Trace substance detector with two fluorescence emission surfaces, and detection method

A technology of emission surface and double fluorescence, which is applied in the direction of fluorescence/phosphorescence, instruments, measuring devices, etc., can solve the problems of weakening the fluorescence intensity of the incident photodiode, difficulty in meeting the detection requirements of trace substances, loss of fluorescence intensity, etc., and achieve low detection The effect of extreme, high sensitivity, and less loss of fluorescence

Pending Publication Date: 2017-07-21
苏州尚稷电子科技有限公司
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  • Abstract
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  • Application Information

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Problems solved by technology

Because the dichromatic mirror set in the device loses part of the fluorescence intensity, and the optical path is longer, which weakens the fluo

Method used

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  • Trace substance detector with two fluorescence emission surfaces, and detection method
  • Trace substance detector with two fluorescence emission surfaces, and detection method
  • Trace substance detector with two fluorescence emission surfaces, and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Fluorescence intensity of a dual-band detector with single fluorescence emission surface and dual single fluorescence emission surface

[0028] The experimental conditions of the dual-band detector with single fluorescence emission surface: copper ion fluorescence probe (10 µM, compound 1 in Chinese invention patent ZL200410082745.3, maximum excitation wavelength 450 nm, probe emission 525 nm wavelength fluorescence, probe and Copper ions are combined to emit 475 nm wavelength fluorescence, LED excitation light source (wavelength 450 nm, rated power 40 mW), excitation power supply 40 mW, 50 mM HEPES buffered water-ethanol solution (pH 7.4, 30 o C), copper ion concentration (10 µM), square standard cuvette, various fluorescent condenser lenses, dichroic mirror (500 nm), A-channel fluorescence filter (475 nm, narrow band filter), B-channel Fluorescence filter (525 nm, band pass filter), condenser lens (plano-convex lens), magnification 4 M.

[0029] The experimental...

Embodiment 2

[0033] Example 2: Detection limit of copper ions detected by dual-band detector with single or dual fluorescence emission surfaces

[0034] The experimental conditions were the same as in Example 1.

[0035] Experimental results:

[0036]

[0037] The above results show that when the copper fluorescent probe is detected by an optical system with a single fluorescent emission surface, when the copper content drops from 0.03 mg / kg to 0.02 mg / kg, the fluorescence intensity of the A and B channels only changes by 1 mV, which is less than the photoelectric The sensitivity error range of the diode, therefore, the detection limit of this optical system is 0.03 mg / kg; when the copper fluorescent probe is detected by the optical system of the patented dual fluorescence emission surface, the copper content is increased from 0.015 mg / kg When the value reaches 0.020 mg / kg, the fluorescence intensity of the A and B channels changes by 7-9 mV, which is still within the sensitive range of the phot...

Embodiment 3

[0039] Such as figure 1 The working process of the detector shown is: the light emitted by the excitation light source 1 is irradiated to the cuvette 4 through the condenser lens 2 and the filter 3, and the detection fluorescent probe and the reference internal standard fluorescent dye in the cuvette are simultaneously Excited to emit fluorescence of different wavelengths; the fluorescence emitted by the detection fluorescent probe is converted into an electrical signal by the first photodiode 5a through the first fluorescence condenser lens 2a and the first fluorescence filter 3a in the first detection unit; The fluorescence emitted by the reference internal standard fluorescent dye is converted into an electrical signal by the second fluorescent photodiode 5b through the second fluorescent condenser lens 2b and the second fluorescent filter 3b in the second detection unit; in the two detections A and B The electrical signal obtained by the unit is amplified by the data acquis...

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Abstract

The invention discloses a trace substance detector with two fluorescence emission surfaces, and a detection method, and relates to an instrument used for detecting toxic and hazardous trace substances contained in environments, food, health care products, traditional Chinese medicines, cosmetics and the like in the technical field of spectrographic detection. The detector comprises an excitation unit, a first detection unit and a second detection unit, wherein the first detection unit is parallel to the second detection unit; the excitation unit is perpendicular to the first detection unit. Compared with a dual-waveband fluorescence intensity ratio detection system with a single fluorescence emission surface, the detector is not provided with a dichroic mirror or a fluorescence condensing lens on the basis of keeping the detection stability and an accuracy rate, so that a fluorescence loss is low; an optical path is short; the sensitivity is higher; a detection limit is lower. Under the equivalent condition of copper ion detection, by comparison of an optical system with two fluorescence emission surfaces and an optical system with a single fluorescence emission surface, the fluorescence detection strength is improved by 1.2 times and the detection limit is reduced from 0.03 mg/kg to 0.015 mg/kg. The detector is smaller in size and simple to operate, and can be used for on-site real-time quantitative rapid analysis and detection.

Description

Technical field [0001] The invention relates to a trace substance detector with dual fluorescence emission surfaces and a detection method, which belongs to the technical field of trace detection. Background technique [0002] The internal standard method commonly used in analytical chemistry is an indirect or relative calibration method. When analyzing the content of a certain component in the sample, an internal standard substance is added to calibrate and eliminate the influence of the fluctuation of the detection condition on the analysis result, so as to improve the accuracy of the analysis result and realize quantitative detection. The dual-path fluorescence detection system can simultaneously detect the fluorescence intensity emitted by the fluorescent dye as the reference internal standard and the fluorescence intensity emitted by the fluorescent probe associated with the detected object, to achieve ratiometric detection and eliminate the luminous intensity of the excitat...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428
Inventor 崔京南王铮崔文光
Owner 苏州尚稷电子科技有限公司
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