Corn gluten meal functional fermented feed and application thereof

A corn gluten meal, fermented feed technology, applied in applications, animal feed, animal feed, etc., can solve the problems of low protein digestion and utilization rate, unbalanced amino acid composition, poor palatability, etc., and achieve great social value and intestinal tract. The effect of bacterial flora balance and improvement of disease resistance

Pending Publication Date: 2017-08-04
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Corn gluten powder contains about 60% protein, mainly gliadin, poor solubility, unbalanced amino acid c

Method used

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  • Corn gluten meal functional fermented feed and application thereof
  • Corn gluten meal functional fermented feed and application thereof
  • Corn gluten meal functional fermented feed and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0036] Example 1

[0037] Activation of strains and preparation and cultivation of seed liquid

[0038] 1) Activation of strains

[0039] Preparation of activation medium

[0040] The Bacillus natto activation medium is an LB solid medium, specifically tryptone 1%, sodium chloride 1%, yeast powder 0.5%, agar 2%, and the pH is adjusted to 7.0-7.4.

[0041] The saccharomyces cerevisiae activation medium is a wort solid medium. Specifically, 1000 mL of wort is used to adjust the sugar content to 8-10 Bx with an Abbe refractometer, the agar is 2%, and the pH is in a natural state.

[0042] The activation medium of Lactobacillus thermophilus is MRS lactic acid bacteria medium, specifically peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, Tween 801.0mL, acetic acid Sodium 5.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, and agar 18.0g are dissolved in 1000mL of distilled water, and the pH is adj...

Example Embodiment

[0048] Example 2

[0049] Solid fermentation of three strains of Bacillus subtilis, Bacillus coagulans and Bacillus natto

[0050] Medium preparation requirements: corn gluten meal: bran=7:3; material (that is, total weight of corn gluten meal and bran): water is mixed in a ratio of 1g:1.2ml to obtain a fermentation medium.

[0051] The inoculation amount of the strain is 10%, and the ratio of the number of viable cells of Bacillus subtilis, Bacillus coagulans and Bacillus natto is 1:1:1 to be inoculated into the fermentation medium for fermentation culture, and the temperature of fermentation culture is At 30°C, the fermentation culture time is 120h to obtain fermented feed.

Example Embodiment

[0052] Example 3

[0053] Bacillus subtilis, thermophilic lactic acid bacteria and Bacillus natto three kinds of combined solid fermentation

[0054] Medium preparation requirements: corn gluten meal: bran is 6:4; materials (that is, the total weight of corn gluten meal and bran) and water are mixed in a ratio of 1g:1.4mL to obtain a fermentation medium.

[0055] The inoculation amount of the strain is 8%, and the ratio of the viable cells of Bacillus subtilis, Lactobacillus thermophilus and Bacillus natto is 1:0.5:1.5 to be inoculated into the fermentation medium for fermentation culture, and the temperature of fermentation culture is At 32°C, the fermentation culture time is 108h to obtain fermented feed.

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Abstract

The invention provides corn gluten meal functional fermented feed and a preparation method and application thereof and belongs to the technical field of feed. The corn gluten meal functional fermented feed is a fermentation product of a culture medium containing corn gluten meal and bran under fermentation action of three bacteria. The three bacteria include bacillus subtilis and two of bacillus coagulans, thermophilic lactobacillus, bacillus natto and bear yeast. Soluble protein content in the fermented feed is increased from 3.74% to 270% at most; the novel microbial fermentation feed prepared by adding highly-symbiotic bacteria can bring about more economic benefit and has great social value. By applying the corn gluten meal functional fermented feed in animal raising, growing performance of raised animals is improved effectively while own immunity of the animals can be improved.

Description

technical field [0001] The invention belongs to the technical field of feed, in particular to corn gluten powder functional fermented feed and application thereof. Background technique [0002] With the development of my country's animal husbandry, the contradiction of protein feed shortage has become increasingly prominent. The main way to solve this contradiction is to develop new feed resources and rationally utilize existing feed resources. The production of high-protein feed by microbial fermentation is one of the effective ways to solve the shortage of feed protein resources. [0003] Corn is one of the three major food crops in the world today. In 2015, my country's corn planting area was 38,116.6 thousand hectares, with a unit yield of 5891.9Kg / ha, slightly higher than the world average. The main component of corn is starch, up to 75%, so corn plays an important role in starch production. At present, the main method of extracting corn starch in China is wet extrac...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/37A23K20/147A23K50/30
CPCY02P60/87
Inventor 刘晓兰江成英王燕郑喜群任健
Owner QIQIHAR UNIVERSITY
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