Strain of endophytic phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing acinetobacter calcoaceticus and application thereof

A technology of calcium acetate and bacilli, applied in the field of microorganisms, to achieve the effects of good strain resources, increasing photosynthetic rate, and increasing chlorophyll content

Active Publication Date: 2018-09-07
福州睿谷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are no reports on the isolation of culturable endophytic bacteria from different tissues of moso bamboo, the screening of endophytic bacteria with phosphorus, potassium, and nitrogen fixation functions, and the functional endophytic bacteria that can promote photosynthesis.

Method used

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  • Strain of endophytic phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing acinetobacter calcoaceticus and application thereof
  • Strain of endophytic phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing acinetobacter calcoaceticus and application thereof
  • Strain of endophytic phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing acinetobacter calcoaceticus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening of endogenous Phosphorus-dissolving, Potassium-dissolving, Nitrogen-fixing Calcium-Acetate Acinetobacter for Promoting Photosynthesis of Phyllostachys pubescens

[0026] (1) Screening process:

[0027] By gradient dilution method, 82 isolates were isolated from Moso bamboo central production areas of Wuyishan City, Changting County and Jiangle County, Fujian Province. The isolated endophytic bacteria were purified by the three-section line method, and whether the strain was purified was judged by microscopic examination. The purified bacteria were numbered, and single colonies were picked and transferred to the NA slope for future use. 20 strains of endophytic bacteria with good phosphorus, potassium, and nitrogen fixation effects were initially screened out through the determination of phosphorus, potassium, and nitrogen fixation effects on a plate, and one strain of endophytic bacteria with good phosphorus, potassium, and nitrogen fixation effects ...

Embodiment 2

[0056] Embodiment 2 Pot experiment

[0057] will promote the photosynthesis of moso bamboo Acinetobacter calcoaceticus ) WYS-A01-1 was activated on the NA slope, and a ring was picked and inserted into the NA medium (3g of beef extract, 10g of peptone, 5g of NaCl, 18g of agar, 1000ml of water, pH7.0-7.2), at 28℃, 180 rpm -1 Under the condition of shaking culture for 72h, the culture solution was diluted with sterile water to make 1×10 8 cfu / mL bacterial suspension for inoculation. The net photosynthetic rate, transpiration rate, stomatal conductance and intercellular CO 2 Determination of photosynthesis indicators such as concentration.

[0058] The photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs) and intercellular CO of bamboo leaves were measured 15 days and 30 days after the potted seedlings of Moso bamboo were inoculated with endophytic bacteria WYS-A01-1. 2 Concentration (Gs). The results showed that the photosynthetic rate (Pn), tra...

Embodiment 3

[0072] Embodiment 3 field experiment

[0073] Select the moso bamboo forest base in Longqishan Nature Reserve, Jiangle County, Fujian Province, select the moso bamboo of degree II, first use an electric drill to drill holes in the bamboo stems about 30cm away from the soil surface, and then take 30 mL of the above bacterial suspension and inject them with sterile syringes To the inside of the bamboo cavity of Moso bamboo, repeat inoculation with 30 mL the next day, and seal the cavity of the bamboo cavity with soil. 10 plants per treatment were repeated 3 times, and water was used as the control. 15d and 30d after treatment, 5-10 leaves of Moso bamboo in the four directions of east, south, west and north were collected by high branch shears respectively, and fully mixed as a mixed sample for chlorophyll content, catalase (CAT ), malondialdehyde (MDA), peroxidase (POD), superoxide dismutase (SOD), soluble protein content, soluble sugar and other physiological and biochemical i...

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Abstract

The invention provides a strain of endophytic phosphate-solubilizing, potassium-solubilizing and nitrogen-fixing acinetobacter calcoaceticus and application thereof. The acinetobacter calcoaceticus WYS-A01-1 was deposited in China General Microbiological Culture Collection Center (CGMCC) on July 3, 2017, and the preservation number is: CGMCC No.14378. The endophytic acinetobacter calcoaceticus ofphyllostachys edulis has the functions of solubilizing phosphate, solubilizing potassium and fixing nitrogen, can colonize plants, promote photosynthesis of the phyllostachys edulis, improve biological enzyme activity, and play an important role in regulating plant growth and development.

Description

technical field [0001] The invention belongs to the field of microorganisms, and relates to an endogenous phosphorus-dissolving, potassium-dissolving, nitrogen-fixing, calcium-acinetobacter Acinetobacter for promoting the photosynthesis of moso bamboo and its application. Background technique [0002] bamboo( Phyllostachys edulis ) is an important forest resource in southern China. It is a scattered bamboo species and has the advantages of fast growth, early maturity, wide application, and large economic benefits. According to the statistics of the 6th National Forest Resources Inventory by the State Forestry Administration, the bamboo forest area in 10 provinces (autonomous regions and municipalities directly under the Central Government) including Fujian, Jiangxi, and Zhejiang accounted for the total area of ​​bamboo forest in the country (4.8426 million hm 2 ) of 93.78%. Among them, the moso bamboo is about 3.372 million hm 2 , accounting for 70% of the country's bam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C05F11/08C12R1/01
CPCA01N63/00C05F11/08C12N1/20C12N1/205C12R2001/01
Inventor 袁宗胜刘芳张国防谢宝贵
Owner 福州睿谷生物科技有限公司
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