Bio-organic fertilizer prepared from distillers' grains as well as preparation method and application of bio-organic fertilizer

A technology of bio-organic fertilizer and distiller's grains, applied in biochemical equipment and methods, microorganism-based methods, organic fertilizers, etc., can solve the problem of α-amylase activity and protease activity. Expose the application of distiller's grains in the field of decomposing, the variety of enzymes produced and the high enzyme activity, to achieve the effect of improving the quality of fruits and vegetables, high enzyme activity, and strong growth.

Active Publication Date: 2018-08-10
陕西枫丹百丽生物科技有限公司
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Problems solved by technology

[0013] Chinese patent CN200710064203 discloses a fertilizer starter and its preparation method and application. The raw material formula of the fertilizer starter includes: 10-80 parts of fermentation industrial waste residue, 5-30 parts of straw, 5-30 parts of cake, bran and 10-30 parts of rice bran, 1.5-2 parts of urea, 0.5-1 part of NaCl and / or glucose, and Azotobacter chroococcum, Bacillus megaterium, Bacillus licheniformis, Lactobacillus plantarum ( Lactobacillus plantarum) and 1-5 strains in Saccharomycopsis fibuligera) and Saccharomycopsis fibuligera. The function of Saccharomycopsis fibuligera disclosed in this patent is to quickly remove soil compaction, and Utilize the characteristics of wide range of substances, fast growth and easy decomposing, but the patent does not disclose the characteristics of acid resistance, rich enzyme production and high enzyme activity of the bacteria, and the characteristics of rich enzyme production and high activity are important indicators to ensure decomposing
[0022] The S. forticulata yeast described in the above patents is used in the brewing or feed fermentation industry, but its application in the field of distiller's grains is not disclosed; and the yeast S. forticulata used in the above patents has no activity except for α-amylase activity and protease activity. Whether it produces other enzymes and the activity of the enzyme is disclosed, and the application of the strain in fertilizer is not disclosed

Method used

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  • Bio-organic fertilizer prepared from distillers' grains as well as preparation method and application of bio-organic fertilizer
  • Bio-organic fertilizer prepared from distillers' grains as well as preparation method and application of bio-organic fertilizer
  • Bio-organic fertilizer prepared from distillers' grains as well as preparation method and application of bio-organic fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] The separation and purification, screening and identification of embodiment 1 yeast Y-1

[0059] 1) Separation and purification: Accurately weigh 10g of distiller's grains sample from the natural accumulation and fermentation of Wuliangye Group in Yibin City, Sichuan Province, add it to a sterilized triangular flask with glass beads containing 100mL of cooled sterile water, and shake it on a shaker at 200r / min Mix for 30 minutes, let it stand for 5 minutes, absorb 1 mL of the supernatant, and serially dilute to obtain 10 -1 -10 -5 Concentration, select 10 -1 、10 -2 、10 -3 、10 -4 、10 -5Use a pipette to absorb 100 μL of each gradient suspension, and spread it evenly on the YPD plate with streptomycin sulfate with a spreader. The final concentration of Streptomyces sulfate in the medium is 30 μg / mL. For bacterial water control, place the plate upside down in the incubator and incubate at 30°C for 7 days. Each treatment is set up to repeat 3 groups horizontally. Accor...

Embodiment 2

[0099] Example 2 Low pH Tolerance Test of Saccharomyces fornicata Y-1

[0100] (1) Method: Pick an appropriate amount of yeast Y-1 and insert it into the YPD liquid medium, and culture it with shaking at 180 rpm at 30°C for 48 hours to obtain the seed liquid. pH value (1.5, 2.0, 2.5, 3.0, 3.5, 4.0, 4.5, 5.0, 5.5, 6.0, 6.5) in the Erlenmeyer flask of sterilized YPD liquid medium, each pH value was repeated three times, and those without inoculation were Blank control, 30°C, 180r / min shaking culture for 48h, observe whether the bacterial liquid in the Erlenmeyer flask is turbid and turbid at each pH, ​​and measure the bacterial activity of the turbid bacterial liquid at the same time;

[0101] (2) Test results: Except for the pH values ​​of 1.0, 1.5, 2.0 and the CK group bacteria liquids were still clear, the YPD liquid culture media with different pH (2.5-6.5) were all turbid, indicating that S. It can grow within the range of pH 2.5-6.5, and the bacteria suspension with diffe...

Embodiment 3

[0102] Embodiment 3 solid-state fermentation of Saccharomyces fornicata Y-1

[0103] (1) Strain activation: use an inoculation loop to pick an appropriate amount of Saccharomyces fumigatus Y-1, streak it onto the PDA plate medium, and culture it at 30°C for 48 hours;

[0104] (2) Preparation of the primary seed liquid: pick an appropriate amount of activated Saccharomyces fornicosa Y-1 with an inoculation loop and inoculate it in the YPD liquid medium, and culture it with shaking at 200 rpm at 30°C for 24 hours to obtain the primary seed liquid;

[0105] (3) Preparation of the secondary seed liquid: inoculate the primary seed liquid into the YPD liquid medium again according to the inoculum amount of 1% (v / v), culture at 30° C. with shaking at 180 rpm for 36 hours, and obtain the secondary seed liquid;

[0106] (4) Solid-state fermentation research:

[0107] ① Mixing material: according to the formula of solid fermentation medium A, first use 500mL tap water to mix glucose, p...

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Abstract

The invention relates to bio-organic fertilizer prepared from distillers' grains as well as a preparation method and application of the bio-organic fertilizer, belonging to the technical field of bio-organic fertilizer. The preparation method of the bio-organic fertilizer prepared from the distillers' grains comprises the steps of adding one-thousandth biochemical potassium fulvate into decomposeddistillers' grains, evenly mixing, crushing the mixing by using a semi-wet material crusher, sieving with a 3-4mm rotary screen, then adding 5% jelly-like bacillus subtilis powder (living bacterium quantity: 10<9>cfu / g), 5% bacillus megaterium powder (living bacterium quantity: 10<10>cfu / g) and five-thousandth bacillus amyloliquefaciens powder (living bacterium quantity: 10<11>cfu / g). The decomposed distillers' grains microbial agent contains a preserved strain, i.e., saccharomycopsis fibuligera Y-1, and the preservation number of the preserved strain is China General Microbiological CultureCollection Center (CGMCC) No. 15255. The bio-organic fertilizer prepared from the distillers' grains can be used for various crops, is especially applied to winter jujubes, and can significantly improve the sizes, yield and quality of the fruits of the winter jujubes.

Description

technical field [0001] The invention relates to a distiller's grain bio-organic fertilizer, a preparation method and an application of the organic fertilizer, and belongs to the technical field of bio-organic fertilizers. Background technique [0002] Distiller's grains are the residual solids after cooking, saccharification and fermentation, distillation and other processes with sorghum, wheat, corn, rice, rye, etc. High acidity, high acidity, if it is allowed to discharge and is not treated in time, it will deteriorate and seriously pollute the environment. [0003] At present, many large-scale liquor companies choose to use distiller's grains for the second time, that is, to build steam workshops, and use the heat energy generated by burning distiller's grains as the source of heat consumed in liquor production. This is also a better technical choice for large-scale distiller's grains treatment. Energy use. However, it is necessary to solve the problem of secondary poll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00C12N1/16C12N1/14C12R1/645C12R1/685C12R1/69
CPCC05C9/00C05F9/04C12N1/14C12N1/16C05F11/02C05F11/08C05D9/00C05F5/008C05F17/20
Inventor 王灵敏李玲娣赵敬敬李爽王江
Owner 陕西枫丹百丽生物科技有限公司
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