Photo-thermal sensitive carboxymethyl chitosan nano drug-loaded microsphere and preparation method thereof

A technology of carboxymethyl chitosan and nano-drug loading, which is applied in the field of biomedical materials, can solve the problems of complexity, body stimulation, toxic and side effects, etc., and achieve the effect of increasing the loading rate, increasing the loading rate, and increasing the specific surface area

Inactive Publication Date: 2020-03-06
AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Another object of the present invention is to provide the preparation method of the above-mentioned photothermally sensitive carboxymethyl chitosan nano drug-loaded microspheres, by homogeneous emulsifying machine, simplifying the preparation method and combining nano gold with carboxymethyl chitosan with good biocompatibility The combination of base chitosan reduces the toxic and side effects, and further simplifies the loading technology to solve the problem of irritation and toxic side effects on the body due to the need for unmodified chitosan to be dissolved in an acid solution in some technologies, and to overcome the problem of loading nano The complication of gold-doxorubicin technical steps

Method used

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  • Photo-thermal sensitive carboxymethyl chitosan nano drug-loaded microsphere and preparation method thereof
  • Photo-thermal sensitive carboxymethyl chitosan nano drug-loaded microsphere and preparation method thereof
  • Photo-thermal sensitive carboxymethyl chitosan nano drug-loaded microsphere and preparation method thereof

Examples

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Embodiment 1

[0040] Embodiment 1 The oil-water ratio is the preparation of photothermally sensitive carboxymethyl chitosan nano drug-loaded microspheres of 10:1 1, the preparation of CMCS-NIPAm copolymer

[0041] Dissolve 0.24g carboxymethyl chitosan and 0.24g N-isopropylacrylamide with a mass ratio of 1:1 in 20mL deionized water, accelerate the dissolution with magnetic stirring, heat to 70°C under nitrogen protection, and slowly add 5mg 2mL of potassium persulfate / mL initiates the polymerization reaction, keeps at 70°C for 3h under nitrogen atmosphere, precipitates with acetone, centrifuges at 10000r / min for 10min, and vacuum-dries at 40°C to obtain carboxymethyl chitosan grafted N-iso Propylacrylamide polymer (CMCS-NIPAm copolymer).

[0042] Carry out Fourier transform infrared characterization to the CMCS-NIPAm copolymer prepared in embodiment 1, scanning result is as follows Figure 4 As shown, the spectral characteristics of CMCS are as follows: 3427cm -1 Telescoping for O-H and N-...

Embodiment 2

[0051] Example 2 The preparation of photothermal-sensitive carboxymethyl chitosan nano drug-loaded microspheres with an oil-water ratio of 20:1 1. Preparation of CMCS-NIPAm copolymer

[0052] Dissolve 0.24g carboxymethyl chitosan and 0.24g N-isopropylacrylamide with a mass ratio of 1:1 in 20mL deionized water, accelerate the dissolution with magnetic stirring, heat to 70°C under nitrogen protection, and slowly add 5mg 2mL of potassium persulfate / mL initiates the polymerization reaction, keeps at 70°C for 3h under nitrogen atmosphere, precipitates with acetone, centrifuges at 10000r / min for 10min, and vacuum-dries at 40°C to obtain carboxymethyl chitosan grafted N-iso Propylacrylamide polymer (CMCS-NIPAm copolymer).

[0053] Similarly, Fourier infrared characterization was performed on the CMCS-NIPAm copolymer prepared in Example 2 to ensure that N-isopropylacrylamide was successfully grafted onto carboxymethyl chitosan.

[0054] 2. Preparation of photothermally sensitive carb...

Embodiment 3

[0060] Example 3 Preparation of photothermally sensitive carboxymethyl chitosan nano drug-loaded microspheres with an oil-water ratio of 30:1

[0061] 1. Preparation of CMCS-NIPAm copolymer

[0062] Dissolve 0.24g carboxymethyl chitosan and 0.24g N-isopropylacrylamide with a mass ratio of 1:1 in 20mL deionized water, accelerate the dissolution with magnetic stirring, heat to 70°C under nitrogen protection, slowly drop 5mg / 2 mL of potassium persulfate was used to initiate the polymerization reaction, kept at 70°C for 3 h under nitrogen atmosphere, precipitated with acetone, centrifuged at 10,000 r / min for 10 min, and dried under vacuum at 40°C to obtain carboxymethyl chitosan grafted with N-isopropyl Based acrylamide polymer (CMCS-NIPAm copolymer).

[0063] Similarly, Fourier transform infrared characterization was performed on the CMCS-NIPAm copolymer prepared in Example 3 to ensure that N-isopropylacrylamide was successfully grafted onto carboxymethyl chitosan.

[0064] 2....

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Abstract

The invention belongs to the technical field of biomedical materials, and particularly relates to a photo-thermal sensitive carboxymethyl chitosan nano drug-loaded microsphere, a nano microsphere is formed by taking a polymer carboxymethyl chitosan/N-isopropylacrylamide as a skeleton and loading hydrazone bond bonded nanogold-doxorubicin hydrochloride on the surface of the skeleton. The inventionalso provides a preparation method of the nano drug-loaded microsphere. The invention provides the photo-thermal sensitive carboxymethyl chitosan nano drug-loaded microsphere, the problem that chitosan is difficult to dissolve in water is solved, meanwhile, by changing the conditions of near-infrared illumination and temperature, the nano microsphere material is subjected to phase change, hydrophilic chain collapse and solvation layer damage, a medicine is slowly released, the medicine carrying rate of the medicine is increased, and the medicine is controlled to be accurately released.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a photothermal-sensitive carboxymethyl chitosan nano drug-loaded microsphere and a preparation method thereof. Background technique [0002] Sensitive nanocarriers are a class of nanomaterials that can respond to external physical and chemical stimuli (such as light, ultrasound, magnetic field, pH, etc.), and change their structure and performance to promote or inhibit drug release. It has become a new way of tumor-targeted drug delivery. The use of sensitive nanocarriers as controlled-release drug carriers has become an upsurge, and most of the controlled-release drug carriers are unsatisfactory in slow-release control and drug loading capacity due to improper selection of materials, complex preparation methods, and improper operations during the preparation process. [0003] In terms of material selection, carboxymethyl chitosan is widely cited as a dr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/36A61K31/704A61K41/00A61P35/00C08F251/00C08F220/54C08L51/02C08J3/24
CPCA61K9/5161A61K9/5192A61K31/704A61K41/0052A61P35/00C08F251/00C08J3/24C08J2351/02A61K2300/00C08F220/54
Inventor 杨子明李普旺陈煜冯长根周闯何祖宇王超刘显武吕明哲刘运浩
Owner AGRI PRODS PROCESSING RES INST CHINESE ACAD OF TROPICAL AGRI SCI
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