Method adopting rice residues as raw material for enzyme-membrane combined preparation of rice polypeptides

Abstract

The invention discloses a method adopting rice residues as the raw material for enzyme-membrane combined preparation of rice polypeptides. The method comprises steps as follows: installation of two enzyme reactors; weak-acid pretreatment of rice residue protein; primary enzymolysis adopting alkaline protease; secondary enzymolysis adopting flavourzyme; decolorization and desalination; concentration; spray drying. The method has the benefits as follows: mixed weak acid mild heat treatment is firstly adopted to improve solubility of rice protein so as to accelerate follow-up enzymatic hydrolysis, then by means of two times of enzymolysis adopting incision enzyme and excision enzyme sequentially, the degree of proteolysis can be increased, the bitter taste of products is reduced, the yield of the products is increased, and the production cost is remarkably reduced; an enzymolysis system directly passes through an ultrafiltration membrane, so that protease can be rapidly recycled in real time, the use amount of enzyme is reduced, the continuous operation of enzymolysis is realized, a traditional step of enzyme deactivation through heating can be omitted, accordingly, energy is greatly saved, the equipment configuration is simplified, and the overall production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of grain deep processing, and relates to a method for preparing rice polypeptide by using rice dregs as a raw material and enzymatic membrane. Background technique [0002] Rice is the largest grain variety in my country. The annual planting area accounts for about 30% of the total grain planting area, and the annual output is about 40 million tons. In addition to being directly eaten by people, rice is also used as an important raw material for industrial fermentation, starch sugar production and other industries. Enterprises that produce organic acids, antibiotics, and starch sugars using rice and broken rice as raw materials will produce about 10 million tons of by-products such as rice dregs and rice bran every year, which cause a great waste of resources due to failure to develop and utilize them well . Taking rice dregs as an example, its protein content is above 60%. The amino acid ratio of rice pr...

AI Technical Summary

Problems solved by technology

Therefore, the solubility of rice protein is poor, and the difficulty of enzymatic hydrolysis is further increased. The degree of hydrolysis of the product obtained by enzymatic hydrolysis with an enzyme is low, generally below 25%.

On the other hand, there is no recycling process for enzymes in the enzymatic hydrolysis process, resulting in low enzyme utilization rate, which cannot be continuously produced, and deactivating enzymes consumes a lot of energy

There are a few reports that the method of using immobilized enzymes can improve the utilization rate of enzymes, but there are disadvantages such as high cost and uneven enzyme action.

Finally, the existing technology generally lacks the debittering treatment of polypeptides, which leads to the bitterness of the product and affects the quality of the product.