Corn gluten meal functional fermented feed and application thereof
A corn gluten meal, fermented feed technology, applied in applications, animal feed, animal feed, etc., can solve the problems of low protein digestion and utilization rate, unbalanced amino acid composition, poor palatability, etc., and achieve great social value and intestinal tract. The effect of bacterial flora balance and improvement of disease resistance
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[0036] Example 1
[0037] Activation of strains and preparation and cultivation of seed liquid
[0038] 1) Activation of strains
[0039] Preparation of activation medium
[0040] The Bacillus natto activation medium is an LB solid medium, specifically tryptone 1%, sodium chloride 1%, yeast powder 0.5%, agar 2%, and the pH is adjusted to 7.0-7.4.
[0041] The saccharomyces cerevisiae activation medium is a wort solid medium. Specifically, 1000 mL of wort is used to adjust the sugar content to 8-10 Bx with an Abbe refractometer, the agar is 2%, and the pH is in a natural state.
[0042] The activation medium of Lactobacillus thermophilus is MRS lactic acid bacteria medium, specifically peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, diammonium hydrogen citrate 2.0g, glucose 20.0g, Tween 801.0mL, acetic acid Sodium 5.0g, dipotassium hydrogen phosphate 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.25g, and agar 18.0g are dissolved in 1000mL of distilled water, and the pH is adj...
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[0048] Example 2
[0049] Solid fermentation of three strains of Bacillus subtilis, Bacillus coagulans and Bacillus natto
[0050] Medium preparation requirements: corn gluten meal: bran=7:3; material (that is, total weight of corn gluten meal and bran): water is mixed in a ratio of 1g:1.2ml to obtain a fermentation medium.
[0051] The inoculation amount of the strain is 10%, and the ratio of the number of viable cells of Bacillus subtilis, Bacillus coagulans and Bacillus natto is 1:1:1 to be inoculated into the fermentation medium for fermentation culture, and the temperature of fermentation culture is At 30°C, the fermentation culture time is 120h to obtain fermented feed.
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[0052] Example 3
[0053] Bacillus subtilis, thermophilic lactic acid bacteria and Bacillus natto three kinds of combined solid fermentation
[0054] Medium preparation requirements: corn gluten meal: bran is 6:4; materials (that is, the total weight of corn gluten meal and bran) and water are mixed in a ratio of 1g:1.4mL to obtain a fermentation medium.
[0055] The inoculation amount of the strain is 8%, and the ratio of the viable cells of Bacillus subtilis, Lactobacillus thermophilus and Bacillus natto is 1:0.5:1.5 to be inoculated into the fermentation medium for fermentation culture, and the temperature of fermentation culture is At 32°C, the fermentation culture time is 108h to obtain fermented feed.
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