Method of preparing feather protein powder from keratinase

Abstract

The invention relates to the technical field of animal fodder processing, and especially relates to a method of preparing feather protein powder from keratinase. The method takes poultry feathers as the raw material and is characterized by comprising the following steps: (1) preparing feather powder; (2) performing enzymatic hydrolysis; (3) filtering and drying. The technology is simple, the suitable field of the technology is enlarged, the temperature of enzymatic hydrolysis is low, the pH range is wide, the cost of enzymatic hydrolysis is low, and the efficiency of enzymatic hydrolysis is high. The content of soluble protein in the supernate of enzymatic hydrolysis is high, the supernate contains 8 amino acids with a high content, wherein phenylalanine, isoleucine, leucine and valine are essential amino acids; the protein powder can be used to produce amino acid products and added into the feed, thus the production cost is reduced, and the comprehensive economic benefit is increased.

Description

technical field [0001] The invention relates to the technical field of animal feed processing, in particular to a method for preparing feather protein powder by using keratinase. Background technique [0002] Feather is one of the important resources in our country. The output is very rich, with an annual output of more than one million tons. It is composed of keratin with a very strong double bond structure. Its chemical composition is the same as that of ordinary proteins, but its molecular shape is similar to that of fibers and its structure is tight It is solid and dense, and it is difficult to be digested and absorbed by animals. It needs to be processed by physical or chemical methods to become a protein that can be digested and absorbed by animals. [0003] The air explosion enzymatic hydrolysis method is used to prepare feather protein powder. This method has complicated procedures and potential safety hazards. On the other hand, the amino acid in the feather powder ...

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Problems solved by technology

[0003] The air explosion enzymatic hydrolysis method is used to prepare feather protein powder. This method has complicated procedures and potential safety hazards. On the other hand, the amino acid in the feather powder is greatly damaged. can be used as feed protein

The applicant of this patent discloses a preparation method of feather protein powder in the patent application number 2014104846137, which uses keratinase / neutral protease to carry out compound enzymolysis on feathers. The most practical temperature is 40 degrees, which is not suitable for actual production. It is said that the energy consumption is high; since the pH range of keratinase is 7-10, and the pH range of neutral protease is 5.5-8.5, after the combination of the two enzymes, it is found that the optimum pH is 8, and the overlapping area of ​​action of the two enzymes Smaller, too much expense will be invested in the production for adjusting pH, and the addition amount of the most suitable enzyme is keratinase 0.0375 / neutral protease 0.125 g, consumption is higher, and these all can increase producer's production cost, economic benefit

In addition, due to the differences in the enzymatic hydrolysis sites of different proteases, the composition of amino acids in the enzymatic hydrolysis supernatant is also different. The composite enzymolysis of feathers with keratinase / neutral protease does not explain the difference in the enzymatic hydrolysis solution. Which amino acids are detected in the

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