Fish scale collagen and preparation method and application thereof, and ice cream rich in fish scale collagen and preparation method of ice cream
A technology of fish scale collagen and ice cream, applied in the field of fish scale collagen and its preparation, ice cream rich in fish scale collagen and its preparation, can solve the problems of low proteolysis degree and soluble protein content, and achieve improved stability and improved The effect of the degree of proteolysis and high nutritional value
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[0024] In a first aspect, the present invention provides a method for preparing fish scale collagen, the method comprising:
[0025] (1) fish scales are placed in acid solution for decalcification treatment to obtain decalcified fish scales;
[0026] (2) adding water to the decalcified fish scales, and extracting the fish gelatin under heating conditions to obtain a fish gelatin extract;
[0027] (3) performing solid-liquid separation on the fish gelatin extract to obtain fish gelatin;
[0028] (4) Contacting the fish gelatin with alkaline protease and trypsin in sequence to perform hydrolysis with alkaline protease and trypsin respectively, to obtain the fish scale collagen.
[0029] According to the present invention, in step (1), the acid may be any acid capable of decalcifying the fish scales, preferably, the acid is hydrochloric acid and / or citric acid.
[0030] Wherein, the amount of the acid can be changed within a relatively large range, as long as the fish scales ca...
Embodiment approach
[0048] According to a preferred embodiment of the present invention, the hydrolysis of the fish gelatin by alkaline protease is carried out in an aqueous solution, wherein the amount of water used is preferably such that the concentration of the alkaline protease is 0.1-0.5% by weight.
[0049] According to the present invention, the hydrolysis conditions of the alkaline protease can be conventional alkaline protease action conditions, preferably, the temperature is 45-55° C., the time is 3-4 hours, and the pH value is 7.5-8.5.
[0050] According to the present invention, preferably, before the product hydrolyzed by alkaline protease is subjected to trypsin hydrolysis, the method further includes inactivating alkaline protease, for example, by heating.
[0051] Further preferably, the method further includes solid-liquid separation (for example, centrifugation) of the alkaline protease hydrolyzed product, and drying the obtained liquid phase into powder. Wherein, the drying is...
Embodiment 1-1
[0084] This embodiment is used to illustrate the preparation of fish scale collagen
[0085] (1) Fish scale treatment: buy the remaining fish scales from the aquatic products company, beat them intermittently for 2 minutes with a tissue grinder after removing impurities, and rinse them with clean water several times. According to the ratio of fish scale: hydrochloric acid (concentration: 0.3mol / L) 1:5, put the fish scale in the hydrochloric acid solution at room temperature for 2.5 hours, and stir for 1 minute every 15 minutes during the decalcification period. After the decalcification of fish scales is completed, rinse and soak them repeatedly with clean water until the pH is washed to neutral.
[0086] (2) Gelatin extraction: Add distilled water according to the volume ratio of fish scale: water 1:5, heat in a water bath at 75°C for 2 hours, and stir continuously with a mechanical mixer during this period.
[0087] (3) Concentration and drying: the residue of fish scales w...
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