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Apparatus and cultivating method for scaled cultivation of microalgae

A large-scale, microalgae technology, applied in the direction of microorganism-based methods, stress-stimulated microbial growth methods, enzymatic/microbiological devices, etc., can solve the limitations of large-scale applications, limited number of reactors, reactor investment and operating costs Problems such as high cost, to achieve the effect of increasing cell production speed, solving efficiency/cost, and reducing temperature control cost

Active Publication Date: 2013-06-19
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, through open pond cultivation, although the investment and operating costs are low, the rapid growth and high photosynthetic carbon fixation capabilities of microalgae are far from being fully utilized. However, photoreactor cultivation can achieve rapid and high-density microalgae in the reactor. growth and high photosynthetic carbon fixation, but due to the structural characteristics of the photoreactor itself and the requirements for light, the reactors must be arranged at intervals, and the number of reactors per unit land area is limited, so the average area of ​​microalgae yield is also low. Very high, and reactor investment and operating costs are high
It is for these reasons based on efficiency / cost that microalgae cultivation has many disadvantages whether it is open or closed, which limits its large-scale application

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  • Apparatus and cultivating method for scaled cultivation of microalgae
  • Apparatus and cultivating method for scaled cultivation of microalgae

Examples

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Effect test

Embodiment 1

[0039] Two elliptical track pools with a length of 3.8 meters and a width of 1.9 meters are used for outdoor cultivation of Nanochoropsis. Its filling height is 0.25 meters (1500 liters of filling volume), and the stirring water velocity is 0.25 meters per second. Nitrogen-enriched f / 2 medium (NaNO 3 Concentration 0.75 g / L), cell seeding density 0.1 g / L. One of the runway pools is connected with a flat-plate photoreactor prepared by a plexiglass plate with a liquid capacity of 0.05 meters, a height of 1.2 meters, and a width of 2.4 meters by a water pump. The culture solution on one side of the open pool is pumped from the bottom of one side of the flat photoreactor, and then flows back to the other side of the open pool from the upper part of the other side of the flat photoreactor, and the flow rate of the water pump is controlled to 1000 liters / hours to keep the average residence time of the culture medium in the flat photobioreactor at 45 seconds. Flat-plate photoreact...

Embodiment 2

[0041]Two elliptical track pools with a length of 3.8 meters and a width of 1.9 meters are used for outdoor Nanchoropsis cultivation. Its filling height is 0.25 meters (1500 liters of filling volume), and the stirring water velocity is 0.25 meters per second. Nitrogen-enriched f / 2 medium (NaNO 3 Concentration 0.75 g / L), cell seeding density 0.1 g / L. One of the runway pools is connected with two flat photoreactors with a thickness of 0.05 meters, a height of 1.2 meters, a width of 2.4 meters, and a plexiglass plate with a liquid capacity of 125 liters. The culture solution on one side of the open pool is pumped from the bottom of one side of the flat photoreactor, and then flows back to the other side of the open pool from the upper part of the other side of the flat photoreactor, and the flow rate of the water pump is controlled to 1500 liters / hours to keep the average residence time of the culture medium in the flat photobioreactor at 30 seconds. Flat-plate photoreactor i...

Embodiment 3

[0043] Two elliptical track pools with a length of 3.8 meters and a width of 1.9 meters are used for outdoor chlorella cultivation. Its filling height is 0.25 meters (1500 liters of filling volume), and the stirring water velocity is 0.25 meters per second. Nitrogen-enriched f / 2 medium (NaNO 3 Concentration 0.75 g / L), cell seeding density 0.15 g / L. One of the runway pools passes through a water pump with a flow rate of 1000 liters / hour, and then branches into 6 bubble column photoreactors with a diameter of 160 mm and a height of 1.8 meters (the liquid volume of each bubble column reactor is 30 liters) , the culture solution returns to the runway pool, and the average residence time of the culture solution in each bubble column photoreactor is 11 seconds. Bubble column photoreactor fed with air / CO 2 Mixed gas (5% CO 2 , v / v), the ventilation volume is 0.3VVM. During the cultivation period, the average temperature and light intensity are: 15°C at 8:00, 950umol / m 2 / s, 35°...

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Abstract

The invention relates to an apparatus and a cultivating method for scaled cultivation of microalgae. The apparatus comprises: an open-type track tank; a liquid outlet arranged on the open-type track tank; a liquid pump connecting to the liquid outlet, wherein a liquid inlet on the bottom of a photobioreactor is connected to the liquid outlet through the liquid pump; a liquid outlet tube on the top of the photobioreactor, wherein culture solution is delivered back to the open-type track tank through the tube. The cultivating method comprises the steps that: part of the culture solution in the open-type track tank is pumped into the bottom of the photobioreactor through the liquid pump; the culture solution is delivered from the top of the photobioreactor back to the open-type track tank, such that a circulation of culture solution is realized; the bottom of the photobioreactor is ventilated, and carbon is filled. With the apparatus and the method provided by the present invention, photosynthetic carbon fixation efficiency of open-type track tank microalgae cultivation is substantially improved, microalgae growth rate and cultivating density are substantially improved, cultivating period is reduced, and apparatus cost and operation cost of scaled microalgae cultivation are reduced.

Description

technical field [0001] The invention belongs to the field of microalgae cultivation, in particular to a device for large-scale cultivation of microalgae. [0002] The invention also relates to a method for large-scale cultivation of microalgae by using the above-mentioned device. Background technique [0003] Microalgae can effectively use light energy, CO 2 and inorganic salts to synthesize proteins, fats, carbohydrates and a variety of high value-added bioactive substances, which can be used to produce health food, food additives, feed and other chemicals through microalgae cultivation. Especially in recent years with the development of bioenergy technology, some oily microalgae can be used in saline-alkali land, desert, Marine aquaculture has the great advantage of not competing for land and people for food, and can use carbon dioxide in factory exhaust gas as a carbon source. Countries all over the world have taken it as a very important way to solve the shortage of bi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00C12M1/42C12R1/89
CPCC12M21/02C12M23/04C12M23/08C12M29/02C12M29/20
Inventor 刘天中张维陈昱彭小伟陈晓琳陈林
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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