The invention discloses an OLED (Organic Light-Emitting Diode)- and OPD (Organic Photodetector)-based cascading vertical integration capillary electrophoresis chip. The OLED- and OPD-based cascading vertical integration capillary electrophoresis chip comprises arrayed organic thin-film light sources (OLEDs), a polarizer, a multi-channel capillary electrophoresis chip, a polarization detector and arrayed organic thin-film detectors (OPDs), wherein the OLEDs, the polarizer, the multi-channel capillary electrophoresis chip, the polarization detector and the OPDs are sequentially placed in a parallel and cascading manner; and an upper part and a lower part of each electrophoresis channel detection position respectively correspond to the single OLED and the single OPD. According to the OLED- and OPD-based cascading vertical integration capillary electrophoresis chip, the OLEDs and the OPDs which are made from organic photoelectric materials respectively are adopted, therefore, the easy integration with the multi-channel capillary electrophoresis chip is achieved; with the adoption of planar structural parallel cascading arrangement, the distance between every two devices is small, the optical length of a light path in transmission along the vertical direction is short, the optical loss is effectively lowered, the improvement of system sensitivity is facilitated, and the accuracy in detection is improved through the controlling of the diameter of an exciting light beam by a pinhole; and polaroids which are arranged in a mutually-vertical manner are used for light-filtering, excitation light and stray light are effectively filtered out, therefore, the OLED- and OPD-based cascading vertical integration capillary electrophoresis chip is applicable to a detection system with wider spectral range.