings of 17-168-1 had the best root development. The corn 17-H-2 and 17-H-3 had slow root development, fewer root hairs and shorter root systems. The corn 17-H-1 had faster root development, more root hairs and faster lateral root growth. The root system of 17-168-CK was sparse and had fewer root hairs.

[0080] As shown in Table 1, the root development of corn 17-168 after coating treatment was significantly different. Among them, the root development of corn 17-168-1 was the best after the seeds were coated with red, indole, brassica, nitrogen, phosphorus, potassium, organic carbon and peptides, and the root surface area was 59.74cm 2 , the root volume is 14.50cm 3 The root length was 316.18 cm and the number of root tips was 2551.33. The second was 17-168-2, which was treated with red, indole, brassica and peptides, and its root surface area was 46.38 cm 2 , the root volume is 11.34cm 3 , the root length was 207.89cm, and the number of root tips was 1171.00. The next one was 17-168-3, which was treated with red, indole, and brassica, and its root surface area was 31.39cm 2 , the root volume is 9.68cm 3 , the root length is 113.96 cm, and the number of root tips is 640.67. The root surface area of ​​17-168-CK is 18.34 cm 2 , the root volume is 5.78cm 3 , the root length is 84.51cm, and the number of root tips is 424.00.

[0081] The root development of 17-H corn was significantly different after the coating treatment. Among them, 17-H-1 corn had the best root development after the seeds were coated with red, indole, brassica, nitrogen, phosphorus, potassium, organic carbon and peptides. The root surface area was 47.91cm 2 , the root volume is 22.36cm 3 , with a root length of 263.40 cm and a root tip number of 1671.00. The second is 17-H-2, which has a root surface area of ​​37.50 cm after being treated with red, indole, brassica and peptides. 2 , the root volume is 13.81cm 3 , the root length was 193.00 cm, and the number of root tips was 1126.67. Next was 17-H-3, which was treated with red, indole, and brassica rapa, and its root surface area was 20.22 cm 2 , root system 9.36cm3 , the root length is 108.64 cm, and the number of root tips is 526.00. The root surface area of ​​17-H-CK is 11.98 cm 2 , the root volume is 7.00cm 3 , the root length is 63.60cm and the number of root tips is 244.

[0082] Table 1 Root development of different varieties of corn under each treatment

[0083] Group <![CDATA[Surface area / cm 2 > <![CDATA[Volume / cm 3 > Length / cm Number of root tips Example 1 17-168-1 59.74±0.75a 14.50±1.42a 316.18±1.12a 2551.33±60.70a Comparative Example 1 17-168-2 46.38±1.08b 11.34±1.53b 207.89±2.13b 1171.00±14.00b Comparative Example 2 17-168-3 31.39±0.40c 9.68±0.19b 113.96±4.03c 640.67±16.23c Comparative Example 3 17-168-CK 18.34±1.02d 5.78±0.20c 84.51±13.41d 424.00±7.00d Example 4 17-H-1 47.91±0.76a 22.36±2.30a 263.40±5.59a 1671.00±67.29a Comparative Example 4 17-H-2 37.50±2.09b 13.81±1.88b 193.00±8.69b 1126.67±122.79b Comparative Example 5 17-H-3 20.22±2.72c 9.36±0.53c 108.64±2.37c 526.00±12.17c Comparative Example 6 17-H-CK 11.98±1.41c 7.00±0.99c 63.60±4.76d 244.00±14.73d

[0084] Note: Different lowercase letters in the same row indicate significant differences among treatments at the p<0.05 level.

[0085] In summary, the corn seed coating agent provided by the present invention significantly promotes the growth of the root system of corn seeds through the synergistic treatment of red indole and brassica, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide. Specifically, after treatment, the lateral roots of the corn root system are well-developed, the root hairs are dense, and the root system is well-developed, which can expand the absorption area of ​​the corn root system in the soil and improve the absorption efficiency of the corn root system for soil moisture and nutrients. At the same time, the corn seed coating agent provided by the present invention has slight differences in the varieties of different corn seeds, but the overall trend is increasing, that is, all varieties of corn seeds show a more vigorous growth trend after coating.

[0086] 3. Effects of different treatments on agronomic traits of corn

[0087] 1. Observation of agronomic traits

[0088] 1.1 Test methods

[0089] The corn seeds of each group after the above treatment were planted in the Gansu beer barley original seed farm. The planting plot was 4.0m long and 1.4m wide, with 3 replicates for each variety. The field management was normal, and the plant height, stem diameter and SPAD value were measured during the corn silking period. Plant height: the height of the main stem of the plant from the ground to the top of the tassel, in cm; stem diameter: the diameter of the middle stem (excluding leaf sheath) of each internode (3-7) of the plant stem was measured with an electronic vernier caliper, and the average value was taken as the thickness, stem diameter = (longest diameter + shortest diameter) / 2, in mm; SPAD: the chlorophyll content of the second leaf leaf was measured using a portable chlorophyll meter, and 3 points were taken on each leaf.

[0090] 1.2 Test results

[0091] As shown in Table 2, after the three coating treatments, the agronomic traits of different varieties of corn in the field were significantly different. Among them, the agronomic traits of 17-168 were significantly different after coating treatment. The leaf area of ​​corn after 17-168-1 coating treatment was: 644.44cm 2, plant height: 167.34cm, stem diameter: 30.52cm, SPAD value: 47.87; corn leaf area after 17-168-2 coating treatment: 523.00cm 2 , plant height: 154.67cm, stem diameter: 25.86cm, SPAD value: 44.60; corn leaf area after 17-168-3 coating treatment: 482.90cm 2 , plant height: 130.67cm, stem diameter: 21.58cm, SPAD value: 41.67; 17-168 corn agronomic traits significantly decreased after no coating treatment, leaf area: 314.38cm 2 , plant height: 112.67cm, stem diameter: 21.91cm, SPAD value: 37.83.

[0092] The agronomic traits of 17-H after coating treatment were significantly different. The leaf area of ​​corn after 17-H-1 coating treatment was: 555cm 2 , plant height: 167.67cm, stem diameter: 35.32cm, SPAD value: 53.50; corn leaf area after 17-H-2 coating treatment: 474.98cm 2 , plant height is 147.67cm, stem diameter is 31.95cm, SPAD value is 47.90; corn leaf area after 17-H-3 coating treatment is: 407.23cm 2 , plant height: 132.33cm, stem diameter: 24.33cm, SPAD value: 42.63; 17-H corn agronomic traits were reduced after uncoated treatment, leaf area: 332.80cm 2 , plant height is 123.67cm, stem diameter is 21.98cm, and SPAD value is 40.63.

[0093] Table 2 Effects of each treatment on agronomic traits of different corn varieties

[0094] Group <![CDATA[Leaf area / cm 2 > Plant height / cm Stem diameter / cm SPAD Example 1 17-168-1 644.44±1.63a 167.34±1.53a 30.52±1.32a 47.87±1.59a Comparative Example 1 17-168-2 523.00±6.31b 154.67±4.04b 25.86±1.02b 44.60±0.17b Comparative Example 2 17-168-3 482.90±10.14c 130.67±4.51c 21.58±0.36c 41.67±0.57c Comparative Example 3 17-168-CK 314.38±9.78d 112.67±0.58d 21.91±1.32c 37.83±0.38d Example 4 17-H-1 555.00±12.99a 167.67±2.08a 35.32±1.34a 53.50±0.87a Comparative Example 4 17-H-2 474.98±2.52b 147.67±2.08b 31.95±1.31b 47.90±0.80b Comparative Example 5 17-H-3 407.23±5.98c 132.33±2.52c 24.33±0.85c 42.63±1.17c Comparative Example 6 17-H-CK 332.80±2.25d 123.67±0.58d 21.98±1.52c 40.63±0.76d

[0095] Note: Different lowercase letters in the same row indicate significant differences among treatments at the p<0.05 level.

[0096] The results show that the corn seed coating agent provided by the present invention significantly improves the leaf area, plant height and stem thickness of corn after germination through the coordinated treatment of red indole ethyl brassica, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide. The increase in corn leaf area can not only enhance the photosynthesis efficiency of leaves and promote the synthesis of more organic matter by corn leaves, but also enhance the transpiration of leaves, and promote the transportation and balance of water and nutrients in the plant. In addition, the plant height and stem thickness are significantly improved, and the lodging resistance and structural stability of corn plants are also enhanced to a certain extent.

[0097] 17-168-1, 17-168-2 and 17-168-3 were selected to study the effects of different coating treatments on corn ear rot and stem base rot. The specific test steps and test results are as follows:

[0098] 1. Observation of disease occurrence in corn fields

[0099] 1.1 Test methods

[0100] The survey was conducted when corn entered the late milky stage, and the disease status of the 17-168-H variety was investigated plant by plant. Stem base rot: The stem nodes or internodes turned brown or dark brown, the stem nodes became soft, and the pith was hollow and darker when the pith was cut open, indicating that the plant was infected. Ear rot: Tear open the bracts and observe whether the corn ear is damaged by cotton bollworms and whether there is hyphae growth on the ear. Record the total number of plants investigated and the number of infected plants, and calculate the disease rate.

[0101] 1.2 Test results

[0102] like Figure 4 As shown, the incidence of ear rot and stem base rot in corn fields after different coating treatments was significantly different. The incidence of ear rot and stem base rot in 17-168 after coating treatment was significantly lower than that in 17-168-CK. Among them, the incidence of ear rot and stem base rot in corn after coating treatment with 17-168-1 was the lowest, at 0.2% and 0.5%, respectively.

[0103] The results show that the corn seed coating agent provided by the present invention can effectively prevent and control corn field ear rot and stem base rot through the synergistic treatment of red indole, nitrogen, potassium, phosphorus, organic carbon, difenoconazole, carbofuran and polypeptide.

[0104] Although preferred embodiments of the present invention have been described, additional changes and modifications may occur to these embodiments once those skilled in the art are aware of the basic inventive concepts.

[0105] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A corn seed coating agent, characterized in that: The corn seed coating agent is made of the following components, calculated by mass percentage: 0.10‰-1.3‰ red indole and brassica, 0.01‰-0.1‰ nitrogen, 0.06‰-0.08‰ phosphorus, 0.15‰-0.30‰ potassium, 0.04‰-0.06‰ organic carbon, 0.1‰-0.3‰ polypeptide, 2.8‰-3.2‰ difenoconazole, 1.3‰-1.7‰ carbofuran, 4‰-6‰ film-forming agent, and the balance is water; The amino acid sequence of the polypeptide is Tyr-Ile-Tyr-Glu-Val-Ala-Lys.

2. The corn seed coating agent according to claim 1, characterized in that Calculated by mass percentage of the corn seed coating agent, the red indole and brassica rapa is 1.2‰, nitrogen element is 0.075‰, phosphorus element is 0.075‰, potassium element is 0.18‰, organic carbon is 0.045‰, polypeptide is 0.2‰, difenoconazole is 3.0‰, chlorpyrifos is 1.5‰, film-forming agent is 5‰, and the balance is water.

3. The corn seed coating agent according to claim 1, characterized in that The nitrogen element is provided by a nitrogen-fixing alloy, the phosphorus element is provided by disodium hydrogen phosphate, the potassium element is provided by potassium chloride, and the organic carbon is provided by activated carbon.

4. A method for preparing the corn seed coating agent according to claim 3, characterized in that: The steps include: According to the mass-to-million dosage relationship, weigh red, indole, brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide, film-forming agent and water for later use; wherein the mass of the nitrogen alloy is calculated in terms of nitrogen element, the mass of potassium chloride is calculated in terms of potassium element, the mass of disodium hydrogen phosphate is calculated in terms of phosphorus element, and the mass of activated carbon is calculated in terms of carbon element; Add red indole brassica, nitrogen alloy, potassium chloride, disodium hydrogen phosphate, activated carbon, difenoconazole, carbofuran, polypeptide and water in sequence, mix well to obtain a mixed solution, and then add a film-forming agent and mix well to obtain a corn coating agent.

5. The method for preparing the corn seed coating agent according to claim 4, characterized in that: The mixed solution is stirred at 100 r / min to 120 r / min at 15° C. to 25° C. during mixing.

6. The method for preparing the corn seed coating agent according to claim 4, characterized in that: After adding the film-forming agent, stir at 80 r / min to 100 r / min for 8 min to 12 min at 15° C. to 25° C. to mix evenly.

7. Use of the corn seed coating agent according to any one of claims 1 to 3 in corn planting.

8. Use of the corn seed coating agent according to claim 7 in corn planting, characterized in that: The corn seed coating agent is mixed and used in a ratio of 3 mL to 7 mL of coating agent: 0.8 kg to 1.2 kg of corn seeds.

9. The use of the corn seed coating agent in corn planting according to claim 7, characterized in that: The corn seed coating agent is used for promoting the development of corn root system and increasing leaf area, plant height and stem thickness.

10. The use of the corn seed coating agent in corn planting according to claim 7, characterized in that: The corn seed coating agent is used for preventing and controlling corn stalk base rot and corn ear rot.

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